Comparative Mitogenomic Analysis of Two Longhorn Beetles (Coleoptera: Cerambycidae: Lamiinae) with Preliminary Investigation into Phylogenetic Relationships of Tribes of Lamiinae

Abstract

The subfamily Lamiinae is the most taxonomically diverse subfamily of Cerambycidae, but relationships between tribes of Lamiinae are still unresolved. In order to study the characteristics of the mitogenomes of Lamiinae and the tribal-level phylogenetic relationships, we sequenced the mitogenomes of two species representing two tribes, Agapanthia amurensis (Agapanthiini) and Moechotypa diphysis (Ceroplesini), with a total length of 15,512 bp and 15,493 bp, respectively. The gene arrangements of these two new mitogenomes were consistent with the inferred ancestral insect mitogenomes. Each species contained 37 typical mitochondrial genes and a control region (A + T-rich region), including 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), and two ribosomal RNA genes (rRNAs). All PCGs initiated with the standard start codon ATN, and terminated with the complete stop codons of TAA and TAG, or incomplete stop codon T. All tRNAs could be folded into a clover-leaf secondary structure except for trnS1, in which the dihydrouridine (DHU) arm was reduced. Moreover, we studied the phylogenetic relationships between some tribes of Lamiinae based in mitochondrial PCGs in nucleotides; our results show that the relationships were as follows: (Onciderini + ((Apomecynini + Acanthocinini) + ((Ceroplesini + Agapanthiini) + ((Mesosini + Pteropliini) + ((Dorcaschematini + (Saperdini 1 + (Phytoeciini + Saperdini 2))) + (Batocerini + Lamiini)))))).

Time-scaled phylogenetic analysis of extant Lamiinae (Coleoptera, Cerambycidae) species of East of Marmara Basin, Türkiye, and their evolutionary similarity with the Eurasian congeners

“…According to our chronogram, the MRCA of Saperdini and Phytoeciini was 53.21 (95% HPD: 80-34) million years old. Same as Ren et al (2021), our findings support the suggestion of de Santana Souza et al (2020) that the tribe Phytoeciini should also be a synonym of Saperdini or all members should be evaluated separately.…”

“…2). Ren et al (2021) is another recent study, supported the sisterships of Batocerini and Lamiini. The MRCA of the group, which included Monochamus and Batocera , appeared around 63 mya at the chronogram of Ashman et al (2022), 54.9 mya of Nie et al (2021), and 47.47 (95% HPD: 63-36) mya at our chronogram, Eocene epoch of Paleogene.…”

“…de Santana Souza et al (2020) studied 46 tribes by analysing the fragments of two mitochondrial and three nuclear markers and inferred that some tribes such as Astathini, Batocerini, Ceroplesini, Colobotheini, Compsosomatini, Dorcadionini, Lamiini, Mesosini, Obereini and Polyrhaphidini are monophyletic; but, Acanthocinini, Acanthoderini, Agapanthiini, Apomecynini, Desmiphorini, Dorcaschematini, Enicodini, Hemilophini, Monochamini, Onciderini, Parmenini, Phytoeciini, Pogonocherini, Pteropliini and Saperdini are not. Ren et al (2021) analysed 13 protein-coding genes of mitogenomes of representatives of 12 tribes. They suggested the sisterships of Ceroplesini and Agapanthiini, Mesosini and Pteropliini, Saperdini and Phytoeciini, Batocerini and Lamiini, and Apomecynini and Acanthocinini.…”

Comparative mitogenomic analysis provides evolutionary insights into Formica (Hymenoptera: Formicidae)

“…The use of mitogenomic data is a common approach to exploring phylogenetic relationships among different insect groups [ 68 , 69 ]. The sister relationship between Formica and Polyergus ants confirmed in the present work was largely congruent with the results of previous studies [ 6 ].…”

The Phylogenetic Relationship of Lamiinae (Coleoptera: Cerambycidae) Using Mitochondrial Genomes

“…To date, eighty complete or partial mitochondrial genomes of Lamiinae (≥13,854 bp) have already been published in the NCBI [10,11,[35][36][37][38][39][40][41][42][43][44][45][46][47][48][49][50]. The position of the Lamiinae subfamily has been clearly delineated, but the relationships of Lamiinae tribes remain vague [17,51]. For our present study, we acquired mitochondrial genome sequences for 147 Coleopteran species from the NCBI and combined these with the genome data for the 11 Lamiinae species provided in the current study.…”