Comparative morphological and growth kinetics studies of human hair bulb papilla cells and root sheath fibroblasts in vitro

Katsuoka, Kensei; Schell, H.; Hornstein, O. P.; Deinlein, E; Wessel, B.

doi:10.1007/bf00404353

Cited by 50 publications

(14 citation statements)

References 13 publications

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“…Follicular papilla cells were isolated and cultured according to the established method (Jahoda and Oliver, 1981;Messenger, 1984;Williams et al, 1994). The cells in serial sub-cultures have a polygonal cellular morphology and a tendency to form multi-layered aggregates distinct from dermal fibroblasts (Messenger et al, 1986;Katsuoka et al, 1986;Tobin et al, 1991), and have significantly higher a-actin expression than dermal fibroblasts Reynolds et al, 1993a). The isolation and subculture of hair bulb-derived keratinocytes was performed by following the recent methods for isolation of hair matrix cells (Kurata et al, 1991;Reynolds et al, 1993b;Limat et al, 1993b;Detmar et al, 1993).…”

Section: Discussionmentioning

confidence: 99%

Hepatocyte growth factor/scatter factor expressed in follicular papilla cells stimulates human hair growth in vitro

Shimaoka

Tsuboi

Jindo

et al. 1995

Journal Cellular Physiology

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Hepatocyte growth factor/scatter factor (HGF/SF) is a multifunctional polypeptide which acts as mitogen, motogen, or morphogen. In this study, we examined the effect of HGF/SF on human hair growth using organ and cell culture systems. HGF/SF was found to stimulate hair length and DNA synthesis in hair follicles at increasing concentrations up to 10 ng/ml (P < 0.05 and P < 0.01, respectively). HGF/SF stimulated [3H]thymidine incorporation by hair bulb-derived keratinocytes with the strongest response at 30 ng/ml of HGF/SF (P < 0.05). Cultured follicular papilla cells secreted HGF/SF, measured by an enzyme-linked immunoassay, in response to interleukin 1-alpha (IL1-alpha, 10 ng/ml), tumor necrosis factor-alpha (TNF-alpha, 10 ng/ml), or tetradecanoylphorbolacetate (100 nM) at levels ranging from 0.2 to 0.3 ng/mg protein/48 h. HGF/SF mRNA expressions, measured by the reverse transcription-polymerase chain reaction, were detected in follicular papilla cells, and were also stimulated by the three reagents. Transforming growth factor-beta (10 ng/ml) suppressed both protein and mRNA levels. These results suggest that hair follicle elongation induced by HGF/SF in organ culture occurs partly due to the mitogenic activity of HGF/SF expressed in follicular papilla cells on hair bulb-derived keratinocytes.

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Section: Discussionmentioning

confidence: 99%

Hepatocyte growth factor/scatter factor expressed in follicular papilla cells stimulates human hair growth in vitro

Shimaoka

Tsuboi

Jindo

et al. 1995

Journal Cellular Physiology

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“…Scalp punch biopsies were taken from frontal and oc cipital regions of stump-tailed macaques (5 males and 5 females). The methods for isolation and culture of the follicular cells, dermal papilla and outer root sheath cells were described elsewhere [9][10][11][12]. For prep aration of the perifollicular fibrocytes, using a manual ly dissected single anagen follicle, the bulb portion of the follicle was cut off, then the follicular connective tissue sheath was separated.…”

Section: Methodsmentioning

confidence: 99%

Effects of Hypertrichotic Agents on Follicular and Nonfollicular Cells in vitro

Kurata¹,

Uno

Allen-Hoffmann

1996

Skin Pharmacol Physiol

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Our previous studies revealed that topical minoxidil induced an increased rate of DNA synthesis in both dermal papilla and follicular germinal cells in early anagen and bulbar matrix as well as outer root sheath and perifollicular fibrocytic cells in mid and late anagen follicles in the bald scalp of the stump-tailed macaque. However, the epidermis and dermal fibrocytes showed no response. To determine the specific action of hypertrichotic agents on follicular cells, we examined the effects of two potent hypertrichotic agents, minoxidil and cyclosporin, on the DNA synthesis of cultured cells derived from either follicular cells (dermal papular, perifollicular fibrocytic and outer root sheath cells) obtained from human and macaque scalps or nonfollicular cells (fibrocytes and epidermal keratinocytes) from human and macaque foreskin, palm and sole regions and the 3T3 cell line. Cultured subconfluent cells from the above follicular and nonfollicular specimens were incubated with either minoxidil (0.01-2 mM) or cyclosporin (0.01-100 mM) in medium (serum-free DMEM) for 48 h, then ³H-fhymidine was added for the final 6 h. Minoxidil induced a significant increase in DNA synthesis in all follicular cells in a dose-specific manner (maximum rate at 0.5 mM for dermal papilla and perifollicular fibrocytic cells and 0.1 mM for outer root sheath cells). The perifollicular fibrocytic cells appeared to have a potentiality similar to that of the dermal papilla cells. Nonfollicular cells showed no response to minoxidil; 3T3 cells were rather suppressed. Cyclosporin appeared to have rather suppressive effects on both follicular and nonfollicular cells. These results suggest that minoxidil has a specific affinity to hair follicular cells and induced their cell proliferation. Although cyclosporin is known as a potent hypertrichotic agent, our studies on cultured follicular cells showed no direct proliferative effect. The hypertrichotic mechanism of cyclosporin appeared to be different from that of minoxidil.

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“…Up to this point, there have been some research reports in which the numbers of outer root sheath cells or dermal papilla cells were increased by culture (4,8). Hair growth was induced by the implantation of cultured dermal papilla cells (3).…”

Section: Discussionmentioning

confidence: 96%

“…The main methods could be classified as either culturing of individual follicular cells (4,8) or maintaining of intact follicles (7). A third method has also been described (5,6), in which dissociated cells derived from some tissues and organs could reform the original tissues or organs by rotation culture under proper conditions.…”

mentioning

confidence: 99%

Histodifferentiation of Hair Follicles in Grafting of Cell Aggregates Obtained by Rotation Culture of Embryonic Rat Skin

Matsuhashi

Shioya²,

Ihara³

1998

Scandinavian Journal of Plastic and Reconstructive Surgery and

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We have previously reported reconstruction of hair follicles from a single cell suspension of rat fetal upper lip by a two-step culture method consisting of rotation and flotation cultures. Rotation sorted out the cells and flotation facilitated histodifferentiation. In the present study, we added grafting procedures to the previous method to see whether cell aggregates obtained this way were graftable, and whether the grafting promoted histodifferentiation. The aggregates before and after flotation were grafted, and differentiation of hair follicles comparable to those in vivo was confirmed 10 days after grafting. There was no difference in the degree of differentiation between the two kinds of grafts. The grafting procedure therefore resulted in an appreciable increase in histodifferentiation even when aggregates obtained after flotation were grafted.

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Comparative morphological and growth kinetics studies of human hair bulb papilla cells and root sheath fibroblasts in vitro

Cited by 50 publications

References 13 publications

Hepatocyte growth factor/scatter factor expressed in follicular papilla cells stimulates human hair growth in vitro

Hepatocyte growth factor/scatter factor expressed in follicular papilla cells stimulates human hair growth in vitro

Effects of Hypertrichotic Agents on Follicular and Nonfollicular Cells in vitro

Histodifferentiation of Hair Follicles in Grafting of Cell Aggregates Obtained by Rotation Culture of Embryonic Rat Skin

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