Comparative morphology of myrmecophilous immature stages of European Microdon species (Diptera: Syrphidae): updated identification key and new diagnostic characters

Abstract

Hoverflies (Diptera: Syrphidae) of the genus Microdon Meigen have larvae that live in ant nests where they are predatory on ant larvae. Reflecting the exceptional challenges of this very specialized lifestyle, Microdon eggs, larvae and puparia are highly distinctive in their morphology. Detailed descriptions of these immature stages is, however, lacking for all but a very few species, and much of this has been limited through the sole use of light microscopes. Here, using Scanning Electron Microscopy (SEM), we present detailed, comparative descriptions of the immature stages of three European Microdon species: M. analis, M. devius and M. myrmicae. Given that many adult Microdon species are very similar to each other in their outward appearance, we demonstrate that the morphology of their immature stages can improve our understanding of the phylogeny of the genus. We also discuss how particular adaptations of the immature morphology may allow their myrmecophilous life within ant nests. In this paper new diagnostic features are also presented to distinguish M. myrmicae from its sibling species M. mutabilis—the two are morphologically indistinguishable as adults.

Social parasite distancing: RADseq reveals high inbreeding in the social parasite Microdon myrmicae but low philopatry for host ant nest

“…fuscipennis (Macquart) placed in ant colonies face a mortality rate of 90% or even more. Other laboratory breeding ended with the same mortality rates (Witek et al, 2012;Scarparo et al, 2017Scarparo et al, , 2020. Furthermore, it has been demonstrated that high inbreeding levels drastically reduce larval survival and egg-hatching rates, contributing to the local extinction of small populations (Saccheri et al, 1998).…”

“…mutabilis , based on its affiliation with a different host genus ( Myrmica instead of Formica ), and on the length of the prothoracic horns (respiratory organs emerging anterodorsally during the pupal stage) (Schönrogge et al ., 2002). Further morphological diagnostic characters to distinguish the immature stages of these two species have been recently described (Scarparo et al ., 2020). Microdon myrmicae is mostly found in association with Myrmica scabrinodis , although many other Myrmica species are known to be suitable hosts ( e.g ., My.…”