Comparative nucleotide sequencing of the VP1 capsid gene of recent isolates of foot-and-mouth disease virus serotype O from Egypt

Abu-Elnaga, Hany; Rizk, Sonia A.; Daoud, Hind M.; Mohamed, Assem A; Mossad, W G; Gamil, Mohamed A; Soudy, Ahmed F.; El-Shehawy, L.

doi:10.1007/s00705-020-04708-1

Cited by 8 publications

(6 citation statements)

References 35 publications

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“…-3) topotype. As the result of these findings, the author's recommendations to incorporate EA-3 into vaccine strains these results in close agreement with the findings of AbuElnaga et al (34), who concluded that any vaccine containing the Pan-Asia-2 virus support full or partial protection against the Sharqia-72 virus or vice versa. At the same time, any vaccine containing the Pan-Asia-2 virus or a Sharqia-72 virus may support partial or no protection against EA-3 viruses.…”

Section: Discussionsupporting

confidence: 87%

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Genetic characterization and phylogenetic analysis of foot and mouth disease virus vaccine strains and recent field isolate

El-Bagoury¹,

El-Deen²,

Fakhry³

2023

IJVS

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Foot and Mouth Disease (FMD) control is a national and regional responsibility. FMD outbreaks were detected in three farms in Port-Said Governorate in 2020, although animals in these farms were vaccinated with the local polyvalent inactivated vaccine. Samples from tongue epithelium and vesicular fluid were collected from these farms, where FMDV isolation was performed on BHK-21 cell culture. Viral RNA was extracted from the virus isolates, and vaccine strains were then screened for FMDV using conventional Reverse transcriptase polymerase chain reaction. The substitution rates and phylogenetic relationship between the field isolate and the vaccine strains were determined using DNA sequence and bioinformatics analyses. The results illustrate that the sequence analysis for the 1D region of FMDV field isolate was serotype A-Africa topotype, Genotype IV, and closely related to isolated A-Africa topotype Genotype IV by Animal Health Research Institute, 2020 with nucleotide similarity ranging from 97.1% to 99.74%, and revealing genetic variation of 5.63-7.88% from previously Egyptian isolated A-Africa topotype, Genotype IV in 2016 and 2018. High genetic variations were determined from the locally used vaccine strain serotype A of the Asia topotype, lineage Iran-05, in the major antigenic sites of the VP1 region with a nucleotide difference of 26.34%. Depending on these findings, the Veterinary Serum and Vaccine Research Institute produced an emergency inactivated monovalent vaccine using the newly isolated strain, and we recommended adding it to the subsequent prepared vaccine batches.

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Section: Discussionsupporting

confidence: 87%

“…The relationship between the FMDV circulating strains in Egypt and vaccine strains (5,6,9,34,(38)(39)(40)(41). A-FMDV circulating strains within serotype A: (1-Topotype Africa G-VII and G-IV&2-Topotype Asia, lineage Iran-05).…”

Section: Discussionmentioning

confidence: 99%

Genetic characterization and phylogenetic analysis of foot and mouth disease virus vaccine strains and recent field isolate

El-Bagoury¹,

El-Deen²,

Fakhry³

2023

IJVS

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“…These were also very closely related to O/EGY/8/2006 (KR149727; 99.7% nucleotide identity in 1D/VP1, one silent mutation and one amino acid exchange). The clinical isolates O/EGY/H1-BTE/2005 (accession# MN296504) and O/EGY/H2-BFTE/2005 (accession# MN296504) were again identical and even more similar (one amino acid exchange in VP1) to the much older O 1 /Sharquia/EGY/72 isolate, as has been previously noted by Abu-Elnaga et al [46]. While this may be a result of widespread laboratory contamination and misidentification of isolates, it appears unlikely considering the diversity of researchers and institutions that have contributed to the database.…”

Section: Commentedsupporting

confidence: 76%

Sequence Analysis of Egyptian Foot-and-Mouth Disease Virus Field and Vaccine Strains: Intertypic Recombination and Evidence for Accidental Release of Virulent Virus

Rahman

Hoffmann

Karam

et al. 2020

Viruses

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In spite of annual mass vaccination programs with polyvalent inactivated vaccines, the incidence and economic impact of foot-and-mouth disease (FMD) in Egypt is high. Viruses of the A, O and SAT 2 serotypes are endemic and repeated incursions of new lineages from other countries lead to an unstable situation that makes the selection of appropriate vaccine antigens very difficult. In this study, whole genome sequencing of a 2016 serotype A isolate from Egypt revealed a recombination event with an African serotype O virus. Based on available vaccine matching data, none of the vaccines currently used in Egypt are expected to sufficiently protect against this virus or other viruses of this lineage (A/AFRICA/G-IV) circulating there since 2012. In addition to the risk of vaccine failure caused by strain mismatch, the production of inactivated FMD vaccines is dangerous if adequate biosafety cannot be maintained. Using a high-throughput sequencing protocol optimized for short nucleic acid fragments, the composition of a local inactivated vaccine was analyzed in depth. The serotype O strain identified in the vaccine was genetically identical to viruses found in recent FMD outbreaks in Egypt.

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“…These are also very closely related to O/EGY/8/2006 (KR149727) (99.7% nucleotide identity in 1D/VP1, one silent mutation and one amino acid exchange). The clinical isolates O/EGY/H1-BTE/2005 (accession# MN296504) and O/EGY/H2-BFTE/2005 (accession# MN296504) are again identical and even more similar (one amino acid exchange in VP1) to the much older O1/Sharquia/EGY/72 isolate, as has been previously noted by Abu-Elnaga et al [41]. While this may be a result of widespread laboratory contamination and misidentification of isolates, it appears unlikely considering the diversity of researchers and institutions that have contributed to the database.…”

Section: Composition Analysis Of 2019 Inactivated Fmdv Vaccinesupporting

confidence: 75%

Sequence Analysis of Egyptian Foot-And-Mouth Disease Virus Field and Vaccine Strains: Intertypic Recombination and Evidence for Accidental Release of Virulent Virus

Rahman¹,

Hoffmann²,

Karam³

et al. 2020

Preprint

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In addition to the risk of vaccine failure caused by strain mismatch, the production of inactivated FMD vaccines is dangerous if adequate biosafety cannot be maintained. Using a high-throughput sequencing protocol optimized for short nucleic acid fragments, the composition of a local inactivated vaccine was analyzed in depth. The serotype O strain identified in the vaccine was genetically identical to viruses found in recent FMD outbreaks in Egypt.

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Comparative nucleotide sequencing of the VP1 capsid gene of recent isolates of foot-and-mouth disease virus serotype O from Egypt

Cited by 8 publications

References 35 publications

Genetic characterization and phylogenetic analysis of foot and mouth disease virus vaccine strains and recent field isolate

Genetic characterization and phylogenetic analysis of foot and mouth disease virus vaccine strains and recent field isolate

Sequence Analysis of Egyptian Foot-and-Mouth Disease Virus Field and Vaccine Strains: Intertypic Recombination and Evidence for Accidental Release of Virulent Virus

Sequence Analysis of Egyptian Foot-And-Mouth Disease Virus Field and Vaccine Strains: Intertypic Recombination and Evidence for Accidental Release of Virulent Virus

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