Comparative performance of fetal goat tongue cell line ZZ-R 127 and fetal porcine kidney cell line LFBK-αvβ6 for Foot-and-mouth disease virus isolation

Fukai, Katsuhiko; Morioka, Kazuki; Yamada, Manabu; Nishi, Tatsuya; Yoshida, Kazuo; Kitano, Rie; Yamazoe, Reiko; Kanno, Toru

doi:10.1177/1040638715584156

Cited by 22 publications

(21 citation statements)

References 12 publications

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“…Monolayers of a fetal porcine kidney cell line constitutively expressing α v β 6 integrin (LFBKα v β 6 cells, [18][19] were inoculated with tissue suspensions and environmental samples, the plates incubated for 2–3 days at 37° C and checked for cytopathic effect (CPE). If CPE was observed, supernatants were collected and tested for SVA by RRT-PCR.…”

Section: Methodsmentioning

confidence: 99%

Genome wide analysis of the evolution of Senecavirus A from swine clinical material and assembly yard environmental samples

Hole

Goolia

et al. 2017

PLoS ONE

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Senecavirus A (SVA), previously known as Seneca Valley virus, was first isolated in the United States in 2002. SVA was associated with porcine idiopathic vesicular disease in Canada and the USA in 2007 and 2012, respectively. Recent increase in SVA outbreaks resulting in neonatal mortality of piglets and/or vesicular lesions in sows in Brazil, the USA and Canada point to the necessity to study the pathogenicity and molecular epidemiology of the virus. Here, we report the analysis of the complete coding sequences of SVA from 2 clinical cases and 9 assembly yard environmental samples collected in 2015 in Canada, along with 22 previously released complete genomes in the GenBank. With this combined data set, the evolution of the SVA over a 12-month period in 2015/2016 was evaluated. These SVA isolates were characterized by a rapid accumulation of genetic variations driven mainly by a high nucleotide substitution rate and purifying selection. The SVA sequences clustered in clearly defined geographical areas with reported cases of SVA infection. No transmission links were identified between assembly yards, suggesting that point source introductions may have occurred. In addition, 25 fixed non-synonymous mutations were identified across all analyzed strains when compared to the prototype SVA strain (SVV-001). This study highlights the importance of monitoring SVA mutations for their role in increased virulence and impact on SVA diagnostics.

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Section: Methodsmentioning

confidence: 99%

Genome wide analysis of the evolution of Senecavirus A from swine clinical material and assembly yard environmental samples

Hole

Goolia

et al. 2017

PLoS ONE

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“…The cell density was adjusted to form a 70% monolayer, and the test was conducted by using a two-fold dilution of sera. Following incubation of the test serum with FMDV (O/Andong/SKR/2010 [=O/SKR(KOR)/Nov/2010, AD] or O/Jinchon/SKR/2014 [=O/SKR(KOR)/Dec/2014, JC]) 100 tissue culture infectious dose (TCID) 50 /0.05 mL for 1 h [ 17 ], LF-BK (bovine kidney) cells were added to the plate and incubated for 2–3 days [ 6 ]. The CPE was checked to determine the titers, which were calculated as the log 10 of the reciprocal antibody dilution to neutralize 100 TCID 50 of virus [ 5 ].…”

Section: Methodsmentioning

confidence: 99%

Immune responses in pigs and cattle vaccinated with half-volume foot-and-mouth disease vaccine

et al. 2017

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With the current commercial foot-and-mouth disease vaccine, inoculating twice increases the formation of denatured meat due to granuloma or residual adjuvant at the injection site in pigs, resulting in economic loss. Therefore, we investigated protective antibody levels after reducing the amount of adjuvant in the vaccine. Field applicability of the experimental vaccine, made with a new adjuvant ISA 201, was tested by vaccinating farm animals with half-volume doses (1 mL/animal) of commercial vaccine and monitoring their immunogenicity. Among pigs, the group that received a half-volume dose showed similar or higher titers of structural protein antibody and neutralizing antibody than those receiving the standard dose (2 mL). In pigs, the durable effects of antibody titer of the reduced vaccine volume did not diminish up to the time of slaughter. Among cattle, boosting with a second 1 mL vaccine increased virus neutralizing antibody for the protective effects. The boosting effects were more marked in cattle than in pigs. The immune responses differed between species with the effect of the half-volume vaccination being lower in cattle than in pigs. In conclusion, the immune response to the half-volume vaccine was similar to that from the standard volume vaccine in pigs, but not in cattle.

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“…Virus isolation was performed as described previously (Fukai et al, ). Freshly prepared confluent monolayers of cells in 24‐well plates were washed once and incubated with 150μl of clinical samples for 1 hr at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Horizontal transmission of foot‐and‐mouth disease virus O/JPN/2010 among different animal species by direct contact

Fukai

Nishi

Morioka

et al. 2019

Transbound Emerg Dis

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Foot‐and‐mouth disease (FMD) is highly contagious and easily transmitted among species of cloven‐hoofed animals. To investigate the transmission of FMD virus (FMDV) among different animal species, experimental infections using the O/JPN/2010 strain were performed in cows, goats and pigs. One cow or two goats/pigs were housed with a different species of inoculated animals, and clinical observations, virus shedding and antibody responses were analysed daily. Whilst all cows and goats were infected horizontally by contact with inoculated pigs, transmission from cows to goats/pigs and from goats to cows/pigs was not observed in all in‐contact animals. In particular, no pigs were infected horizontally by contact with inoculated goats. Comparison with our previous study on experimental infections among animals of the same species indicates that horizontal transmission occurred more easily between animals of the same species than between those of the different species. These findings will be useful for establishing and performing species‐specific countermeasures in farms and regions where multiple species of animals coexist in potential future outbreaks.

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Comparative performance of fetal goat tongue cell line ZZ-R 127 and fetal porcine kidney cell line LFBK-α_vβ₆ for Foot-and-mouth disease virus isolation

Cited by 22 publications

References 12 publications

Genome wide analysis of the evolution of Senecavirus A from swine clinical material and assembly yard environmental samples

Genome wide analysis of the evolution of Senecavirus A from swine clinical material and assembly yard environmental samples

Immune responses in pigs and cattle vaccinated with half-volume foot-and-mouth disease vaccine

Horizontal transmission of foot‐and‐mouth disease virus O/JPN/2010 among different animal species by direct contact

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