Comparative performance study of polyethersulfone and polysulfone membranes for trypsin isolation from goat pancreas using affinity ultrafiltration

Mukherjee, Shravanti; Roy, Debashis; Bhattacharya, Pinaki

doi:10.1016/j.seppur.2007.12.013

Cited by 11 publications

(7 citation statements)

References 41 publications

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“…In order to use these enzymes it is necessary to isolate them and to preserve them properly to keep their functionality. There are a lot of methodologies to obtain these serine proteases in large quantities but most of them are expensive or generate byproducts that are not suitable to be discarded [6][7][8][9][10]. We have proposed different methods to isolate ChTRP [11][12][13][14], Tryp [15,16] and their zymogens [17,18].…”

Section: Introductionmentioning

confidence: 99%

“…Conventional multistep downstream processing of enzymes is often time consuming, labor intensive, requiring huge hold-up volumes to operate in batch or semibatch mode and usually accounts for up to 80% of the total process costs [6,[18][19][20]. Some of the traditional methods suitable to be applied in scaling up are ion exchange adsorption [21] or chromatography [22].…”

Section: Introductionmentioning

confidence: 99%

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Obtainment of a highly concentrated pancreatic serine proteases extract from bovine pancreas by precipitation with polyacrylate

Lombardi

Valetti

Picó

et al. 2013

Separation and Purification Technology

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Obtainment of a highly concentrated pancreatic serine proteases extract from bovine pancreas by precipitation with polyacrylate

Lombardi

Valetti

Picó

et al. 2013

Separation and Purification Technology

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“…A single flat sheet functionalized membrane is rarely fitted in the membrane module of MC systems [66,67]. A stack of functionalized membranes with a lower surface area (~7.5-85 cm 2 ) and bed volume (~240 µL−2.7 mL) as a stationary phase is used in spincolumn MC systems.…”

Section: Axial Flow Membrane Modulementioning

confidence: 99%

Membrane Chromatography and Fractionation of Proteins from Whey—A Review

et al. 2022

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Membrane chromatography (MC) is an emerging bioseparation technology combining the principles of membrane filtration and chromatography. In this process, one type of molecule is adsorbed in the stationary phase, whereas the other type of molecule is passed through the membrane pores without affecting the adsorbed molecule. In subsequent the step, the adsorbed molecule is recovered by an elution buffer with a unique ionic strength and pH. Functionalized microfiltration membranes are usually used in radial flow, axial flow, and lateral flow membrane modules in MC systems. In the MC process, the transport of a solute to a stationary phase is mainly achieved through convection and minimum pore diffusion. Therefore, mass transfer resistance and pressure drop become insignificant. Other characteristics of MC systems are a minimum clogging tendency in the stationary phase, the capability of operating with a high mobile phase flow rate, and the disposable (short term) application of stationary phase. The development and application of MC systems for the fractionation of individual proteins from whey for investigation and industrial-scale production are promising. A significant income from individual whey proteins together with the marketing of dairy foods may provide a new commercial outlook in dairy industry. In this review, information about the development of a MC system and its applications for the fractionation of individual protein from whey are presented in comprehensive manner.

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“…Polyethersulfone (PES) and polysulfone (PSF) are the most common membranes used in clinical applications and represent 93% of the market [ 4 , 5 , 6 ]. PES has a higher clearance efficiency compared to PSF [ 7 ]. Nevertheless, PES membranes still contain shortcomings, including limited hemocompatibility [ 1 , 8 , 9 ], which triggers blood activation cascades, as the rate of morbidity and mortality in ESRD patients is still unacceptably high [ 3 , 10 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Aminolysis-Based Zwitterionic Immobilization on Polyethersulfone Membranes for Enhanced Hemocompatibility: Experimental, Computational, and Ex Vivo Investigations

Mollahosseini,

Bahig,

Shoker

et al. 2024

Biomimetics

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Dialysis membranes are not hemocompatible with human blood, as the patients are suffering from the blood–membrane interactions’ side effects. Zwitterionic structures have shown improved hemocompatibility; however, their complicated synthesis hinders their commercialization. The goal of the study is to achieve fast functionalization for carboxybetaine and sulfobetaine zwitterionic immobilization on PES membranes while comparing the stability and the targeted hemocompatibility. The chemical modification approach is based on an aminolysis reaction. Characterization, computational simulations, and clinical analysis were conducted to study the modified membranes. Atomic force microscopy (AFM) patterns showed a lower mean roughness for carboxybetaine-modified (6.3 nm) and sulfobetaine-modified (7.7 nm) membranes compared to the neat membrane (52.61 nm). The pore size of the membranes was reduced from values above 50 nm for the neat PES to values between 2 and 50 nm for zwitterionized membranes, using Brunauer–Emmett–Teller (BET) analysis. More hydrophilic surfaces led to a growth equilibrium water content (EWC) of nearly 6% for carboxybetaine and 10% for sulfobetaine-modified membranes. Differential scanning calorimetry (DSC) measurements were 12% and 16% stable water for carboxybetaine- and sulfobetaine-modified membranes, respectively. Sulfobetaine membranes showed better compatibility with blood with respect to C5a, IL-1a, and IL-6 biomarkers. Aminolysis-based zwitterionization was found to be suitable for the improvement of hemodialysis membranes. The approach introduced in this paper could be used to modify the current dialysis membranes with minimal change in the production facilities.

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Comparative performance study of polyethersulfone and polysulfone membranes for trypsin isolation from goat pancreas using affinity ultrafiltration

Cited by 11 publications

References 41 publications

Obtainment of a highly concentrated pancreatic serine proteases extract from bovine pancreas by precipitation with polyacrylate

Obtainment of a highly concentrated pancreatic serine proteases extract from bovine pancreas by precipitation with polyacrylate

Membrane Chromatography and Fractionation of Proteins from Whey—A Review

Aminolysis-Based Zwitterionic Immobilization on Polyethersulfone Membranes for Enhanced Hemocompatibility: Experimental, Computational, and Ex Vivo Investigations

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