Comparative permeability and diffusion kinetics of cyclosporine A liposomes and propylene glycol solution from human lung tissue into human blood ex vivo

Pharmacokinetics and tissue distribution profile of icariin propylene glycol-liposome intraperitoneal injection in mice

“…As shown in Figure 5a, the biological half‐life of ICA‐PG‐liposomes (2.672 ± 1.538 h) was much longer than that of ICA‐PG‐solution (0.741 ± 0.422 h) in plasma ( P < 0.05). These results were consistent with previous research in vitro and in vivo [32,33] . Trammer et al [32] .…”

Analysis of Fenoterol and Ipratropium Transfer from Human Lung Tissue into Human Plasma Using a Dynamic Dialysis Model

“…As expected, the differences in the release characteristics from the human lung tissue were striking. As previously seen with beclomethasone dipropionate and cyclosporine A [6,7] we found a continuous accumulation of FP in the acceptor chamber during the static dialysis. Still, sink conditions prevailed over the whole experimental period with drug concentrations in the acceptor chamber being significantly less than 10% of that in the donor chamber.…”

“…The diffusion in our model was mainly governed by the tissue adsorption and desorption processes. However, we recently demonstrated that the diffusion kinetics we determined for two different cyclosporine a formulations in our static dialysis model was very consistent with the permeability data obtained with a Calu-3 cell culture model [7].…”

“…Subsequently, we compared the diffusion kinetics of a liposome formulation and a propylene glycol solution of cyclosporine A. The results we obtained with the dialysis model were in excellent agreement with those of a permeability assay using the human bronchial cell line Calu-3 [7]. This suggested that this dialysis model was appropriate for simulation of pulmonary absorption processes and comparative evaluation of different drugs or drug formulations.…”

“…Static dialysis experiments were performed as described in detail previously [6,7]. Briefly, the dialysis unit (designed by our group) consisted of two individual tightly fitting Teflon chambers separated by a dialysis membrane.…”