Comparative pharmacokinetics of catalpol and acteoside in normal and chronic kidney disease rats after oral administration of <i>Rehmannia glutinosa</i> extract

Zhao, Min; Qian, Dawei; Liu, Pei; Shang, Erxin; Jiang, Shu; Guo, Jianming; Su, Shulan; Duan, Jin‐Ao; Du, Lijun; Jiang, Tao

doi:10.1002/bmc.3505

Cited by 27 publications

(12 citation statements)

References 48 publications

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“…Among the four PhGs, the concentration of the second peak of crenatoside was higher than that of the first peak, but the concentration of the second peaks of the other three analytes were lower than those of their first peaks. The peak times (t max ) of acteoside and isoacteoside were at 0.3 ± 0.1 h and 0.4 ± 0.2 h (Table 7), respectively, which were consistent with previous studies [26,28,29,30]. The t max of martynoside and crenatoside were at 3.1 ± 3.6 h and 6.8 ± 1.1 h, respectively, which were longer than those of acteoside and isoacteoside.…”

Section: Resultssupporting

confidence: 92%

“…The first concentration peaks of all the PhGs appeared at about 0.5 h, and then, reached the second peaks at approximately 6 h in rat plasma. Interestingly, a literature survey indicated that the concentration-time curve features of acteoside and isoacteoside have been reported as double peaks within 1 h in rat plasma after intragastric administration of individual components [25,26], whereas, they have been reported as single peaks [27,28] or double peaks [26,29] within 2 h when administrated medicinal plant extracts. In our study, the relatively distant double peaks of the PhGs might attribute to multiple reasons, such as the influences of complex compositions in AIH, enterohepatic circulation, multiple absorption sites, and gastric emptying process.…”

Section: Resultsmentioning

confidence: 99%

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Simultaneous Quantification of Four Phenylethanoid Glycosides in Rat Plasma by UPLC-MS/MS and Its Application to a Pharmacokinetic Study of Acanthus Ilicifolius Herb

et al. 2019

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Acanthus ilicifolius herb (AIH), the dry plant of Acanthus ilicifolius L., has long been used as a folk medicine for treating acute and chronic hepatitis. Phenylethanoid glycosides (PhGs) are one family of the main components in AIH with hepatoprotective, antioxidant, and anti-inflammatory activities. In this study, the pharmacokinetics of AIH was investigated preliminarily by ultra-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UPLC-MS/MS). A simultaneously quantitative determination method for four PhGs (acteoside, isoacteoside, martynoside, and crenatoside) in rat plasma was first established by UPLC-MS/MS. These four PhGs were separated with an ACQUITY UPLC BEH C18 column (2.1 × 50 mm, 1.7 μm) by gradient elution (mobile phase: MeCN and 0.1% formic acid in water, 0.4 mL/min). The mass spectrometry detection was performed using negative electrospray ionization (ESI−) in multiple reaction monitoring (MRM) mode. By the established method, the preliminary pharmacokinetics of AIH was elucidated using the kinetic parameters of the four PhGs in rat plasma after intragastric administration of AIH ethanol extract. All four PhGs showed double peaks on concentration-time curves, approximately at 0.5 h and 6 h, respectively. Their elimination half-lives (t1/2) were different, ranging from 3.42 h to 8.99 h, although they shared similar molecular structures. This work may provide a basis for the elucidation of the pharmacokinetic characteristics of bioactive components from AIH.

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Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 99%

Simultaneous Quantification of Four Phenylethanoid Glycosides in Rat Plasma by UPLC-MS/MS and Its Application to a Pharmacokinetic Study of Acanthus Ilicifolius Herb

et al. 2019

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“…Despite the fact that catalpol can be transformed by intestinal bacteria, due to its glucoside structure (Tao et al, 2016), a high plasma exposure in normal rats can be reached with a C max of 2.14 μg•ml −1 (equal to~5.9 μM; Zhao et al, 2015). When rats with chronic kidney disease were given catalpol, the C max increased to 7.94 μg•ml −1 (Zhao et al, 2015), which suggests that the kidney is the major elimination pathway for catalpol. Moreover, catalpol showed the highest distribution in rat kidney compared with other tissues (Xue et al, 2015), which implies that the tissue-specific enrichment of catalpol favours its renal protective functions.…”

Section: Discussionmentioning

confidence: 99%

Catalpol alleviates adriamycin‐induced nephropathy by activating the SIRT1 signalling pathway in vivo and in vitro

Zhang

Meng

et al. 2019

British J Pharmacology

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Background and Purpose: Catalpol, a water-soluble active ingredient isolated from Rehmannia glutinosa, exhibits multiple pharmacological activities. However, the mechanism(s) underlying protection against renal injury by catalpol remains unknown. Experimental Approach: Adriamycin-induced kidney injury models associated with podocyte damage were employed to investigate the nephroprotective effects of catalpol. In vivo, TUNEL and haematoxylin-eosin staining was used to evaluate the effect of catalpol on kidney injury in mice. In vitro, effects of catalpol on podocyte damage induced by adriamycin was determined by ELISA kit, flow cytometry, Hoechst 33342, and TUNEL staining. The mechanism was investigated by siRNA, EX527, and docking simulations. Key Results: In vivo, catalpol treatment significantly improved adriamycin-induced kidney pathological changes and decreased the number of apoptotic cells. In vitro, catalpol markedly decreased the intracellular accumulation of adriamycin and reduced the calcium ion level in podocytes and then attenuated apoptosis. Importantly, the regulatory effects of catalpol on sirtuin 1 (SIRT1), multidrug resistance-associated protein 2 (MRP2), and the TRPC6 channel were mostly abolished after incubation with SIRT1 siRNA or the SIRT1-specific inhibitor EX527. Furthermore, docking simulations showed that catalpol efficiently oriented itself in the active site of SIRT1, indicating a higher total binding affinity score than that of other SIRT1 activators, such as resveratrol, SRT2104, and quercetin. Conclusion and Implications: Taken together, our results suggest that catalpol exhibits strong protective effects against adriamycin-induced nephropathy by inducing SIRT1-mediated inhibition of TRPC6 expression and enhancing MRP2 expression. 1 | INTRODUCTION The anthracycline antibiotic adriamycin, a broad-spectrum anti-tumour drug, has been widely used to treat various cancers (Rivankar, 2014). The toxic side effect of adriamycin, mainly associated with cardiotoxicity (Octavia et al., 2012), limits its clinical applications. It has been noted that nephrotoxicity can be induced by adriamycin in rodents but rarely occurs in humans. Anthracycline-induced chronic kidney damage was reported early in 1970 (Sternberg, 1970) and since then animal models of adriamycin-induced kidney injury have been widely established (Bucciarelli, Binazzi, Santori, & Vespasiani, 1976; Chen et al., 2015). It has been reported that the adriamycin-induced classic nephrotoxicity model is very similar to human progressive chronic renal disease (Ajith, Aswathy, & Hema, 2008). Subsequently, Jiangnan Zhang and Ran Bi should be considered joint first author.

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“…Acteoside (ACT), the main component of Rehmannia glutinosa , has a wide range of effects, including regulation of immune and inflammatory responses [ 3 ]. Previous researches demonstrated that acteoside could improve urinary protein excretion and decrease the incidence of mesangial expansion [ 4 , 5 ]. This makes acteoside a possible therapy of IgAN.…”

Section: Introductionmentioning

confidence: 99%

Acteoside relieves mesangial cell injury by regulating Th22 cell chemotaxis and proliferation in IgA nephropathy

Gan

Zhu

et al. 2018

Renal Failure

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The existing therapies of IgA nephropathy are unsatisfying. Acteoside, the main component of Rehmannia glutinosa with anti-inflammatory and anti-immune effects, can improve urinary protein excretion and immune disorder. Th22 cell is involved in IgA nephropathy progression. This study was determined to explore the effect of acteoside on mesangial injury underlying Th22 cell disorder in IgA nephropathy. Serum Th22 cells and urine total protein of patients with IgA nephropathy were measured before and after six months treatment of Rehmannia glutinosa acteoside or valsartan. Chemotactic assay and co-culture assay were performed to investigate the effect of acteoside on Th22 cell chemotaxis and differentiation. The expression of CCL20, CCL22 and CCL27 were analyzed. To explore the effect of acteoside on mesangial cell injury induced by inflammation, IL-1, IL-6, TNF-α and TGF-β1 were tested. Results showed that the proteinuria and Th22 lymphocytosis of patients with IgA nephropathy significantly improved after combination treatment of Rehmannia glutinosa acteoside and valsartan, compared with valsartan monotherapy. In vitro study further demonstrated that acteoside inhibit Th22 cell chemotaxis by suppressing the production of Th22 cell attractive chemokines, i.e., CCL20, CCL22 and CCL27. In addition, acteoside inhibited the Th22 cell proliferation. Co-culture assay proved that acteoside could relieve the overexpression of pro-inflammatory cytokines, and prevent the synthesis of TGF-β1. TGF-β1 level in mesangial cells was positively correlated with the Th22 cell. This research demonstrated that acteoside can alleviate mesangial cell inflammatory injury by modulating Th22 lymphocytes chemotaxis and proliferation.

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Comparative pharmacokinetics of catalpol and acteoside in normal and chronic kidney disease rats after oral administration of Rehmannia glutinosa extract

Cited by 27 publications

References 48 publications

Simultaneous Quantification of Four Phenylethanoid Glycosides in Rat Plasma by UPLC-MS/MS and Its Application to a Pharmacokinetic Study of Acanthus Ilicifolius Herb

Simultaneous Quantification of Four Phenylethanoid Glycosides in Rat Plasma by UPLC-MS/MS and Its Application to a Pharmacokinetic Study of Acanthus Ilicifolius Herb

Catalpol alleviates adriamycin‐induced nephropathy by activating the SIRT1 signalling pathway in vivo and in vitro

Acteoside relieves mesangial cell injury by regulating Th22 cell chemotaxis and proliferation in IgA nephropathy

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