Comparative pharmacological actions of bradykinin and related kinins of larger molecular weights

Reis, Marina Lemos dos; Okino, Liyoko; Silva, M.Rocha e

doi:10.1016/0006-2952(71)90096-7

Cited by 40 publications

(16 citation statements)

References 10 publications

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“…The series of bradykinin derivatives, of increasing molecular weights, L-Bk, ML-Bk, GAML-Bk and Bk itself, have already been studied in relation to their relative potencies upon smooth muscle structures (guinea-pig ileum, rat uterus and rat duodenum), and on rat blood pressure and vascular permeability (Reis et al, 1971; Rocha e Silva et al, 1972;Rocha e Silva, 1971). As regards their action upon smooth muscle structures, they showed decreasing potencies, as the amino acid chain at the NH2 terminal group of Bk was increased, from Bk to GAML-Bk.…”

Section: Discussionmentioning

confidence: 99%

“…The peptides used in the experiments described below were the same as those used in previous experiments in this laboratory (Reis et al, 1971 ; Rocha e : bradykinin (Bk); lysyl-bradykinin (L-Bk); methionyl-lysyl-bradykinin (ML-Bk) and glycyl-arginyl-methionyl-lysyl-bradykinin (GAML-Bk). As shown in previous experiments, their effects decrease with increasing molecular weight when acting on the guinea-pig ileum, the rat uterus and the rat duodenum (Bk>L-Bk> ML-Bk>GAML-Bk) but increase when tested on rat blood pressure and the vascular permeability of the rat's skin (GAML-Bk>ML-Bk>L-Bk>Bk).…”

Section: Synthetic Peptides Related To Bradykininmentioning

confidence: 99%

“…Since the four peptides used in the present experiments have already been assayed upon several other preparations (guinea-pig ileum, rat uterus, duodenum, rat blood pressure and vascular permeability) by Reis et al (1971) and Rocha e Silva (1971), a comparison has been made of the data presented by these authors with the results described in the present paper, to ascertain whether the relative responses of the ventricular lining were similar to those of the smooth muscle preparations or to those of the blood vessels in producing changes in blood pressure and vascular permeability. Figure 5 gives the dose-response curves of the four peptides, taking the 'areas ' of increase in the threshold time curves, for two doses of Bk, L-Bk, ML-Bk and GAML-Bk.…”

Section: Discrimination Coefficients Of the Four Kinins In Different mentioning

confidence: 99%

“…Table 2 shows the same parameters in relation to the increase in vascular permeability and the antinociceptive effect. It can be seen that the activities of the four peptides run in the opposite direction, 235-550 * All data were corrected for molar concentrations; t Data from Reis et al, (1971). since the effect on vascular permeability increases 10 to 20 fold when the larger peptides are used, although the analgesic effect, as shown above, decreases conspicuously to 1/17 for ML-Bk and to 1/21 for GAML-Bk. The discrimination indexes thus rise to the highly significant values of 170 for ML-Bk, and 235-500 for GAML-Bk.…”

Section: Discrimination Coefficients Of the Four Kinins In Different mentioning

confidence: 99%

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Antinociceptive action of bradykinin and related kinins of larger molecular weights by the intraventricular route

Ribeiro¹,

Silva²

1973

British J Pharmacology

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Summary1. It was shown previously that bradykinin (Bk) when given intraventricularly increases the threshold of electrical stimulation of dental pulp in the rabbit. 2. Bradykinin derivatives with increasing molecular weights (lysyl-Bk (L-Bk), methionyl-lysyl-Bk (ML-Bk) and glycyl-arginyl-methionyl-lysyl-Bk (GAMLBk)) were found to produce effects of decreasing intensity on the threshold of electrical stimulation according to the following scale: Bk (1-00)>L-Bk (0-28)>ML-Bk (0060)>GAML-Bk (0 047).3. The four peptides had similar relative activities on the guinea-pig ileum but an inverse relationship in their effects on vascular permeability and rat blood pressure. 4. The discrimination index, increase in vascular permeability/antinociceptive effect rose to values of the order of 170 to 550, taking Bk as the reference peptide (potency=100). 5. We conclude that the increase in threshold of electrical stimulation could not be due to an increase in vascular permeability or decrease of blood pressure. IntroductionCentral effects of bradykinin given by the intraventricular route have been reported in previous papers from this laboratory (Corrado, Ramos & Rocha e Silva,

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Section: Discussionmentioning

confidence: 99%

Section: Synthetic Peptides Related To Bradykininmentioning

confidence: 99%

Section: Discrimination Coefficients Of the Four Kinins In Different mentioning

confidence: 99%

Section: Discrimination Coefficients Of the Four Kinins In Different mentioning

confidence: 99%

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Antinociceptive action of bradykinin and related kinins of larger molecular weights by the intraventricular route

Ribeiro¹,

Silva²

1973

British J Pharmacology

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“…Há outros corantes, além do azul de Evans, que se ligam à albumina, como o azul Celeste de Niágara, o azul Celeste de Pontamina 6BX e o azul de Tripan (2,(10)(11)(12)(13)(14)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29) . O azul de Evans é um corante capaz de ser mensurável por meio de espectrofotometria (11)(12) .…”

Section: Introductionunclassified

Avaliação espectrofotométrica do azul de Evans na reação inflamatória da córnea: estudo experimental em coelhos

Gehlen¹,

Moreira²,

Moreira³

et al. 2004

Arq. Bras. Oftalmol.

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Avaliação espectrofotométrica do azul de Evans na reação inflamatória da córnea: estudo experimental em coelhosObjetivo: Constatar a presença do azul de Evans na córnea normal estipulando o período de tempo de concentração máxima do corante após inoculação endovenosa e estudar a permeabilidade vascular em modelo animal da inflamação corneal induzida por queimadura química por meio de injeção de azul de Evans. Métodos: Cinquenta coelhos foram divididos em 3 grupos: Grupo I (25 animais): injetou-se 20 mg/kg de azul de Evans e os animais foram sacrificados após 8, 10, 12, 14 e 16 horas. Retirou-se a córnea e quantificou-se o corante por meio de micrométodo espectrofotométrico. Grupo II: em 15 animais injetou-se o corante e, após 10 horas, fragmentouse centralmente o tecido com trépanos de 6, 8 e 10 mm. Procedeu-se à extração do azul de Evans da mesma forma que no grupo I. Grupo III: induziu-se queimadura na córnea do olho direito de 10 animais com NaOH a 1 N. Cinco dias após o procedimento, os animais foram sacrificados, sendo que, 10 horas antes do sacrifício, foi inoculado o azul de Evans para que posteriormente se pudesse quantificá-lo. A córnea esquerda serviu como controle. Resultados: No grupo I, a média da concentração do azul de Evans às 10 h. foi de 15,28 ± 0,09 µg/mg. No grupo II, as médias das concentrações do corante foram: 6 mm: 0,93 ± 0,01µg/mg; 8 mm: 1,20 ± 0,06 µg/mg; 10 mm: 1,32 ± 0,05 µg/mg. No grupo III, as médias das concentrações do azul de Evans foram: olho direito (queimadura): 23,74 ± 2,64 µg/mg e olho esquerdo (controle): 16,71 ± 2,04 µg/mg. Conclusões: Quantificou-se o azul de Evans pela primeira vez na córnea de coelhos e constatou-se que, após 10 horas de inoculação endovenosa, o corante atingiu seu pico de concentração no tecido. Concluiu-se que o azul de Evans serve como bom método de quantificação da permeabilidade vascular alterada na córnea de coelhos. INTRODUÇÃODesde 1943, com os primeiros estudos de Rawson, o azul de Evans tem sido amplamente utilizado em diversos trabalhos experimentais. Este corante apresenta algumas características como inocuidade (1)(2) , alta solubilidade em água e afinidade pela albumina (3)(4)(5) . O azul de Evans, quando inoculado via endovenosa, combina-se com a albumina de maneira reversível e dependente de pH (4) .Estudos demonstraram que, após 40 horas da administração endovenosa do azul de Evans em ratos, 90% do corante já havia deixado a circulação, atingindo diversos tecidos do organismo. Depois de 24 horas de inoculação

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Neutrophil Generation of Permeability Enhancing Peptides from Plasma Substrates

Movat

Minta

Saya

et al. 1976

Molecular and Biological Aspects of the Acute Allergic Reaction

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Comparative pharmacological actions of bradykinin and related kinins of larger molecular weights

Cited by 40 publications

References 10 publications

Antinociceptive action of bradykinin and related kinins of larger molecular weights by the intraventricular route

Antinociceptive action of bradykinin and related kinins of larger molecular weights by the intraventricular route

Avaliação espectrofotométrica do azul de Evans na reação inflamatória da córnea: estudo experimental em coelhos

Neutrophil Generation of Permeability Enhancing Peptides from Plasma Substrates

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