Comparative phenotypic characterization of hybrid Shiga toxin-producing / uropathogenic Escherichia coli, canonical uropathogenic and Shiga toxin-producing Escherichia coli

Selasi, Gati Noble; Temme, Imke Johanna; Middendorf-Bauchart, Barbara; Kehl, Alexander; Dobrindt, Ulrich; Mellmann, Alexander

doi:10.1016/j.ijmm.2021.151533

Cited by 9 publications

(8 citation statements)

References 42 publications

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“…We also investigated the other IPEC pathotypes among UPEC isolates, but none of them were found. Although the detection of UPEC/STEC strains has been reported in some studies [1,7,10,15], we could not find reports regarding the presence of UPEC/ ETEC or UPEC/EIEC strains and it appears that these strains are not currently circulating.…”

Section: Discussioncontrasting

confidence: 71%

“…Therefore, with respect to the genetic exchanges between bacteria through HGT, this makes it difficult to classify pathogenic E. coli isolates into distinct pathotypes [7].…”

Section: Discussionmentioning

confidence: 99%

“…Besides these canonical DEC and ExPEC pathogroups, another group of pathogenic E. coli isolates have recently been identified, which contain VG markers of two or more different pathogroups [7]. The ‘hetero-pathogenic’ or ‘heteropathogen’ group refers to strains which contain virulence markers of two or more DEC pathotypes.…”

Section: Introductionmentioning

confidence: 99%

“…However, 'hybrid-pathogenic' or 'hybrid-pathogen' is applied to strains that harbour both ExPEC-and DEC-defining VGs or, alternatively, encode DEC-defining VGs, but are isolated from extraintestinal infections [3]. One of the most important hybrids reported has been the EAEC/STEC (O104:H4), which caused the major haemolytic uraemic syndrome (HUS) outbreak in Europe in 2011 [7][8][9]. In Denmark, a clonal EAEC was isolated from a cluster of UTIs [10,11].…”

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confidence: 99%

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Detection and characterization of hybrid uropathogenic Escherichia coli strains among E. coli isolates causing community-acquired urinary tract infection

Yousefipour

Rezatofighi

Ardakani

2023

Journal of Medical Microbiology

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Introduction. The main aetiological agent of urinary tract infection (UTI) is Escherichia coli , categorized as uropathogenic E. coli (UPEC). The genome of UPEC shows a high degree of plasticity, which leads to the emergence of ‘intermediary strains’ with different traits from the parental pathotypes. Gap Statement/Aim. We aimed to assess the frequency and types of the hybrid UPEC among isolates causing UTI and characterize virulence properties of these hybrid isolates molecularly and phenotypically. Methodology. After detection of intestinal pathogenic E. coli (IPEC) virulence markers among 200 UPEC isolates, they were assessed for the presence of 40 virulence genes (VGs) of extraintestinal, uropathogenic and diarrhoeagenic E. coli , phylogenetic group typing, phenotypic traits including biofilm formation, adherence and invasion to HeLa cells, haemolysis activity and antimicrobial resistance. Results. The analysis showed 21 (10.5 %) UPEC isolates carried enteroaggregative E. coli (EAEC) and enteropathogenic E. coli (EPEC) virulence markers. Twenty isolates carried the aggR (EAEC) and one the eae and escV genes (EPEC), which were classified as hybrid strains. The most commonly identified genes were fimH (71.5 %), fyuA (66.7 %), iutA (62 %), chuA (57.1) and traT (47.6 %). Biofilm production, adhesion and invasion were found among 17 (81), 18 (85.7) and 11 (52.4 %) hybrids, respectively. Investigation of the genetic characteristics, phylogenetic group and virulence profile of the detected hybrids revealed that they have genetic diversity and do not belong to a particular clonal lineage. Conclusion. The present study reveals that some UPEC may carry virulence markers of IPEC pathotypes. EAEC and EPEC seem to have a greater tendency to form hybrids and cause UTI. Further studies are needed to elucidate what factors contributed to survival in the urinary tract system and facilitate infection and whether these combinations lead to an increase in pathogenicity or not.

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Section: Discussioncontrasting

confidence: 71%

“…Therefore, with respect to the genetic exchanges between bacteria through HGT, this makes it difficult to classify pathogenic E. coli isolates into distinct pathotypes [7].…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Detection and characterization of hybrid uropathogenic Escherichia coli strains among E. coli isolates causing community-acquired urinary tract infection

Yousefipour

Rezatofighi

Ardakani

2023

Journal of Medical Microbiology

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“…Another example is the STEC/UPEC serotype O2:H6 ST141 strain, a STEC with virulence genes α-hlyA , cnf1 , and clb common to UPEC [ 36 , 37 ]. This hybrid strain can be a melting pot for pathotype conversion of InPEC and ExPEC because it acquired the stx -harboring prophage and ExPEC PAI.…”

Section: Introductionmentioning

confidence: 99%

The Diversity of Escherichia coli Pathotypes and Vaccination Strategies against This Versatile Bacterial Pathogen

2023

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Escherichia coli (E. coli) is a gram-negative bacillus and resident of the normal intestinal microbiota. However, some E. coli strains can cause diseases in humans, other mammals and birds ranging from intestinal infections, for example, diarrhea and dysentery, to extraintestinal infections, such as urinary tract infections, respiratory tract infections, meningitis, and sepsis. In terms of morbidity and mortality, pathogenic E. coli has a great impact on public health, with an economic cost of several billion dollars annually worldwide. Antibiotics are not usually used as first-line treatment for diarrheal illness caused by E. coli and in the case of bloody diarrhea, antibiotics are avoided due to the increased risk of hemolytic uremic syndrome. On the other hand, extraintestinal infections are treated with various antibiotics depending on the site of infection and susceptibility testing. Several alarming papers concerning the rising antibiotic resistance rates in E. coli strains have been published. The silent pandemic of multidrug-resistant bacteria including pathogenic E. coli that have become more difficult to treat favored prophylactic approaches such as E. coli vaccines. This review provides an overview of the pathogenesis of different pathotypes of E. coli, the virulence factors involved and updates on the major aspects of vaccine development against different E. coli pathotypes.

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Emergence of Hybrid Escherichia coli Strains

Gomes

Santos

Hernandes

et al. 2023

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Comparative phenotypic characterization of hybrid Shiga toxin-producing / uropathogenic Escherichia coli, canonical uropathogenic and Shiga toxin-producing Escherichia coli

Cited by 9 publications

References 42 publications

Detection and characterization of hybrid uropathogenic Escherichia coli strains among E. coli isolates causing community-acquired urinary tract infection

Detection and characterization of hybrid uropathogenic Escherichia coli strains among E. coli isolates causing community-acquired urinary tract infection

The Diversity of Escherichia coli Pathotypes and Vaccination Strategies against This Versatile Bacterial Pathogen

Emergence of Hybrid Escherichia coli Strains

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