Comparative phylogenetic relationships and genetic structure of the caterpillar fungus Ophiocordyceps sinensis and its host insects inferred from multiple gene sequences

Quan, Qingmei; Wang, Qingxia; Zhou, Xueli; Li, Shan; Yang, Xiao; Zhu, Yazhi; Zhou, Changyong

doi:10.1007/s12275-014-3391-y

Cited by 22 publications

(22 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, mitochondrial COI and Cytb sequences in host insect of Bhutanese Cordyceps were 658 bp and 433 bp, and GC contents were 29.18% and 22.86%, respectively. Both mitochondrial COI and Cytb sequences suggested that species of host insect of Bhutanese Cordyceps belonged to Hepialidae sp (99% homologous)20, which was in accordance with those of C. sinensis 32122.…”

Section: Resultssupporting

confidence: 59%

Cordyceps collected from Bhutan, an appropriate alternative of Cordyceps sinensis

Zheng

et al. 2016

Sci Rep

View full text Add to dashboard Cite

Natural Cordyceps collected in Bhutan has been widely used as natural Cordyceps sinensis, an official species of Cordyceps used as Chinese medicines, around the world in recent years. However, whether Cordyceps from Bhutan could be really used as natural C. sinensis remains unknown. Therefore, DNA sequence, bioactive components including nucleosides and polysaccharides in twelve batches of Cordyceps from Bhutan were firstly investigated, and compared with natural C. sinensis. Results showed that the fungus of Cordyceps from Bhutan was C. sinensis and the host insect belonged to Hepialidae sp. In addition, nucleosides and their bases such as guanine, guanosine, hypoxanthine, uridine, inosine, thymidine, adenine, and adenosine, as well as compositional monosaccharides, partial acid or enzymatic hydrolysates, molecular weights and contents of polysaccharides in Cordyceps from Bhutan were all similar to those of natural C. sinensis. All data suggest that Cordyceps from Bhutan is a rational alternative of natural C. sinensis, which is beneficial for the improvement of their performance in health and medicinal food areas.

show abstract

Section: Resultssupporting

confidence: 59%

Cordyceps collected from Bhutan, an appropriate alternative of Cordyceps sinensis

Zheng

et al. 2016

Sci Rep

View full text Add to dashboard Cite

show abstract

“…The mitochondrial COI sequence is not only more reliable than either cytochrome oxidase subunit II (COII) or cytochrome b (Cyt b ), but in combined analysis of COI+COII+Cyt b is also more representative of the phylogenetic relationships and geographical distribution patterns of host insects from different O. sinensis populations [25]. The host insect COI sequences of all 400 samples were sequenced.…”

Section: Methodsmentioning

confidence: 99%

Genetic Diversity and Distribution Patterns of Host Insects of Caterpillar Fungus Ophiocordyceps sinensis in the Qinghai-Tibet Plateau

Quan

Wang

et al. 2014

PLoS ONE

Self Cite

View full text Add to dashboard Cite

The caterpillar fungus Ophiocordyceps sinensis is one of the most valuable medicinal fungi in the world, and it requires host insects in family Hepialidae (Lepidoptera) to complete its life cycle. However, the genetic diversity and phylogeographic structures of the host insects remain to be explored. We analyzed the genetic diversity and temporal and spatial distribution patterns of genetic variation of the host insects throughout the O. sinensis distribution. Abundant haplotype and nucleotide diversity mainly existed in the areas of Nyingchi, ShangriLa, and around the edge of the Qinghai-Tibet Plateau, where are considered as the diversity center or micro-refuges of the host insects of O. sinensis. However, there was little genetic variation among host insects from 72.1% of all populations, indicating that the host species composition might be relatively simple in large-scale O. sinensis populations. All host insects are monophyletic except for those from four O. sinensis populations around Qinghai Lake. Significant phylogeographic structure (NST>GST, P<0.05) was revealed for the monophyletic host insects, and the three major phylogenetic groups corresponded with specific geographical areas. The divergence of most host insects was estimated to have occurred at ca. 3.7 Ma, shortly before the rapid uplift of the QTP. The geographical distribution and star-like network of the haplotypes implied that most host insects were derived from the relicts of a once-widespread host that subsequently became fragmented. Neutrality tests, mismatch distribution analysis, and expansion time estimation confirmed that most host insects presented recent demographic expansions that began ca. 0.118 Ma in the late Pleistocene. Therefore, the genetic diversity and distribution of the present-day insects should be attributed to effects of the Qinghai-Tibet Plateau uplift and glacial advance/retreat cycles during the Quaternary ice age. These results provide valuable information to guide the protection and sustainable use of these host insects as well as O. sinensis.

show abstract

“…High commercial value and habitat degradation of natural DCXC has led to overexploitation, which has endangered the species in recent years. The number of natural DCXC populations is extremely limited, and its residential ecological environments are also potentially threatened [9]. To protect DCXC resources and its habitats, an effective approach is to study and develop substitutes represented by endogenetic microorganisms isolated from natural DCXC, based on consideration of its specific bioactive components.…”

Section: Introductionmentioning

confidence: 99%

Pyrosequencing analysis revealed complex endogenetic microorganism community from natural DongChong XiaCao and its microhabitat

et al. 2016

View full text Add to dashboard Cite

BackgroundOphiocordyceps sinensis (DongChong XiaCao (DCXC) in Chinese), a fungal parasite of caterpillars, is a traditional Chinese medicine. Bioactive components isolated from natural DCXC possess a wide range of pharmacological actions. Many efforts have been directed towards isolating the fungi based on culture-dependent methods for investigation of fungal diversity in order to determine the anamorph of natural DCXC and find new medicinal fungi resources, and the results have been varied.ResultsIn the present study, a total of 44,588 bacterial and 51,584 fungal sequences corresponding to 11,694 and 9297 putative operational taxonomic units (OTU) were respectively identified by a Roche/454-based, high throughput sequence analysis of 16S rRNA genes and ITS regions. The main bacterial groups were Proteobacteria, Acidobacteria, Bacteroidetes, Actinobacteria and Firmicutes, while the Ascomycota, Basidiomycota and Zygomycota were the main fungal phyla. Proteobacteria presented 68.4, 49.5, 38.9 and 35.6 % of all bacteria in the sclerotia, stromata, external mycelial cortices and soil, respectively. As the main fungi phyla, Ascomycota presented 21.0, 45.6 26.4 and 59.3 % in the sclerotia, stromata, external mycelial cortices and soil, respectively. Bacterial and fungal communities were more diverse in the environmental sample than in the natural DCXC sample. Microbial communities were obviously distinct in each sample. Several novel unclassifiable bacterial (10.41 %) and fungal (37.92 %) species were also detected.ConclusionsThis study revealed an abundant endogenetic fungal and bacterial resources and a variety of genetic information in natural DCXC by high-throughput 454 sequencing technology. Microorganism that had been discovered in natural DCXC will provide sources for screening the new bioactive metabolites and its biotechnological application.Electronic supplementary materialThe online version of this article (doi:10.1186/s12866-016-0813-5) contains supplementary material, which is available to authorized users.

show abstract

Comparative phylogenetic relationships and genetic structure of the caterpillar fungus Ophiocordyceps sinensis and its host insects inferred from multiple gene sequences

Cited by 22 publications

References 29 publications

Cordyceps collected from Bhutan, an appropriate alternative of Cordyceps sinensis

Cordyceps collected from Bhutan, an appropriate alternative of Cordyceps sinensis

Genetic Diversity and Distribution Patterns of Host Insects of Caterpillar Fungus Ophiocordyceps sinensis in the Qinghai-Tibet Plateau

Pyrosequencing analysis revealed complex endogenetic microorganism community from natural DongChong XiaCao and its microhabitat

Contact Info

Product

Resources

About