Comparative primary structure analysis of the p30 protein of woolly monkey and gibbon type C viruses

Amino-terminal amino acid sequence of the major structural polypeptides of avian retroviruses: sequence homology between reticuloendotheliosis virus p30 and p30s of mammalian retroviruses.

“…6). REV p30 contains the same proline-leucine-arginine starting sequence that characterizes all mammalian virus p30 polypeptides (10), and in addition shows homology with residues 4 and 5 of FeLV p30. The REV p30 sequence differs from the two mammalian viruses in that it posies an insertion of-six amino acids between residues 14 and 15 of the mammalian sequence (Fig.…”

“…Amino acid sequence analyses of the amino-terminal region of the p30 polypeptides from a variety of mammalian viruses (10) have shown that the amino-terminal sequence is highly conserved, even between viruses that show only weak antigenic crossreactivity. To determine whether a similar structurefunction relationship can be observed in avian type C viruses, we have compared the amino-terminal sequence of p27 from avian sarcoma virus (ASV) B77 and p30 from reticuloendotheliosis virus (strain T).…”

Studies on Proteins of Simian Sarcoma-associated Virus with Different Growth History

“…Pr65 gag is often found in murine leukaemia virus preparations (Lu et al, 1979). One-dimensional peptide maps of p30 were identical in the original virus stock as compared to virus passaged 25 times through H, D and R cells, confirming the conservative nature of SSaV p30 (Oroszlan et al, 1977). The absence of altered peptides in the p30 digests indicates that no recombination has occurred with endogenous retroviral p30 genes that might be present in human cells.…”

“…of gp70, p30 and p15 from N-and B-tropic virus are slightly different and that recombinants can be recognized on the basis of these differences (Schindler et al, 1977). If human cells do contain endogenous retrovirus, then recombination of related genomes with the exogenous SSaV infecting virus might also occur, and if an alteration in the peptide maps of p30 was found this would be highly suggestive of a recombination event, as the p30 of SSaV and the related gibbon ape leukaemia virus (GaLV) have identical peptide maps, demonstrating the conservation of this protein (Oroszlan et al, 1977).…”

Antigen related to mammalian type-C RNA viral p30 proteins is located in renal glomeruli in human systemic lupus erythematosus.

“…The study of the interspecies determinants of purified p30 proteins shows immunological cross-reaction between all species of mammalian type-C viruses tested (11,15,19,20), including murine, feline, endogenous feline (RD-114), and woolly monkey viruses (21), and also distinguishable sets of interspecies determinants that are shared by closely related viruses, murine and feline (21-23), and baboon (endogenous primate) (23, 24), woolly monkey and gibbon ape (infectious primate) (23,25,26), and murine, feline, and infectious primate viruses (21, 23). A high degree of aminoacid sequence homology is found in the p,30 proteins of closely related mammalian type-C viruses (27). The development of assays for type-C viral nucleic acids and proteins advances the search for putative human type-C viruses; recent reports indicate that type-C virus genetic information is expressed in human tissues.…”