Comparative proteomic analysis of grain development in two spring wheat varieties under drought stress

Abstract

Two spring wheat varieties Ningchun 4 and Chinese Spring with good and poor resistance to abiotic stress, respectively, were used to investigate proteomic changes in the developing grains under drought stress by a comparative proteomics approach. A total of 152 protein spots showed at least twofold differences in abundance on two-dimensional electrophoresis (2-DE) maps, of which 28 and 68 protein spots were identified by MALDI-TOF and MALDI-TOF/TOF mass spectrometry, respectively. Of the 96 identified protein spots, six different expression patterns were found and they were involved in stress/defense/detoxification, carbohydrate metabolism, photosynthesis, nitrogen metabolism, storage proteins and some other important functions. Comparative proteomic analysis revealed that under the drought conditions the decreased degree of ascorbate peroxidases was more significant in Chinese Spring than in Ningchun 4 during grain development whereas translationally controlled tumor protein, which was significantly upregulated at 14 DAF, was present in Ningchun 4 and absent in Chinese Spring. The Rubisco large subunit displayed an upregulated expression pattern in Ningchun 4. In addition, two drought-tolerant proteins, triosephosphate isomerase and oxygen-evolving complex showed B and F type expression patterns in Chinese Spring, but D and B types in Ningchun 4, respectively. These differentially expressed proteins might be responsible for the stronger drought resistance of Ningchun 4 compared to Chinese Spring.

Changes in the root proteome of Triticosecale grains germinating under osmotic stress

“…A significant decrease in expression of peroxidase was observed under mild osmotic stress conditions. Reduction of peroxidase accumulation by the action of osmotic stress was observed in other studies (Jiang and Deyholos 2006;Ma et al 2009;Ge et al 2012). Decrease in the abundance of this enzyme in the tissues could reduce the adaptability of the plants to stress as evidenced, for example, in the study of Arabidopsis mutants, deprived of the ability to synthesize ascorbate peroxidase (Koussevitzky et al 2008).…”

“…This enzyme is involved in synthesis of a-glucan, found in the plant's primary reserve material-starch (Hochstenbach et al 1998;Kok-Jacon et al 2003). It was demonstrated that osmotic stress causes changes in the biosynthesis of starch (Ge et al 2012). A reduced demand for carbohydrates in cells may be due to the inhibition of triticale growth under osmotic stress (Brosowska-Arendt and Weidner 2011).…”

Proteomics Analysis of Hepatoprotective Effects for Scoparone Using MALDI-TOF/TOF Mass Spectrometry with Bioinformatics

“…Recently, MS-based proteomics has the potential to identify novel proteins that are related to the action mechanisms of natural products (Barnett et al, 2012;Schrattenholz et al, 2010;Zhang et al, 2013). Various types of biological networks are being constructed, including protein-protein interaction networks, and protein regulatory networks, to help drug discovery (Abu-Asab et al, 2011;Ge et al, 2012).…”

Comparative proteomic analysis of wheat developing grains between Chinese Spring and 1Sl/1B substitution line

“…2-DE was performed according to Ge et al (2011), and 600 μg of proteins was loaded into analytical and preparative gels (pH 3-10, 18 cm, GE Healthcare). The 2-DE experiments of each sample were repeated 2-3 times.…”