Comparative proteomic analysis of male and female plants in Jojoba (Simmondsia chinensis) leaves revealed changes in proteins involved in photosynthesis, metabolism, energy, and biotic and abiotic stresses

Al‐Obaidi, Jameel R.; Rahmad, Norasfaliza; Hanafi, Nursyuhaida Mohd; Halabi, Mohammed Farouq; Al-Soqeer, A.

doi:10.1007/s11738-017-2485-7

Cited by 17 publications

(4 citation statements)

References 45 publications

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“…Jojoba has been extensively studied using molecular markers to investigate the genetic diversity among varieties to improve oil production and to detect the sex of the plant. In these studies, a wide range of molecular markers were employed, including ISSR [28,29], AFLP [30], SCAR [3], SCoT [31], and protein-based biomarkers [32]. The focus of these studies was to enhance jojoba oil production through biodiversity and breeding.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis of Jojoba (Simmondsia chinensis) during Seed Development and Liquid Wax Ester Biosynthesis

Alotaibi

Elseehy

Aljuaid

et al. 2020

Plants

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Jojoba is one of the main two known plant source of natural liquid wax ester for use in various applications, including cosmetics, pharmaceuticals, and biofuel. Due to the lack of transcriptomic and genomic data on lipid biosynthesis and accumulation, molecular marker breeding has been used to improve jojoba oil production and quality. In the current study, the transcriptome of developing jojoba seeds was investigated using the Illunina NovaSeq 6000 system, 100 × 106 paired end reads, an average length of 100 bp, and a sequence depth of 12 Gb per sample. A total of 176,106 unigenes were detected with an average contig length of 201 bp. Gene Ontology (GO) showed that the detected unigenes were distributed in the three GO groups biological processes (BP, 5.53%), cellular component (CC, 6.06%), and molecular functions (MF, 5.88%) and distributed in 67 functional groups. The lipid biosynthesis pathway was established based on the expression of lipid biosynthesis genes, fatty acid (FA) biosynthesis, FA desaturation, FA elongation, fatty alcohol biosynthesis, triacylglycerol (TAG) biosynthesis, phospholipid metabolism, wax ester biosynthesis, and lipid transfer and storage genes. The detection of these categories of genes confirms the presence of an efficient lipid biosynthesis and accumulation system in developing jojoba seeds. The results of this study will significantly enhance the current understanding of wax ester biology in jojoba seeds and open new routes for the improvement of jojoba oil production and quality through biotechnology applications.

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Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis of Jojoba (Simmondsia chinensis) during Seed Development and Liquid Wax Ester Biosynthesis

Alotaibi

Elseehy

Aljuaid

et al. 2020

Plants

View full text Add to dashboard Cite

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“…Protein spots from 2DE gel analysis were manually excised and then subjected to in‐gel trypsin digestion as previously described . Digested proteins were subjected to desaltation and concentrated using Zip‐Tip C18 (ZipTip, Millipore, USA) before MALDI–TOF MS/MS analysis (ultrafleXtreme Bruker Daltonics, Bremen, Germany).…”

Section: Methodsmentioning

confidence: 99%

Proteomic and metabolomic study of wax apple (Syzygium samarangense) fruit during ripening process

et al. 2018

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Wax apple is one of the underutilized fruits that is considered a good source of fibers, vitamins, minerals as well as antioxidants. In this study, a comparative analysis of the developments of wax fruit ripening at the proteomic and metabolomic level was reported. 2D electrophoresis coupled with MALDI-TOF/TOF was used to compare the proteome profile from three developmental stages named immature, young, and mature fruits. In general, the protein expression profile and the identified proteins function were discussed for their potential roles in fruit physiological development and ripening processes. The metabolomic investigation was also performed on the same samples using quadrupole LC-MS (LC-QTOF/MS). Roles of some of the differentially expressed proteins and metabolites are discussed in relation to wax apple ripening during the development. This is the first study investigating the changes in the proteins and metabolites in wax apple at different developmental stages. The information obtained from this research will be helpful in developing biomarkers for breeders and help the plant researchers to avoid wax apple cultivation problems such as fruit cracking.

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“…Genomic DNA sequencing is considered important information which can be used for assessment and improvement of plants [ 81 ]. Recently, it has been revealed that proteins involved in metabolism, energy biotic and abiotic stress exhibit differences with high expression level in male compared to the female plants [ 82 ]. The potential benefit of molecular assessment in the jojoba plant could enable screening of its varieties of high oil quality and quantity, and examine the factors in which they are hereditarily identical.…”

Section: Genetic Diversity and Molecular Biology Approachesmentioning

confidence: 99%

A review on plant importance, biotechnological aspects, and cultivation challenges of jojoba plant

et al. 2017

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Jojoba is considered a promising oil crop and is cultivated for diverse purposes in many countries. The jojoba seed produces unique high-quality oil with a wide range of applications such as medical and industrial-related products. The plant also has potential value in combatting desertification and land degradation in dry and semi-dry areas. Although the plant is known for its high-temperature and high-salinity tolerance growth ability, issues such as its male-biased ratio, relatively late flowering and seed production time hamper the cultivation of this plant. The development of efficient biotechnological platforms for better cultivation and an improved production cycle is a necessity for farmers cultivating the plant. In the last 20 years, many efforts have been made for in vitro cultivation of jojoba by applying different molecular biology techniques. However, there is a lot of work to be done in order to reach satisfactory results that help to overcome cultivation problems. This review presents a historical overview, the medical and industrial importance of the jojoba plant, agronomy aspects and nutrient requirements for the plant’s cultivation, and the role of recent biotechnology and molecular biology findings in jojoba research.

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Comparative proteomic analysis of male and female plants in Jojoba (Simmondsia chinensis) leaves revealed changes in proteins involved in photosynthesis, metabolism, energy, and biotic and abiotic stresses

Cited by 17 publications

References 45 publications

Transcriptome Analysis of Jojoba (Simmondsia chinensis) during Seed Development and Liquid Wax Ester Biosynthesis

Transcriptome Analysis of Jojoba (Simmondsia chinensis) during Seed Development and Liquid Wax Ester Biosynthesis

Proteomic and metabolomic study of wax apple (Syzygium samarangense) fruit during ripening process

A review on plant importance, biotechnological aspects, and cultivation challenges of jojoba plant

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