Comparative proteomic analysis reveals molecular mechanism of seedling roots of different salt tolerant soybean genotypes in responses to salinity stress

Ma, Hongyu; Song, Liru; Huang, Zhigang; Yang, Yan; Wang, Shuang; Wang, Zhankui; Tong, Jianhua; Gu, Wei; Ma, Hao; Xiao, Langtao

doi:10.1016/j.euprot.2014.05.005

Cited by 28 publications

(14 citation statements)

References 41 publications

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“…It has also been proposed by Chi et al (2013) that the redox proteins like PDI undergo redox-dependent structural and functional switching from low to high molecular weight protein complexes under stress, thereby functioning as a chaperone. These findings are supported by Ma et al (2014) who demonstrated that PDI activity was enhanced more in the salt-tolerant soyabean genotype Lee 68 as compared to the salt-sensitive genotype Jackson. Also, Huang et al (2012) in their study on heat-tolerant (Agrostisscabra) and heat-sensitive (Agrostisstolonifera) cultivars of perennial grass showed decline in PDI abundance in the heat-sensitive cultivar upon heat exposure.…”

Section: Changes In Activities Of Asada-halliwell Enzymessupporting

confidence: 81%

Anti-oxidative potential of boiling soluble antioxidant enzymes inAmelioration of drought-induced oxidative stress in tolerant and sensitive cultivars of Triticum aestivum

Rakhra¹,

Sharma²,

Singh

2015

J. Crop Sci. Biotechnol.

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Drought is one of the most important abiotic stress factors that limit plant growth and productivity but studies documenting how drought stress regulates boiling soluble antioxidants in cultivars differing in drought tolerance has not been critically evaluated. In this study, we analysed the indices of oxidative stress {H2O2, O2 .-, Membrane Injury Index (MII), Membrane Stability Index (MSI) and lipid peroxidation (MDA)} and activities of ROS-scavenging boiling soluble antioxidant enzymes in caryopses of sensitive and tolerant cultivars of wheat at different stages of development. Water Content (WC) recorded a decline in both the sensitive cvs. PBW 621 and 343 while in tolerant cvs. PBW 175 and 527, it remained unresponsive to drought conditions at 35 DPA. Oxidative stress indicators increased in a cultivar genotype and developmental stage dependent manner under stress. MII increased in all the cultivars under stress conditions in a stage specific manner. Basal activities of various boiling soluble antioxidants like BsMDAR, BsCAT, BsAPX, and BsGR were significantly higher in caryopses of tolerant cvs. PBW 527 and 175 as compared to sensitive cvs.PBW 343 and 621. Activities of BsMDAR, BsGST, BsPDI, BsGR, BsSOD, BsGPX, and BsAPX were induced or increased in the tolerant cvs.PBW 527 and 175 under drought stress while nil or decreased activities were detected in the sensitive cvs. PBW 343 and 621 at different developmental stages. Based upon our findings, it can be inferred that tolerant cultivars may have more biochemical capacity to perform biological antioxidative reactions to combat drought-induced oxidative stress.

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Section: Changes In Activities Of Asada-halliwell Enzymessupporting

confidence: 81%

Anti-oxidative potential of boiling soluble antioxidant enzymes inAmelioration of drought-induced oxidative stress in tolerant and sensitive cultivars of Triticum aestivum

Rakhra¹,

Sharma²,

Singh

2015

J. Crop Sci. Biotechnol.

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“…In agreement, in this study a G‐protein, a phosphatase methylesterase 1 and two calcium‐binding EF‐hand proteins (CaEF) were identified (Supporting Information Table S4). Almost similar result was obtained before when the roots of a salt tolerant soybean cultivar were exposed to moderate salinity (100 mmol/L) . When transferred into tobacco, the CaEF gene considerably increases its salt tolerance .…”

Section: Discussionsupporting

confidence: 82%

“…There is a general acceptance that under salt stress, many stress‐responsive signals (e.g., Ca 2+ , ROS and ethylene) are perceived by their receptors/sensors and transduced through kinase‐mediated protein phosphorylation and/or G‐proteins to regulate the corresponding signaling and metabolic pathways . In agreement, in this study a G‐protein, a phosphatase methylesterase 1 and two calcium‐binding EF‐hand proteins (CaEF) were identified (Supporting Information Table S4).…”

Section: Discussionsupporting

confidence: 75%

Shotgun proteomic analysis of soybean embryonic axes during germination under salt stress

et al. 2016

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Seed imbibition and radicle emergence are generally less affected by salinity in soybean than in other crop plants. In order to unveil the mechanisms underlying this remarkable salt tolerance of soybean at seed germination, a comparative label-free shotgun proteomic analysis of embryonic axes exposed to salinity during germination sensu stricto (GSS) was conducted. The results revealed that the application of 100 and 200 mmol/L NaCl stress was accompanied by significant changes (>2-fold, P<0.05) of 97 and 75 proteins, respectively. Most of these salt-responsive proteins (70%) were classified into three major functional categories: disease/defense response, protein destination and storage and primary metabolism. The involvement of these proteins in salt tolerance of soybean was discussed, and some of them were suggested to be potential salt-tolerant proteins. Furthermore, our results suggest that the cross-protection against aldehydes, oxidative as well as osmotic stress, is the major adaptive response to salinity in soybean.

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“…Protein spots were rehydrated in digestion solution with sequencing grade modified trypsin as described previously [85]. After digestion, the tryptic peptides were extracted, lyophilized and stored at −80 °C.…”

Section: Methodsmentioning

confidence: 99%

Comparative Proteomic and Physiological Analysis Reveals the Variation Mechanisms of Leaf Coloration and Carbon Fixation in a Xantha Mutant of Ginkgo biloba L.

Liu

Wang

et al. 2016

IJMS

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Yellow-green leaf mutants are common in higher plants, and these non-lethal chlorophyll-deficient mutants are ideal materials for research on photosynthesis and plant development. A novel xantha mutant of Ginkgo biloba displaying yellow-colour leaves (YL) and green-colour leaves (GL) was identified in this study. The chlorophyll content of YL was remarkably lower than that in GL. The chloroplast ultrastructure revealed that YL had less dense thylakoid lamellae, a looser structure and fewer starch grains than GL. Analysis of the photosynthetic characteristics revealed that YL had decreased photosynthetic activity with significantly high nonphotochemical quenching. To explain these phenomena, we analysed the proteomic differences in leaves and chloroplasts between YL and GL of ginkgo using two-dimensional gel electrophoresis (2-DE) coupled with MALDI-TOF/TOF MS. In total, 89 differential proteins were successfully identified, 82 of which were assigned functions in nine metabolic pathways and cellular processes. Among them, proteins involved in photosynthesis, carbon fixation in photosynthetic organisms, carbohydrate/energy metabolism, amino acid metabolism, and protein metabolism were greatly enriched, indicating a good correlation between differentially accumulated proteins and physiological changes in leaves. The identifications of these differentially accumulated proteins indicates the presence of a specific different metabolic network in YL and suggests that YL possess slower chloroplast development, weaker photosynthesis, and a less abundant energy supply than GL. These studies provide insights into the mechanism of molecular regulation of leaf colour variation in YL mutants.

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Comparative proteomic analysis reveals molecular mechanism of seedling roots of different salt tolerant soybean genotypes in responses to salinity stress

Cited by 28 publications

References 41 publications

Anti-oxidative potential of boiling soluble antioxidant enzymes inAmelioration of drought-induced oxidative stress in tolerant and sensitive cultivars of Triticum aestivum

Anti-oxidative potential of boiling soluble antioxidant enzymes inAmelioration of drought-induced oxidative stress in tolerant and sensitive cultivars of Triticum aestivum

Shotgun proteomic analysis of soybean embryonic axes during germination under salt stress

Comparative Proteomic and Physiological Analysis Reveals the Variation Mechanisms of Leaf Coloration and Carbon Fixation in a Xantha Mutant of Ginkgo biloba L.

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