Comparative proteomic approach to identify proteins involved in flooding combined with salinity stress in soybean

Abstract

Salinity together with waterlogging or flooding, a condition that occurs frequently in the field, can cause severe damage to crops. Combined flooding and salinity decreases the growth and survival of plants more than either stress alone. We report here the first proteomic analysis to investigate the global effects of saline flooding on multiple metabolic pathways. Soybean seedlings at the emergence (VE) stage were treated with 100 mM NaCl and flooded with water or 100 mM sodium chloride solution for 2 days. Proteins were extracted from hypocotyl and root samples and analyzed by two-dimensional gel electrophoresis followed by MALDI-TOF, MALDI-TOF/TOF mass spectrometry or immunoblotting. A total of 43 reproducibly resolved, differentially expressed protein spots visualized by Coomassie brilliant blue staining were identified by MALDI-TOF MS. Identities of several proteins were also validated by MS/MS analysis or immunoblot analysis. Twenty-nine proteins were upregulated, eight proteins were downregulated and six spots were newly induced. The identified proteins include well-known salt and flooding induced proteins as well as novel proteins expressed by the salinity-flooding combined stress. The comparative analysis identified changes at the proteome level that are both specific and part of a common or shared response. The identification of such differentially expressed proteins provides new targets for future studies that will allow assessment of their physiological roles and significance in the response of glycophytes to a combination of flooding and salinity.

Putative bacterial volatile-mediated growth in soybean (Glycine max L. Merrill) and expression of induced proteins under salt stress

“…Soybean VSP protein was reported with increased expression in drought, salinity and flooding stress (Alam et al . ; Mohammadi et al . ).…”

Salinity (NaCl), Waterlogging, and their Combined Effects on Germination and Seedling Growth of Four Turfgrass Species

“…High soil salinity reduces soil water potential, inhibiting plant water absorption (12). High Na + uptake in salt‐exposed plants interferes with K + uptake, enzyme activities, and metabolic processes (2,3). Therefore, reduced turfgrass quality and sub‐par playing conditions are common problems associated with salt‐affected turfgrass areas (12).…”

Salt‐stress induced proteomic changes of two contrasting alfalfa cultivars during germination stage

“…Alcohol dehydrogenase (>3‐fold, P < 0.01) was upregulated in ZhongmuNo.3 in this study, suggesting that the production of ATP and elimination of glycolytic products are effective strategies to acclimate the anaerobic conditions created by a saline environment in the tolerant genotype under salt stress. This result is inconsistent with the role of alcohol dehydrogenase in crop tolerance to flooding …”