Comparative Proteomics of Salt Tolerance in <i>Arabidopsis thaliana</i> and <i>Thellungiella halophila</i>

Pang, Qiuying; Chen, Sixue; Dai, Shaojun; Chen, Yazhou; Wang, Yang

doi:10.1021/pr100034f

Cited by 281 publications

(289 citation statements)

References 95 publications

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“…This technique is now widely applied to plants to understand changes in protein profiles during growth or due to environmental stimuli and stress (Lü cker et al, 2009;Schneider et al, 2009;Pang et al, 2010). However, few studies have directly evaluated the physiological importance of proteins identified by iTRAQ analysis.…”

Section: Discussionmentioning

confidence: 99%

iTRAQ Analysis Reveals Mechanisms of Growth Defects Due to Excess Zinc in Arabidopsis

et al. 2011

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(R.T., M.M.)The micronutrient zinc is essential for all living organisms, but it is toxic at high concentrations. Here, to understand the effects of excess zinc on plant cells, we performed an iTRAQ (for isobaric tags for relative and absolute quantification)-based quantitative proteomics approach to analyze microsomal proteins from Arabidopsis (Arabidopsis thaliana) roots. Our approach was sensitive enough to identify 521 proteins, including several membrane proteins. Among them, IRT1, an iron and zinc transporter, and FRO2, a ferric-chelate reductase, increased greatly in response to excess zinc. The expression of these two genes has been previously reported to increase under iron-deficient conditions. Indeed, the concentration of iron was significantly decreased in roots and shoots under excess zinc. Also, seven subunits of the vacuolar H + -ATPase (V-ATPase), a proton pump on the tonoplast and endosome, were identified, and three of them decreased significantly in response to excess zinc. In addition, excess zinc in the wild type decreased V-ATPase activity and length of roots and cells to levels comparable to those of the untreated de-etiolated3-1 mutant, which bears a mutation in V-ATPase subunit C. Interestingly, excess zinc led to the formation of branched and abnormally shaped root hairs, a phenotype that correlates with decreased levels of proteins of several root hair-defective mutants. Our results point out mechanisms of growth defects caused by excess zinc in which cross talk between iron and zinc homeostasis and V-ATPase activity might play a central role.

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Section: Discussionmentioning

confidence: 99%

iTRAQ Analysis Reveals Mechanisms of Growth Defects Due to Excess Zinc in Arabidopsis

et al. 2011

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“…Proteomics studies revealed that the expression of many components of protein synthesis machinery are altered under salt stress conditions and suggested that protein synthesis plays a vital role in abiotic stress adaptation Witzel et al 2009;Pang et al 2010). Our study demonstrated that serine carboxypeptidase (spot 181) and pollen allergen MetE (spot 717),respectively related to protein processing and amino acid metabolism were down-regulated under salt stress.…”

Section: Protein Processing and Amino Acid Metabolismmentioning

confidence: 60%

Integrated proteomics and metabolomics for dissecting the mechanism of global responses to salt and alkali stress in Suaeda corniculata

et al. 2016

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Background and aims Soil alkalinity and salinity are two of the most common environmental stress factors that impact plant growth and productivity. S. corniculata is native to the saline-alkali soil of Northeast China which shows a higher alkali tolerance than other suaeda plants. It will be very important to identify the effect of salt and alkali stress on S. corniculata through proteomics and metabolomics analysis to improve understanding of the resistance of plants. Methods S. corniculata seedlings were exposed to salt and alkali stress for 72 h, respectively. Metabolic changes were quantified by conducting MS-based proteomics and NMR-based metabolomics analysis.Results The response of S. corniculata to salt stress was distinct from that of plants subjected to alkali stress at both physiological and molecular levels. The integrated-omic studies identified 22 and 19 differentially expressed proteins and metabolites mainly involved in carbohydrate metabolism and amino acid metabolism. Conclusions On account of the up-regulation of energy metabolism and higher accumulation of organic osmolytes, S. corniculata shows high pH resistance when it suffers alkali stress. These findings provide insights into the different regulatory mechanisms in halophyte S. corniculata response to salt and alkali stresses.

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“…There, reduction in abundance of RubisCo large/small subunit, chloroplast precursor (OEE), Oxygen-evolving enhancer protein 2, carbonic anhydrases as well as Rubisco's activity leads to short assimilation. Fall in assimilation has been reported for many glycophyte plants subjected to salinity stress (Aghaei, 2008; Pang et al, 2010;Sobhanian et al, 2010;Chatlopadhyay 2011 andBandehagh et al, 2011). However, in some sensitive plants, including durum wheat, an increase in activity of RubisCo is thought to be a response rendered to support the carboxylase activities of RubisCo (Caruso et al, 2008).…”

Section: Protein Identificationmentioning

confidence: 99%

Canola Seedling Response to NaCl Stress – a Proteomic Approach

Alagoz

Toorchi

Bandehagh

2016

Not Bot Horti Agrobo

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Salinity is a major abiotic stress worldwide, which causes a tremendous loss in the quality and quantity of agricultural products. In order to identify defense mechanisms to salinity-induced stress, a two-dimensional electrophoresis approach was used to investigate proteins achieved from canola cultivar 'Option 500' seedlings, after plants had been treated with NaCl. The 300 mmol NaCl treatment caused a significant decrease in fresh and dry weight as well as plant height and increment of proline content compared with control group. There were no significant changes in the quality of catalase and peroxidase activity. Out of 110 repeatable protein spots, 44 were registering change, according to induction factor index. 7 spots were recognized significant at 1% probability level statistically. These proteins were involved in energy production, ROS scavenging and suppressor, and those involved in photosynthesis. Production of chloroplastic fructose-bisphosphate aldolase enzyme along with carbohydrate metabolisms of Kelvin cycle and glycolysis increases under salinity condition. The amount of Cu/Zn SOD in chloroplast reduces in response to salinity which results from an increase in OH -radicals. Expression of photosynthesis proteins have been reduced probably as a consequence of the drop in osmotic pressure resulted from water scarcity and stomata closure, which reduces the availability of Co2.

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Comparative Proteomics of Salt Tolerance in Arabidopsis thaliana and Thellungiella halophila

Cited by 281 publications

References 95 publications

iTRAQ Analysis Reveals Mechanisms of Growth Defects Due to Excess Zinc in Arabidopsis

iTRAQ Analysis Reveals Mechanisms of Growth Defects Due to Excess Zinc in Arabidopsis

Integrated proteomics and metabolomics for dissecting the mechanism of global responses to salt and alkali stress in Suaeda corniculata

Canola Seedling Response to NaCl Stress – a Proteomic Approach

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