Comparative proteomics reveals essential mechanisms for osmotolerance in Gluconacetobacter diazotrophicus

Leandro, Mariana Ramos; Andrade, Leandro Fernandes; Vespoli, Luciano de Souza; Moreira, Júlia Rosa; Pimentel, Vivian Ribeiro; Soares, Fabiano Silva; Passamani, Lucas Zanchetta; Silveira, Vanildo; Filho, Gonçalo A. de Souza

doi:10.1016/j.resmic.2020.09.005

Cited by 8 publications

(3 citation statements)

References 44 publications

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“…since a mannitol dehydrogenase gene was identified in all analyzed genomes ( Table S2 ). As shown for Gluconacetobacter diazotrophicus , osmoprotection in acetic acid bacteria is a multifactorial interplay of different cellular mechanisms [ 65 ]. Osmotic stress influences nutrient uptake, de novo saturated fatty acids biosynthesis, cell division and other factors, an impact too complex to predict by comparative genomic analysis alone, which should be the subject of future physiologic studies.…”

Section: Discussionmentioning

confidence: 99%

Comparative Genomics of Acetic Acid Bacteria within the Genus Bombella in Light of Beehive Habitat Adaptation

Härer¹,

Hilgarth²,

Ehrmann³

2022

Microorganisms

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It is known that the bacterial microbiota in beehives is essential for keeping bees healthy. Acetic acid bacteria of the genus Bombella colonize several niches in beehives and are associated with larvae protection against microbial pathogens. We have analyzed the genomes of 22 Bombella strains of different species isolated in eight different countries for taxonomic affiliation, central metabolism, prophages, bacteriocins and tetracycline resistance to further elucidate the symbiotic lifestyle and to identify typical traits of acetic acid bacteria. The genomes can be assigned to four different species. Three genomes show ANIb values and DDH values below species demarcation values to any validly described species, which identifies them as two potentially new species. All Bombella spp. lack genes in the Embden–Meyerhof–Parnas pathway and the tricarboxylic acid cycle, indicating a focus of intracellular carbohydrate metabolism on the pentose phosphate pathway or the Entner–Doudoroff pathway for which all genes were identified within the genomes. Five membrane-bound dehydrogenases were identified that catalyze oxidative fermentation reactions in the periplasm, yielding oxidative energy. Several complete prophages, but no bacteriocins, were identified. Resistance to tetracycline, used to prevent bacterial infections in beehives, was only found in Bombella apis MRM1T. Bombella strains exhibit increased osmotolerance in high glucose concentrations compared to Gluconobacter oxydans, indicating adaption to high sugar environments such as beehives.

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Section: Discussionmentioning

confidence: 99%

Comparative Genomics of Acetic Acid Bacteria within the Genus Bombella in Light of Beehive Habitat Adaptation

Härer¹,

Hilgarth²,

Ehrmann³

2022

Microorganisms

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“…Results obtained in this study showed that Gluconacetobacter diazotrophicus Pal5 strain may better colonize maize plants under moderate drought, low nitrogen, and combined stress based on the nifH and Gd 16S rRNA genes analysis. This might be due to the tolerance mechanism of G. diazotrophicus decreasing the level of drought stress [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

Gluconacetobacter diazotrophicus Pal5 Enhances Plant Robustness Status under the Combination of Moderate Drought and Low Nitrogen Stress in Zea mays L.

et al. 2021

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Plant growth promoting endophytic bacteria, which can fix nitrogen, plays a vital role in plant growth promotion. Previous authors have evaluated the effect of Gluconacetobacter diazotrophicus Pal5 inoculation on plants subjected to different sources of abiotic stress on an individual basis. The present study aimed to appraise the effect of G. diazotrophicus inoculation on the amelioration of the individual and combined effects of drought and nitrogen stress in maize plants (Zea mays L.). A pot experiment was conducted whereby treatments consisted of maize plants cultivated under drought stress, in soil with a low nitrogen concentration and these two stress sources combined, with and without G. diazotrophicus seed inoculation. The inoculated plants showed increased plant biomass, chlorophyll content, plant nitrogen uptake, and water use efficiency. A general increase in copy numbers of G. diazotrophicus, based on 16S rRNA gene quantification, was detected under combined moderate stress, in addition to an increase in the abundance of genes involved in N fixation (nifH). Endophytic colonization of bacteria was negatively affected by severe stress treatments. Overall, G. diazotrophicus Pal5 can be considered as an effective tool to increase maize crop production under drought conditions with low application of nitrogen fertilizer.

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“…In addition, a subunit of the rst enzyme in the biosynthesis pathway of fatty acids (acetyl-CoA-carboxylase), was also identi ed as up-regulated in the presence of root exudates (Figure 3). Gluconacetobacter diazotrhophicus Pal5 mutants in another subunit of this enzyme are affected at high osmotic environments, highlighting its role in the adaptation to osmotic stress (Leandro et al 2021). Moreover, fatty acids and modi ed fatty acids were described as important molecules during plant colonization by microorganisms (Pohl and Kock 2014; Uranga et al 2016).…”

Section: Nutrient Transport and Metabolismmentioning

confidence: 99%

Modulation of the endophytic strain Kosakonia radicincitans UYSO10 proteome by sugarcane roots exudate

Taulé,

Lima,

Beracochea

et al. 2024

Preprint

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Backgroun: Plant-associated microbiotas play a key role in plant health, growth, and stresses resilience. These microbiotas are considered the second plant genome by expanding its genetic potential. One of the main components of the plant microbiota is the endophytic bacterial communities, which live in the plant's internal tissues. The main sources of this microbiota are the seed and the soil where the plant grows. Particularly, soil bacteria are attracted by different signals present in plant root exudates, to later colonize the rhizoplane and infect internal tissues. Although the colonization and infection processes of endophytic bacteria are well documented, the molecular bases of the mechanisms involved in the plant-endophyte interaction are still poorly understood. Previously it was shown that strain Kosakonia radicincitans UYSO10 promotes the growth of sugarcane plants and was defined as a true endophyte. Moreover, it was demonstrated that the biological nitrogen fixation process is involved in the plant growth promotion observed. The aim of this work is to expand the knowledge about the possible mechanisms involved in the early stages of the interaction between the diazotrophic endophytic strain UYSO10 and sugarcane plants. Methodology: a proteomic approach was conducted in the strain UYSO10 exposed or not to sugarcane exudates. Results showed that in the presence of root exudates, strain UYSO10 senses the environment and adapts its proteome to transport and metabolize different nutrients, and to interact with the host plant. These results deepen the knowledge of the potential mechanisms involved in the early stage of plant-bacteria endophyte interaction.

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Comparative proteomics reveals essential mechanisms for osmotolerance in Gluconacetobacter diazotrophicus

Cited by 8 publications

References 44 publications

Comparative Genomics of Acetic Acid Bacteria within the Genus Bombella in Light of Beehive Habitat Adaptation

Comparative Genomics of Acetic Acid Bacteria within the Genus Bombella in Light of Beehive Habitat Adaptation

Gluconacetobacter diazotrophicus Pal5 Enhances Plant Robustness Status under the Combination of Moderate Drought and Low Nitrogen Stress in Zea mays L.

Modulation of the endophytic strain Kosakonia radicincitans UYSO10 proteome by sugarcane roots exudate

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