Comparative quantitative analysis of ultraviolet B‐induced skin blood flow change using laser Doppler perfusion imaging technique

Abstract

Laser Doppler perfusion imaging (LDI), recently developed, can generate a color-coded image of tissue perfusion, making it possible to assess the spatial distribution of skin perfusion without touching the surface. Using this apparatus, we investigated ultraviolet B (UVB)-induced perfusion profile of the skin and compared the results with those obtained from laser Doppler flowmetry (LDF). Fifteen adult male Korean volunteers were irradiated with several doses of UVB ranging from 25 mJ/cm2 to 186 mJ/cm2. Twenty-four hours later, the erythema reaction was evaluated with LDF and LDI systems. There was a significant correlation between the logarithmic dose of UVB and erythema values. The curves consisted of two parts, an initial, flat phase and then a linear, steep one. Also, there was a good correlation between LDF and LDI. The LDI is as sensitive as conventional LDF, but has the many advantages of measuring blood flow over large areas without contact with the skin surface. This instrument will be useful in the measurement of skin blood flow in many areas of dermatological application.

Phototesting with a divergent UVB beam in the investigation of anti‐inflammatory effects of topically applied substances

“…The control and test reactions clearly illustrate the linear relationship between dose and skin perfusion in the dose range used here (up to approximately 1.5 MED). This result can be compared with the reports by other investigators (15,16), claiming the existence of a linear relationship between the logarithm of the irradiation dose and the resultant skin response. This discrepancy can be explained by the greater dose interval used in these studies, three times the MED in the study of Youn and et al (16) and 15 times the MED in that of Farr and co-workers (15).…”

“…This result can be compared with the reports by other investigators (15,16), claiming the existence of a linear relationship between the logarithm of the irradiation dose and the resultant skin response. This discrepancy can be explained by the greater dose interval used in these studies, three times the MED in the study of Youn and et al (16) and 15 times the MED in that of Farr and co-workers (15). Other explanations may be the traditional provocation method employed in these studies, using homogeneous irradiance fields applied to separate, small skin areas for each dose tested.…”

Laser Dopplers to Determine Cutaneous Blood Flow

“…Various models of inflammatory reactions have been studied by both conventional laser Doppler probes and LDIs. Vascular responses to the tuberculin test, 29 patch tests for allergic contact hypersensitivity, 43 and a range of ultraviolet B doses 44 were measured to observe changes in the area and intensity of these inflammatory reactions. These studies also provided opportunities to compare the two laser Doppler instruments, and overall, relative changes in their flux measurements were highly correlated 29,43 …”

Topical application of superoxide dismutase mediated by HIV-TAT peptide attenuates UVB-induced damages in human skin

“…It was measured as red blood cell flux using a laser Doppler perfusion monitor. In previous studies, both Andersen and Youn suggested that the blood flow response after UVB irradiation are divided into two phases depending on doses of UVB irradiation [31,32]. The cut-off point between two phases is around MED.…”