Comparative Secretome Analyses of Human and Zoonotic Staphylococcus aureus Isolates CC8, CC22, and CC398

Busche, Tobias; Hillion, Mélanie; Loi, Vu Van; Berg, David T.; Walther, Birgit; Semmler, Torsten; Strommenger, Birgit; Witte, Wolfgang; Cuny, Christiane; Mellmann, Alexander; Holmes, Mark A.; Kalinowski, Jörn; Adrian, Lorenz; Bernhardt, Jörg; Antelmann, Haike

doi:10.1074/mcp.ra118.001036

Cited by 32 publications

(33 citation statements)

References 114 publications

(201 reference statements)

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“…It could be related to the mode of transmission, with close contact with animals. In contrast, fewer cases of MSSA CC398 RTI have been reported [7,8,16,19,20,27,29,37,39,50,55,61,62,68,74]. Hence, a French study of 89 clinical isolates of MSSA CC398 reported that 12% were isolated from pneumonia, 22% from BSI and 29% from SSTI [19].…”

Section: Respiratory Tract Infectionsmentioning

confidence: 99%

Human Infection of Methicillin-Susceptible Staphylococcus aureus CC398: A Review

et al. 2020

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Staphylococcus aureus (SA) belonging to the clonal complex 398 (CC398) took a special place within the species due to its spread throughout the world. SA CC398 is broadly separated in two subpopulations: livestock-associated methicillin-resistant SA (MRSA) and human-associated methicillin-susceptible SA (MSSA). Here, we reviewed the global epidemiology of SA CC398 in human clinical infections and focused on MSSA CC398. The last common ancestor of SA CC398 was probably a human-adapted prophage φSa3-positive MSSA CC398 strain, but the multiple transmissions between human and animal made its evolution complex. MSSA and MRSA CC398 had different geographical evolutions. Although MSSA was present in several countries all over the world, it was mainly reported in China and in France with a prevalence about 20%. MSSA CC398 was frequently implicated in severe infections such as bloodstream infections, endocarditis, and bone joint infections whereas MRSA CC398 was mainly reported in skin and soft tissue. The spread of the MSSA CC398 clone is worldwide but with a heterogeneous prevalence. The prophage φSa3 played a crucial role in the adaptation to the human niche and in the virulence of MSSA CC398. However, the biological features that allowed the recent spread of this lineage are still far from being fully understood.

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Section: Respiratory Tract Infectionsmentioning

confidence: 99%

Human Infection of Methicillin-Susceptible Staphylococcus aureus CC398: A Review

et al. 2020

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“…Many studies have used liquid culture medium to identify the secretome from pathogenic microbes, eg. Xanthomonas oryzae, Botrytis cinerea , and Staphylococcus aureus [34-37]. In the present study, we used a quantitative proteomics approach to analyze the secretome of M. oryzae and identified 558 proteins from the liquid culture medium, including 411 proteins of the wild-type P131 strain and 539 proteins of the Δ alg3 mutant strain.…”

Section: Discussionmentioning

confidence: 99%

Comparative secretome ofMagnaporthe oryzaeidentified proteins involved in virulence and cell wall integrity

Liu

Huang

et al. 2020

Preprint

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Plant fungal pathogens secrete numerous proteins into the apoplast at the plant–fungus contact sites to facilitate colonization. Only a few secreted proteins were functionally characterized in Magnaporthe oryzae, the fungal pathogen causing rice blast disease worldwide. ALG3 is an α-1, 3-mannosyltransferase function in N-glycan synthesis for secreted N-glycosylated proteins, and the Δalg3 mutants show strong defects in cell wall integrity and fungal virulence, indicating a potential effect on the secretion of multiple proteins. In this study, we compared the secretome of wild type and Δalg3 mutants, and identified 51 proteins that require ALG3 for proper secretion. These are predicted to be involved in metabolic processes, interspecies interactions, cell wall organization, and response to chemicals. The tested secreted proteins localized at the apoplast region surrounding the fungal infection hyphae. Moreover, the N-glycosylation of candidate proteins was significantly changed in the Δalg3 mutant, leading to the reduction of protein secretion and abnormal protein localization. Furthermore, we tested the function of two genes, one is a previously reported M. oryzae gene Invertase 1 (INV1) encoding a secreted invertase, and the other one is a gene encoding an Acid mammalian chinitase (AMCase). The fungal virulence was significantly reduced and the cell wall integrity was altered in the Δinv1 and Δamcase mutant strains. Elucidation of the comparative secretome of M. oryzae improves our understanding of the proteins that require ALG3 for secretion, and of their function in fungal virulence and cell wall integrity.

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“…Hla is secreted by approximately 95% of clinical S. aureus strains, with observable trends in distinct clonal types, such as an increase in Hla expression in CC398 strains, which correlates with stronger hemolysin activity [ 6 ]. Interestingly, CC398 is a commonly identified lineage of livestock-associated MRSA that can infect pigs as well as humans.…”

Section: Role Of S Aureus Toxins In Human Disementioning

confidence: 99%

“…This study found that LA-MRSA isolates are highly cytotoxic in comparison with human HA- and CA-MRSA isolates but are less adherent to human cells than human isolates. All tested LA-MRSA CC398 strains also expressed hla when tested by qRT-PCR [ 6 , 7 ]. Hla has been notably associated with colonization of individuals who go on to develop invasive disease.…”

Section: Role Of S Aureus Toxins In Human Disementioning

confidence: 99%

Epidemiological and Clinical Evidence for the Role of Toxins in S. aureus Human Disease

Bennett

Thomsen

2020

Toxins

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Staphylococcus aureus asymptomatically colonizes approximately 30–50% of the population and is a leading cause of bacteremia, bone/joint infections, and skin infections in the US. S. aureus has become a major public health threat due to antibiotic resistance and an increasing number of failed vaccine attempts. To develop new anti-staphylococcal preventive therapies, it will take a more thorough understanding of the current role S. aureus virulence factors play in contributing to human disease. This review focuses on the clinical association of individual toxins with S. aureus infection as well as attempted treatment options. Further understanding of these associations will increase understanding of toxins and their importance to S. aureus pathogenesis.

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Comparative Secretome Analyses of Human and Zoonotic Staphylococcus aureus Isolates CC8, CC22, and CC398

Cited by 32 publications

References 114 publications

Human Infection of Methicillin-Susceptible Staphylococcus aureus CC398: A Review

Human Infection of Methicillin-Susceptible Staphylococcus aureus CC398: A Review

Comparative secretome ofMagnaporthe oryzaeidentified proteins involved in virulence and cell wall integrity

Epidemiological and Clinical Evidence for the Role of Toxins in S. aureus Human Disease

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