Comparative sensitivity of the echovirus type 25 JV-4 prototype strain and two recent isolates to glutaraldehyde at low concentrations

Abstract

The sensitivity of two recently isolated antigenic variants of echovirus type 25 (Montpellier 76-1262 and Thionville 86-222) to glutaraldehyde (GTA) at low concentrations was compared with that of the JV-4 prototype strain. The purified viruses were treated under the same conditions with GTA at concentrations ranging from 0.002 to 0.10%X. The wild strains exhibited significantly lower sensitivity to GTA than did the prototype strain; with 0.10%/c GTA, a 2 log10 unit reduction was obtained in 5 min for JV-4 and in 60 and 80 min for Montpellier 76-1262 and Thionville 86-222, respectively. A comparison with previous results obtained with poliovirus type 1 showed that the inactivation rates of echovirus type 25 wild strains were fivefold lower than those of the poliovirus type 1 Sabin strain. The comparative electrophoretic and immunoblot analyses showed differences in the results of GTA binding with capsid proteins of the viruses. Unlike in the poliovirus type 1 Mahoney strain and in the echovirus type 25 JV-4 reference strain, GTA produced only minor intermolecular cross-linkings in the viral particles of the two wild strains of echovirus type 25. Our results suggest that there are both intertypic and intratypic differences in the GTA sensitivities of enterovirus strains. They are of relevance to disinfection procedures in digestive endoscopy and to the choice of the enterovirus strain used for evaluating the efficacy of disinfectants.

Comparative sensitivities of Sabin and Mahoney poliovirus type 1 prototype strains and two recent isolates to low concentrations of glutaraldehyde

“…At the highest GTA concentration tested (0.10%), a departure from linearity was observed for these two strains. We have already reported such departures from linearity in inactivation process with echovirus type 25 strains (7). We observed that the inactivation kinetics of the JV-4 reference strain differed strongly from those of the two recently isolated variants tested, which were more resistant.…”

“…In a previous study, we reported that two recently isolated variants of echovirus type 25 exhibited significantly lower sensitivity to GTA than did their corresponding prototype strain JV-4 (7). In this study, we determined whether such differences in the sensitivity to GTA can be observed between PV-1 isolates currently circulating in the population and the reference strains.…”

“…This test was chosen because it uses a genetic marker in the VP1 capsid protein-coding region that characterizes the strains. Furthermore, the VP1 polypeptide was predicted to be involved in GTA binding (6,7).…”

“…The aim of this study was to determine whether these antigenic differences are related to the different sensitivities of poliovirus type 1 (PV-1) strains to glutaraldehyde (GTA). In a previous study (7), we reported that two recent clinical isolates of echovirus type 25 differed from the reference strain of echovirus type 25 in their sensitivity to low concentrations of GTA. In the present study, we compared the level of sensitivity to GTA, under the same conditions, of the two PV-1 prototype strains (vaccine and wild strain) with those of two recent natural isolates of PV-1.…”

The influence of stabilizers and rates of freezing on preserving of structurally different animal viruses during lyophilization and subsequent storage

“…The responses of viruses of the same family to additives and freezing rates during lyophilization and storage were similar in the present study. Nevertheless, other studies have shown that even closely related viruses can differ in their sensitivity to identical stress, due to certain molecular differences in the proteins that make up viral particles (Coleman et al 1973;Chambon et al 1994;Bozkurt et al 2013). The best stabilizing activity ascertained here was for gelatine in combination with sucrose as an additive for all enveloped viruses; the same stabilizer also performed no worse than the others in the nonenveloped adenoviruses and caliciviruses.…”