Comparative Sensitivity of Trichophyton and Aspergillus Conidia to Inactivation by Violet-Blue Light Exposure

Moorhead, Sian; Maclean, Michelle; MacGregor, S.J.; Anderson, John G.

doi:10.1089/pho.2015.3922

Cited by 22 publications

(9 citation statements)

References 26 publications

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“…The final 79 research articles reviewed contained inactivation data on clinically relevant and food‐associated microorganisms exposed to violet‐blue light between 380 and 480 nm. Data from each article were extracted, summarized and tabulated (see Supporting Information).…”

Section: Methodsmentioning

confidence: 99%

“…For example, Haughton et al (2012) inactivated Campylobacter jejuni using 395 nm light, while Bumah et al (14,15) demonstrated the antimicrobial efficacy of 470 nm light against Salmonella enterica and S. aureus. Additionally, a small number of bacterial endospores, fungi and yeasts have been inactivated using violet-blue light (16)(17)(18)(19)(20)(21)(22)(23). To date, little is known about viral susceptibility; however, there is now published evidence demonstrating 405 nm light inactivation of a viral surrogate, bacteriophage ɸC31, and a mammalian virus, feline calicivirus, without the requirement of additional photosensitizers (24,25).…”

Section: Introductionmentioning

confidence: 99%

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Review of the Comparative Susceptibility of Microbial Species to Photoinactivation Using 380–480 nm Violet‐Blue Light

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Coia

et al. 2018

Photochem & Photobiology

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Antimicrobial violet-blue light is an emerging technology designed for enhanced clinical decontamination and treatment applications, due to its safety, efficacy and ease of use. This systematized review was designed to compile the current knowledge on the antimicrobial efficacy of 380-480 nm light on a range of health care and food-related pathogens including vegetative bacteria, bacterial endospores, fungi and viruses. Data were compiled from 79 studies, with the majority focussing on wavelengths in the region of 405 nm. Analysis indicated that Gram-positive and Gram-negative vegetative bacteria are the most susceptible organisms, while bacterial endospores, viruses and bacteriophage are the least. Evaluation of the dose required for a 1 log 10 reduction of key bacteria compared to population, irradiance and wavelength indicated that microbial titer and light intensity had little effect on the dose of 405 nm light required; however, linear analysis indicated organisms exposed to longer wavelengths of violet-blue light may require greater doses for inactivation. Additional research is required to ensure this technology can be used effectively, including: investigating inactivation of multidrug-resistant organisms, fungi, viruses and protozoa; further knowledge about the photodynamic inactivation mechanism of action; the potential for microbial resistance; and the establishment of a standardized exposure methodology.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Review of the Comparative Susceptibility of Microbial Species to Photoinactivation Using 380–480 nm Violet‐Blue Light

Tomb

White

Coia

et al. 2018

Photochem & Photobiology

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“…Moorhead et al used 405-nm aBL for inhibiting the growth of Trichophyton rubrum , Trichophyton mentagrophytes , and Aspergillus niger (Moorhead et al, 2016b). On agar plates, the growth of the microconidia of T. rubrum and T. mentagrophytes was completely inhibited after an exposure of 504 J/cm 2 aBL.…”

Section: Efficacy Of Antimicrobial Blue Light Inactivation Of Pathmentioning

confidence: 99%

Antimicrobial blue light inactivation of pathogenic microbes: State of the art

Wang

et al. 2017

Drug Resistance Updates

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As an innovative non-antibiotic approach, antimicrobial blue light in the spectrum of 400–470 nm has demonstrated its intrinsic antimicrobial properties resulting from the presence of endogenous photosensitizing chromophores in pathogenic microbes and, subsequently, its promise as a counteracter of antibiotic resistance. Since we published our last review of antimicrobial blue light in 2012, there have been a substantial number of new studies reported in this area. Here we provide an updated overview of the findings from the new studies over the past 5 years, including the efficacy of antimicrobial blue light inactivation of different microbes, its mechanism of action, synergism of antimicrobial blue light with other angents, its effect on host cells and tissues, the potential development of resistance to antimicrobial blue light by microbes, and a novel interstitial delivery approach of antimicrobial blue light. The potential new applications of antimicrobial blue light are also discussed.

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“…Up to now the elimination of fungi by light treatment has been studied with single-photon excitation (see Wang et al, 2017 for a review). These studies made it possible to demonstrate the possibility of inhibiting growth or even inactivating certain fungi by light treatment without using exogenous photosensitizers (Murdoch et al, 2013 ; Moorhead et al, 2016 ; Guffey et al, 2017 ; Trzaska et al, 2017 ) and to identify certain endogenous photosensitizers such as porphyrins (Fraikin et al, 1996 ). However, little is known of the initiation site of oxidation, the intra-cellular dynamics of dispersion and the effect of power excitation modulation with two-photon excitation.…”

Section: Discussionmentioning

confidence: 99%

Cell Death Mechanisms Induced by Photo-Oxidation Studied at the Cell Scale in the Yeast Saccharomyces cerevisiae

et al. 2018

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Blue light (400–430 nm) is known to induce lethal effects in some species of fungi by photo-oxidation caused by the excitation of porphyrins but the mechanisms involved remain poorly understood. In this work, we exposed the yeast Saccharomyces cerevisiae to a high density light flux with two-photon excitation (830 nm equivalent to a one-photon excitation around 415 nm) and used quasi real-time visualization with confocal microscopy to study the initiation and dynamics of photo-oxidation in subcellular structures. Our results show that the oxidation generated by light treatments led to the permeabilization of the plasma membrane accompanied by the sudden expulsion of the cellular content, corresponding to cell death by necrosis. Moreover, excitation in the plasma membrane led to very fast oxidation and membrane permeabilization (<60 s) while excitation at the center of the cell did not induce permeabilization even after a period exceeding 600 s. Finally, our study shows that the relationship between the laser power used for two-photon excitation and the time required to permeabilize the plasma membrane was not linear. Thus, the higher the power used, the lower the energy required to permeabilize the plasma membrane. We conclude that fungal destruction can be generated very quickly using a high density light flux. Better knowledge of the intracellular processes and the conditions necessary to induce necrosis should make it possible in the future to improve the efficiency of antimicrobial strategies photo-oxidation-based.

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Comparative Sensitivity of Trichophyton and Aspergillus Conidia to Inactivation by Violet-Blue Light Exposure

Cited by 22 publications

References 26 publications

Review of the Comparative Susceptibility of Microbial Species to Photoinactivation Using 380–480 nm Violet‐Blue Light

Review of the Comparative Susceptibility of Microbial Species to Photoinactivation Using 380–480 nm Violet‐Blue Light

Antimicrobial blue light inactivation of pathogenic microbes: State of the art

Cell Death Mechanisms Induced by Photo-Oxidation Studied at the Cell Scale in the Yeast Saccharomyces cerevisiae

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