Comparative studies by comet test and SCE analysis in human lymphocytes from 200 healthy subjects

Betti, Cecilia; Davini, Tania; Giannessi, L.; Loprieno, N.; Barale, Roberto

doi:10.1016/0165-1218(95)90015-2

Cited by 130 publications

(43 citation statements)

References 10 publications

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“…The comet assay has been previously found applicable to screening large numbers of individuals to determine the extent of DNA damage in human lymphocytes (Betti et al, 1995). We propose that the assay is also suitable for screening DNA damage after PDT and thermal laser treatments and offers some advantages over existing methods of measuring DNA damage.…”

Section: Discussionmentioning

confidence: 99%

A comet assay of DNA damage and repair in K562 cells after photodynamic therapy using haematoporphyrin derivative, methylene blue and meso-tetrahydroxyphenylchlorin

McNair

Marples²,

West³

et al. 1997

Br J Cancer

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Summary Single-cell electrophoresis (comet assay) has been used to evaluate DNA damage and repair in the human myeloid leukaemia cell line K562 after low-dose (predominantly sub-lethal) treatments of hyperthermia and photodynamic therapy (PDT). Three different photosensitizers were examined: haematoporphyrin derivative (HpD), methylene blue (MB) and meso-tetrahydroxyphenylchlorin (mTHPC). None of the drugs in the absence of light, nor in light alone, resulted in detectable DNA damage. However, a significant amount of DNA damage was detected immediately after treatment with haematoporphyrin derivative or methylene blue PDT compared with drug-only or lightonly treatments; no residual level of DNA damage was evident for either drug following a 4-h post-treatment incubation at 370C. No significant DNA damage was detected after meso-tetrahydroxyphenylchlorin PDT or hyperthermia either immediately or 4 h after treatment. We conclude that the alkaline comet assay can be applied as an effective screening assay for DNA damage induced by a range of laser therapies.

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Section: Discussionmentioning

confidence: 99%

A comet assay of DNA damage and repair in K562 cells after photodynamic therapy using haematoporphyrin derivative, methylene blue and meso-tetrahydroxyphenylchlorin

McNair

Marples²,

West³

et al. 1997

Br J Cancer

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“…The assay is increasingly used in biomonitoring and epidemiological studies [Betti et al, 1994[Betti et al, , 1995Collins et al, 1997;Kassie et al, 2000;Dhawan et al, 2001;Bajpayee et al, 2002;Faust et al, 2004], where it is most often used to detect DNA damage in peripheral lymphocytes. Using the Comet assay, higher levels of DNA damage have been reported among persons exposed to a variety of chemicals [Hartmann et al, 1995;Yuspa and Shields, 1997;Awara et al, 1998;Andreoli et al, 1999;Lei et al, 2004] and ionizing radiation [Singh et al, 1995;Nascimento et al, 2001].…”

Section: Introductionmentioning

confidence: 99%

DNA damage in lymphocytes of rural Indian women exposed to biomass fuel smoke as assessed by the Comet assay

Pandey

Bajpayee

Parmar

et al. 2005

Environ. Mol. Mutagen.

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The Comet assay has found wide acceptance in monitoring human genotoxicity caused by lifestyle and occupational and environmental factors. In the present study, we have used the Comet assay to measure the DNA damage in a population of rural Indian women cooking with biomass fuels (BMFs; fire wood and cow dung cakes). Out of 144 volunteers, 70 used BMFs for domestic cooking, while the remaining 74 used liquefied petroleum gas (LPG) and served as a reference population. All the individuals had comparable socioeconomic backgrounds and were between 20 and 55 years of age. Significantly higher levels of DNA damage were observed for BMF users than for LPG users. For BMF users in comparison with the reference population, Olive tail moment was 3.83 +/- 0.15 (arbitrary units) vs. 2.77 +/- 0.07 (P < 0.001); % tail DNA was 11.19 +/- 0.35 vs. 8.29 +/- 0.20 (P < 0.001); and comet tail length (microm) was 51.15 +/- 1.37 vs. 40.26 +/- 0.88 (P < 0.001). Similar significant differences were found when the groups were stratified by age and length of exposure. This study suggests that exposure to BMF smoke leads to greater levels of DNA damage than exposure to LPG combustion products.

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“…In research today, the majority of studies on the genotoxicity of smoking using the Comet assay have reported that smoking induces DNA damage in human populations [10,11,13,16,23]. On the other hand, reports of failure to detect such an effect have been widely published in recent years [12,14,15,17].…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, the ability to assess DNA damage in human cells using valid methods may help reduce the carcinogenic risks during early phases of carcinogenesis [5][6][7]. Over the last decade, interest has increased in single-cell gel electrophoresis or Comet assay principally due to their simplicity and sensitivity in comparison to other conven-tional cytogenetic techniques of monitoring DNA damage, including chromosomal aberration, sister chromatid exchange, or micronuclei [8][9][10]. The Comet assay has been extensively used in human biomonitoring studies to detect DNA damage caused by lifestyle [10,11], food habits [12,13], and occupational [14,15] and environmental exposures [16,17], and it is also used as an effective biomarker in intervention studies of cancer [18,19] and other degenerative diseases [20,21].…”

Section: Introductionmentioning

confidence: 99%

Smoking and High Plasma Triglyceride Levels as Risk Factors for Oxidative DNA Damage in the Korean Population

Park

Kang²

2004

Ann Nutr Metab

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Aims: To investigate the effects of factors including dietary habits, lifestyle, anthropometric characteristics, plasma lipid profiles, and antioxidants on DNA damage among the Korean population. Methods: Blood samples were collected from 109 healthy Korean volunteers, aged 19–28 years, of whom 34% were smokers. Epidemiological information was collected by personal interviews, and anthropometric characteristics were measured directly. Oxidative DNA damage was quantified using the Comet assay; tail length (TL) and tail moment (TM) were measured. Results: Statistically significant (p < 0.05) positive correlations were observed between DNA damage (TM or TL) and smoking habits expressed as cigarettes smoked per day and number of packs smoked over time (r = 0.332 and 0.370, respectively, for TM; r = 0.266 and 0.304 for TL, respectively). There were also significant and positive correlations between DNA damage parameters and waist-hip ratio (r = 0.352 for TM and r = 0.226 for TL), but this significance disappeared after data were adjusted to account for smoking effects. Higher plasma triglyceride levels were associated with increased damage to DNA (r = 0.234 for TM and r = 0.271 for TL). Conclusions: Our results indicate that cigarette smoking and high plasma triglyceride levels significantly increase DNA damage to peripheral lymphocytes in a sample of Koreans.

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Comparative studies by comet test and SCE analysis in human lymphocytes from 200 healthy subjects

Cited by 130 publications

References 10 publications

A comet assay of DNA damage and repair in K562 cells after photodynamic therapy using haematoporphyrin derivative, methylene blue and meso-tetrahydroxyphenylchlorin

A comet assay of DNA damage and repair in K562 cells after photodynamic therapy using haematoporphyrin derivative, methylene blue and meso-tetrahydroxyphenylchlorin

DNA damage in lymphocytes of rural Indian women exposed to biomass fuel smoke as assessed by the Comet assay

Smoking and High Plasma Triglyceride Levels as Risk Factors for Oxidative DNA Damage in the Korean Population

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