DNA damage in lymphocytes of rural Indian women exposed to biomass fuel smoke as assessed by the Comet assay

Pandey, Alok Kumar; Bajpayee, Mahima; Parmar, Devendra; Rastogi, Shivani; Mathur, Neeraj; Seth, Prahlad K.; Dhawan, Alok

doi:10.1002/em.20106

Cited by 44 publications

(23 citation statements)

References 43 publications

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“…There were also mild chronic inflammation and squamous metaplasia in the larynx, alveolar macrophage hyperplasia, and slight thickening of the alveolar septa [16]. Wood smoke can cause greater levels of DNA damage in lymphocytes than exposure to liquefied petroleum gas combustion products [17], and can both impair macrophage function and be mutagenic [11].…”

Section: Characteristics and Toxicity Of Solid Fuel Usementioning

confidence: 99%

Indoor air pollution and the lung in low- and medium-income countries

2012

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Over half the world's population, mostly from developing countries, use solid fuel for domestic purposes and are exposed to very high concentrations of harmful air pollutants with potential health effects such as respiratory problems, cardiovascular problems, infant mortality and ocular problems. The evidence also suggests that, although the total percentage of people using solid fuel is decreasing, the absolute number is currently increasing. Exposure to smoke from solid fuel burning increases the risk of chronic obstructive pulmonary disease (COPD) and lung cancer in adults, and acute lower respiratory tract infection/pneumonia in children. Despite the heterogeneity among studies, the association between COPD and exposure to smoke produced by burning different types of solid fuel is consistent. However, there is strong evidence that while coal burning is a risk factor for lung cancer, exposure to other biomass fuel smoke is less so. There is some evidence that reduction of smoke exposure using improved cooking stoves reduces the risk of COPD and, possibly, acute lower respiratory infection in children, so approaches to reduce biomass smoke exposure are likely to result in reductions in the global burden of respiratory disease.

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Section: Characteristics and Toxicity Of Solid Fuel Usementioning

confidence: 99%

Indoor air pollution and the lung in low- and medium-income countries

2012

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“…The comet assay has gained wide acceptance in monitoring human genotoxicity caused by lifestyle and occupational and environmental factors (24). Comet assay is based on the assumption that DNA migrating from the nucleus within the gel after electrophoresis is the result of genotoxic damage that is converted to DNA single-or double-strand breaks.…”

Section: Discussionmentioning

confidence: 99%

Effect of cigarette smoking on DNA damage according to nine comet assay parameters in female and male groups

KAYAALTI¹

2012

Ankara Üniversitesi Tıp Fakültesi Mecmuası

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Aim:Smoking poses a serious threat to public health. The aim of this study was to investigate the relationship between smoking and DNA damage in lymphocytes. A potential genotoxic effect of cigarette smoking was analyzed with the nine comet assay parameters including comet length (CL), comet intensity (CI), head length (HL), head intensity (HI), tail length (TL), tail intensity (TI), DNA tail (DNAt), tail moment (TM) and olive tail moment (OTM). For the first time in this study, smokers were grouped as female and male, and nine comet parameters were used.Material and Method: 120 volunteers (60 non-smokers, 60 smokers) were monitored in the way of DNA damage in blood lymphocytes. The levels of DNA damage was measured by BAB Bs Comet Assay system.Results: Highly significant associations were found between the non-smoker and smoker groups for CI, TL and OTM comet parameters (p<0.01). Smoker female group had higher CL, CI, HL, HI, TL, TI (p<0.01) and TM (p<0.05) with regard to DNA damages than the non-smoker female group. In contrast, only DNAt, and OTM comet parameters were statistically significantdifferences between the smoker male and non-smoker male groups (p<0.05). When the smoking index (SI) of all the blood samples from females were compared based on all studied comet parameters, statistically significant association was found except for TM. On the other hand, the blood samples taken from males were statistically significant in terms of CL, HL, HI, TI and OTM parameters (p<0.05). Conclusion:Consequently, it can be said that, smoking cause DNA damages and females are more sensitive to the effect of the smoking than males.

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“…Biomass smoke inhalation causes oxidative stress and DNA damage in lungs and it is said to be the possible mechanism for pathogenesis of COPD 19 . Indian study by Pandey et al also demonstrated DNA damage in lymphocytes of women exposed to biomass smoke and impaired macrophage function 20 Particulate matter PM10 and PM 2.5 can be inhaled deeply into the lungs. The pathology due to biomass smoke exposure leads to respiratory tract infections, wheezing, chronic bronchitis and chronic obstructive pulmonary diseases.…”

Section: Health Effects Of Biomass Smoke Exposurementioning

confidence: 97%

Household Air pollution due to Biomass smoke exposure and Chronic Obstructive Pulmonary Disease

Fernandes¹,

Mesquita²

2014

Int J of Biomed & Adv Res

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Biomass fuel consists of wood, dried animal dung, crop residue, leaves, twigs and charcoal (product of incomplete burning of wood) which is cheap and easily available. It is estimated that one half of the world's population, approximately 3 billion people worldwide use biomass fuel for cooking and heating purpose, 2.4 billion burn biomass and 0.4 billion use coal 1 . These 3 billion are at risk of adverse health effects due to exposure to biomass smoke. 90% of rural population of the world and 75% of Indian population (700 million people) use biomass fuel for cooking and heating purposes 2 . 75% of rural homes and 22% urban homes in India use biomass fuel 3 . India (27%) and China (25%) together account for half the global population that uses biomass fuels for cooking. The use of biomass fuel depends on socio-economic status, availability of biomass, unavailability of cleaner fuels like electricity or LPG, traditions, non availability of cooking space to burn biomass fuel in urban households and awareness. Highest exposures to indoor air pollution occur during burning of biomass fuel during the cooking process. Women who are the principal cooks of the family, young children being cared by their mothers (carried by mothers on their backs during cooking) and elderly people found indoors are mostly exposed 4 . By 2030 when COPD will rank the third commonest cause of death, 54% of Indian population and 52% of other Asian countries will be still using traditional biomass fuels. 5. The World Health Organisation estimates that outdoor air pollution accounts for 5% of deaths worldwide, 1.3 million deaths per year and indoor air pollution contributes to 2 million premature deaths with more than 99% occurring in developing countries 6 . Of these, 44% are due to pneumonia, 54% from COPD and 2% from lung cancer. Biomass fuel attributes to 5-6% of the national burden of disease in India 7 and tops in the fuel related deaths in South Asian region Component of Biomass Fuel SmokeWorldwide wood is the most commonly used biomass fuel. More than 2 billion kg of biomass is burnt everyday in open fires and inefficient cookstoves 10 . The biomass smoke also contributes to outdoor air pollution. Wood smoke released during incomplete combustion is a complex mixture of various volatile and respirable particulate substances derived from wood polymers and resins. More than 200 chemicals and compound groups are identified and >90% of these are in the respirable size range 11 . Biomass smoke constituents are known to be toxic or have irritant effect on respiratory tract. They include particulate matter < 10 microns in aerodynamic diameter (PM10) and 2.5 microns (PM 2.5), carbon monoxide (CO), nitrogen dioxide, sulfur dioxide, aldehydes (formaldehyde), polycyclic aromatic hydrocarbons (benzopyrene), volatile organic compounds, chlorinated dioxins and free radicals. When coal is used, sulfur, arsenic and fluorine may also be present in the smoke.Amongst these PM10 has significant adverse health effects and in homes using biomass fuel, the m...

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DNA damage in lymphocytes of rural Indian women exposed to biomass fuel smoke as assessed by the Comet assay

Cited by 44 publications

References 43 publications

Indoor air pollution and the lung in low- and medium-income countries

Indoor air pollution and the lung in low- and medium-income countries

Effect of cigarette smoking on DNA damage according to nine comet assay parameters in female and male groups

Household Air pollution due to Biomass smoke exposure and Chronic Obstructive Pulmonary Disease

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