Comparative studies of RNA synthesis in two strains of cannibal giant <i>Blepharisma</i>

Bhandary, A. Vasanthi; Hirshfield, Henry I.

doi:10.1002/jcp.1030630212

Cited by 8 publications

(4 citation statements)

References 12 publications

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“…wardsi. This ciliate was described as perhaps an obligate carnivore, prone to conjugate, having 4-6 macronuclear nodes, with Feulgen-negative sites in the nodes of the giants but absent in the vegetative forms (4,7). B .…”

Section: Discussionmentioning

confidence: 99%

Macronuclear Variation During the Life Cycle of Blepharisma dawsoni n.sp. and B. wardsi

Christie

Hirshfield

1967

The Journal of Protozoology

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Section: Discussionmentioning

confidence: 99%

Macronuclear Variation During the Life Cycle of Blepharisma dawsoni n.sp. and B. wardsi

Christie

Hirshfield

1967

The Journal of Protozoology

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“…Cannibalism has been reported in other cell types, including human cancer cells. 64,65 Although cannibalism in single-cell protozoa, 15,[66][67][68] including Stentor, has been reported in the literature, the conditions leading to cannibalism in Stentor are not well understood. 15,68,69 It is known, however, that starvation does not drive cannibalism in Stentor.…”

Section: Asymmetric Splitting Of Cellsmentioning

confidence: 99%

Hydrodynamic dissection ofStentor coeruleusin a microfluidic cross junction

et al. 2022

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“…Cannibalism has been reported in other cell types, including human cancer cells. 54,55 Although cannibalism in single-cell protozoa, 15,[56][57][58] including Stentor, has been reported in the literature, the conditions leading to cannibalism in Stentor are not well understood. 15,58,59 It is known, however, that starvation does not drive cannibalism in Stentor.…”

Section: Asymmetric Splitting Of Cellsmentioning

confidence: 99%

Hydrodynamic dissection of single cells in a microfluidic system

Paul

Zhang

Jalil

et al. 2022

Preprint

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Stentor coeruleus, a single-cell ciliated protozoan, is a model organism for wound healing and regeneration studies. Despite Stentor's large size (up to 2 mm in extended state), microdissection of Stentor remains challenging. In this work, we describe a hydrodynamic cell splitter, consisting of a cross junction, capable of splitting Stentor cells in a non-contact manner at a high throughput of ~500 cells/min under continuous operation. Introduction of asymmetry in the flow field at the cross junction leads to asymmetric splitting of the cells to generate cell fragments as small as ~8.5 times the original cell size. Characterization of cell fragment viability shows reduced 5-day survival as fragment size decreases and as the extent of hydrodynamic stress imposed on the fragments increases. Our results suggest that cell fragment size and composition, as well as mechanical stress, play important roles in the long-term repair of Stentor cells and warrant further investigations. Nevertheless, the hydrodynamic splitter can be useful for studying phenomena immediately after cell splitting, such as the closure of wounds in the plasma membrane which occurs on the order of 100- 1000 seconds in Stentor.

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Comparative studies of RNA synthesis in two strains of cannibal giant Blepharisma

Cited by 8 publications

References 12 publications

Macronuclear Variation During the Life Cycle of Blepharisma dawsoni n.sp. and B. wardsi

Macronuclear Variation During the Life Cycle of Blepharisma dawsoni n.sp. and B. wardsi

Hydrodynamic dissection ofStentor coeruleusin a microfluidic cross junction

Hydrodynamic dissection of single cells in a microfluidic system

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