Comparative studies of sheep immunoglobulins IgG<sub>1</sub> and IgG<sub>2</sub>: Amino acid sequence of carboxy‐terminal cyanogen bromide fragments from their heavy chains

Conde, Francisco P.; Deverson, Edward V.; Milstein, C.

doi:10.1002/eji.1830050416

Cited by 7 publications

(3 citation statements)

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“…Variance analysis of LN of the values has been carried out, the relative error was found to be 6.9 per cent in assays with the laser nephelometer and 7.3 per cent in assays with the single radial immunodiffusion test. Conde et al, 1975;Verdouw-Chamalaun et al, 1977;Ciupercescu, 1977), although it is known (Bokhout, 1975 a,b) that adequate reproducibility is only attainable in radial immunodiffusion when…”

Section: Resultsmentioning

confidence: 99%

Quantitation of immunoglobulins in ovine sera and secretions by laser nephelometry. Comparison with the radial immunodiffusion (RID) technique

Goudswaard

Verdouw-Chamalaun

Noordzij

1980

Veterinary Immunology and Immunopathology

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In twenty-five ovine body fluids (serum, lung fluid and cerebrospinal fluid), the concentrations of IgGl, IgG2, IgM and IgA were determined by laser nephelometry and radial immunodiffusion (RID). When nephelometric assays are carried out, antisera free from any turbidity are essential. Methods ensuring that goat and rabbit antisera will satisfy this requirement are described in the present paper. When sheep immunoglobulins were measured by laser nephelometry, adequate and reproducible results were obtained, comparable with those obtained by RID.Advantages of the nephelometric method include the speed of assay and its sensitivity, allowing precise determination of the very low concentrations of immunoglobulins in CSF.

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Section: Resultsmentioning

confidence: 99%

Quantitation of immunoglobulins in ovine sera and secretions by laser nephelometry. Comparison with the radial immunodiffusion (RID) technique

Goudswaard

Verdouw-Chamalaun

Noordzij

1980

Veterinary Immunology and Immunopathology

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“…This relationship is more marked in the sequence of the CH2 subunit (K. Adetugbo and C. Milstein, in preparation). This may mean that the divergence of mouse y l and y2a subclasses, unlike in human y [40,3], bovine [41], horse [42] and sheep y [43] subclasses, is not a recent event, and occurred before the divergence of man and mouse. It is likely that the divergence between mouse y2a and y1 preceded the divergence of mouse y l and human y (i.e.…”

Section: K V S L T C M I T D F F P E D I T V E W E S N G Q a P E N mentioning

confidence: 99%

“…For (in the mouse) y l and y2a proteins, which apparently diverged before speciation, are not y subclasses in the same sense as human y l and y4 which diverged very recently. The close sequence similarity between the human y subclasses is seen between mouse y2a and ;~2b when the sequences around the disulphide bridges are compared (C. Milstein, unpublished), and between the selected sequences in the y subclasses of sheep [43], cow [41] and horse [42]. So, it is probably correct to refer to the mouse y2a and y2b as subclasses of y2.…”

Section: K V S L T C M I T D F F P E D I T V E W E S N G Q a P E N mentioning

confidence: 99%

Mouse Immunoglobulin Subclasses: Cyanogen Bromide Fragments and Partial Sequence of a gamma1 Chain

Adetugbo¹,

Milstein²,

Poskus³

et al. 1975

Eur J Biochem

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The purification and characterisation of all the cyanogen bromide fragments of MOPC 21 heavy (yl) chain is described. The ten BrCN fragments account for the whole chain. Four of these fragments have been used to establish the sequence of the C-terminal stretch (138 residues) that includes the entire CH3 and a part of the CH2 homology regions. A comparison of this sequence with homologous sequences is presented. Mouse y l and y2 proteins differ much more than the y subclasses in humans and in other species. The comparison further suggests that the four human y subclasses and mouse y l have a common ancestor which differs from the mouse y 2 ancestor. Unlike other subclasses, mouse y l and y 2 genes have diverged before speciation.Heavy and light chains of immunoglobulins contain an amino-terminal variable portion (the V-region) of 100 -120 residues, and a carboxy-terminal "constant" portion (the C-region) which includes the remainder of the chain. All the heavy chains share a common pool of V-region genes while a cluster of C-region tandem genes defines the variety of classes and subclasses of heavy chains. (For a recent review, see [l] and [2].)Comparative sequence studies, especially of human IgG subclasses, led to the suggestion that subclasses resulted from recent tandem gene duplications and that the genes were under a constant process of expansion and contraction [3]. Clearly, more extensive studies were required to test the generality of these observations. Mouse immunoglobulins are particularly useful for such studies because of the availability of myeloma proteins [4]. Murine immunoglobulins include three major immunoglobulin G subclasses which differ in their interchain bridges [5,6]. Sequences around their intrachain bridges are also available [6,5], and extensive sequence studies on a murine y2a protein (MOPC 173 H-chain) have been reportedThe work described here was done on the mouse plasmacytoma MOPC 21 which contains a y l (heavy) chain. Specific interest in this chain derives from the [7,81.

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Studies on the lymphocytes of sheep. Destination of lymph‐borne immunoblasts in relation to their tissue of origin

1977

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Lymph-borne immunoblasts were labeled in vitro with 125I[]iodo-deoxy-uridine, washed and returned by intravenous injection to the sheep from which they had been collected. Twenty h later the sheep were killed and the distribution of the immunoblasts was determined by assaying the radioactivity in various organs. Immunoblasts from the efferent lymph of peripheral somatic lymph nodes (PSLN) went mainly to the spleen, lungs and other PSLN, while immunoblasts from intestinal lymph went mainly to the small gut. This ability of intestinal immunoblasts to home to the gut was demonstrated also in the sterile environment of fetuses in utero; apparently the migratory behavior of immunoblasts, like that of small lymphocytes, is not primarily "antigen-driven". A technique was devised for the collection of peripheral (i.e. afferent to the mesenteric node) intestinal lymph which was found to contain 10-20 times the numbers of small lymphocytes that occur in the peripheral lymph from other tissues. Immunoblasts from peripheral intestinal lymph also homed to the gut. The immunoglobulin content of immunoblasts was studied by making detergent extracts of lymph cells, by applying immuno-peroxidase techniques to cell films and by investigating the incorporation of 14C-labeled amino acids into immunoglobulins by immunoblasts in vitro. Immunoblasts from both somatic and intestinal lymph contained and made IgG and IgM, but many intestinal immunoblasts contained and made IgA. It is not known whether this immunoglobulin mediates the extravasation of immunoblasts into the gut. Nonetheless, there is compelling evidence that there are two major migratory pathways for lymphoid cells; one through the gut-associated lymphoid tissue and the other through the somatic-splenic lymphoid tissues.

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Comparative studies of sheep immunoglobulins IgG₁ and IgG₂: Amino acid sequence of carboxy‐terminal cyanogen bromide fragments from their heavy chains

Cited by 7 publications

References 20 publications

Quantitation of immunoglobulins in ovine sera and secretions by laser nephelometry. Comparison with the radial immunodiffusion (RID) technique

Quantitation of immunoglobulins in ovine sera and secretions by laser nephelometry. Comparison with the radial immunodiffusion (RID) technique

Mouse Immunoglobulin Subclasses: Cyanogen Bromide Fragments and Partial Sequence of a gamma1 Chain

Studies on the lymphocytes of sheep. Destination of lymph‐borne immunoblasts in relation to their tissue of origin

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Comparative studies of sheep immunoglobulins IgG1 and IgG2: Amino acid sequence of carboxy‐terminal cyanogen bromide fragments from their heavy chains

Cited by 7 publications

References 20 publications

Quantitation of immunoglobulins in ovine sera and secretions by laser nephelometry. Comparison with the radial immunodiffusion (RID) technique

Quantitation of immunoglobulins in ovine sera and secretions by laser nephelometry. Comparison with the radial immunodiffusion (RID) technique

Mouse Immunoglobulin Subclasses: Cyanogen Bromide Fragments and Partial Sequence of a gamma1 Chain

Studies on the lymphocytes of sheep. Destination of lymph‐borne immunoblasts in relation to their tissue of origin

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Comparative studies of sheep immunoglobulins IgG₁ and IgG₂: Amino acid sequence of carboxy‐terminal cyanogen bromide fragments from their heavy chains