Comparative studies on the structure, biological activity and molecular mechanisms of polysaccharides from Craterellus cornucopioide (CC-M) and Dictyophora indusiata (Vent.ex Pers) Fisch (DI-Z)

Abstract

In this study, two new polysaccharides were extracted from the fruiting bodies of Craterellus cornucopioide and Dictyophora indusiata (Vent.ex Pers) Fisch, respectively. The results showed that CC-M was composed of Xyl: Glc: Gal in a ratio of 2:5:4. The CC-M takes 1,6-glucose and 1,6-galactose as skeletons, extends a branched chain from galactose 2-O to connect 1,3,4-xylose, and connects β-4-glucose terminal monosaccharide to xylose. DI-Z was also composed of Xyl: Glc: Gal in the ratio of 2:6:5, which takes 1,6-glucose and 1,6-galactose as skeletons, extends a branched chain from galactose 2-O to connect 1,4-xylose and β-4-glucose as terminal group. The results of immunoactivity showed that CC-M and DI-Z have the proliferation activity of B cells, T cells and RAW264.7 cells in vitro, and the effect of CC-M on the proliferation of immune cells was higher than that of DI-Z. In addition, CC-M could significantly promote the secretion of TNF -α, IL-6, IL-1 β, IL-10 and CD163 and enhance the phagocytic ability of RAW264.7 cells. The results of RNA-sequencing showed that CC-M exerted its proliferative activity and immunoregulatory activity on RAW264.7 cells through the interaction of MAPK signaling pathway, NOD like receptor signaling pathway, PI3K-Akt signaling pathway and NF-κB signaling pathway.

Black Trumpet [Craterellus cornucopioides (L.) Pers.]—Bioactive Properties and Prospects for Application in Medicine and Production of Health-Promoting Food

“…They have the ability to stimulate the functioning of the immune system in mice by stimulating the activity of RAW264.7 macrophages. Ding et al [60] came to similar conclusions as a result of examining the bioactivity of the CC-M polysaccharide isolated from C. cornucopioides. It increased the activity of T and B lymphocytes and RAW264.7 macrocytes and stimulated the signaling pathways of MAPK, PI3K-Akt, NF-κB, and NOD-like receptor signaling pathway receptors as a result of stimulating the secretion of CD163, IL-1β, IL-6, IL-10, and TNF-α.…”

Structure, function and mechanism of edible fungus polysaccharides in human beings chronic diseases

“…Studies also have shown that there are certain differences in the amount and structural characteristics of polysaccharides contained in different edible fungi (Jing et al, 2021). For example, Craterellus cornucopioide polysaccharides and Dictyophora indusiata (Vent.ex Pers) Fisch polysaccharides with similar monosaccharide composition had proliferative activity on specific immune cells such as B cells, T cells and RAW264.7 cells, but Craterellus cornucopioide polysaccharides had a better effect on immune cell proliferation than Dictyophora indusiata (Vent.ex Pers) Fisch polysaccharides (Ding et al, 2022b). The molecular weight of polysaccharides affects the biological activity of polysaccharides.…”

Characterization of Polysaccharide Extracts of Four Edible Mushrooms and Determination of In Vitro Antioxidant, Enzyme Inhibition and Anticancer Activities

“…26 In a different study on C. cornucopioides polysaccharide extract, the monosaccharide composition was detected as xylose: glucose: galactose with a 2:5:4 molar ratio. 27 2.3. FT-IR Analysis.…”