Comparative Studies on the Structure and Properties of Two Selected Strains of Japanese Encephalitis Virus

Nishimura, Chiaki; Nomura, Masako; Kitaoka, Masami

doi:10.7883/yoken1952.21.1

Cited by 6 publications

(2 citation statements)

References 8 publications

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“…Flaviviruses are small, enveloped viruses with approximately 500 Å in diameter with icosahedral symmetry (Murphy et al, 1968;Nishimura et al, 1968;(Mukhopadhyay et al, 2003). The virion contains a positive-sense, single stranded RNA genome of approximately 11 kilobases (kb) in length and lacks a 3' polyadenylate tail with type I methylated cap, m 7 GpppAmpN1 at the 5' end (Stollar et al, 1967;Wengler and Gross, 1978;Cleaves and Dubin, 1979).…”

Section: Introductionmentioning

confidence: 99%

The implication of amino acid mutations at flavivirus NS1-2A cleavage site on NS1’ protein production

Bettadapura¹,

Addis²

2015

MJM

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Aims:The presence of a C-terminally extended form of NS1 (NS1' protein) has been previously reported in encephalitic flaviviruses, due to the presence of -1 programmed ribosomal frameshift at the N-terminal of NS2A protein. This present study is aimed to further confirm that the NS1' protein production is independent of the authentic cleavage at NS1-2A junction. Methodology and results:Six different constructs (P1-Leu, P2-Asp, P3-Phe, P3-Leu, P3-Gly and P5-8 Ala) containing various mutations at conserved and variable amino acids at C-terminal of NS1 protein were generated by site-directed mutagenesis and analysed with transient polyprotein expression assay. While analysis on the NS1-2A cleavage of the mutants exhibited extremely poor to efficient cleavage ranging from 6-89%, significant amount of NS1' being expressed in all mutants irrespective of their NS1-2A cleavage outcome. Conclusion, significance and impact study: In this analysis, we showed for the first time that the abolishment of the authentic NS1-2A cleavage in Murray Valley encephalitis virus (MVEV) did not impact on NS1' production. This observation extend on previous studies to show that NS1 and NS2A proteins are the product of NS1-2A cleavage which is catalysed by an unknown host protease while NS1' protein is a product of ribosomal frameshift, independent of the authentic cleavage at NS1-2A junction.

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Section: Introductionmentioning

confidence: 99%

The implication of amino acid mutations at flavivirus NS1-2A cleavage site on NS1’ protein production

Bettadapura¹,

Addis²

2015

MJM

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“…Proc., p. 162,1969). Lipid composition of group B arboviruses has not been fully examined by direct analysis of purified virus particles (27). Grossberg and O'Leary and Grossberg et al showed occurrence of hyperlipemia in chicken embryos after JEV infection (14,15).…”

mentioning

confidence: 99%

Effect of fatty acids on growth of Japanese encephalitis virus cultivated in BHK-21 cells and phospholipid metabolism of the infected cells

Makino¹,

Jenkin²

1975

J Virol

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Growth of Japanese encephalitis virus (JEV) in BHK-21 cells was stimulated in the presence of 20 to 40 ug of the sodium salt of oleic acid (cis-9-octadecenoic acid, 9-18:1) per ml supplemented in Waymouth medium. The stimulatory effect of the salt was highest when 9-18:1 was added after adsorption of the virus. Study of the effect of other fatty acids on growth of JEV showed the following results: the longer the chain length of the saturated fatty acid salt, the higher the stimulatory effect on viral growth. In contrast, polyunsaturated fatty acids had an inhibitory effect on viral growth. The effect of isomeric cis-octadecenoic acids on viral growth was variable, depending upon the position of the double bond. The cis-6-octadecenoic acid had the highest inhibitory effect on growth of JEV compared to other isomeric octadecenoic acids. The sodium salt of [1-'IC ]cis-9octadecenoic acid (9-18:1, 20 gg/ml) was rapidly incorporated into control and JEV-infected cells. Specific radioactivity in phosphatidylcholine dropped 12 to 24 h after virus inoculation, whereas synthesis of phosphatidylethanolamine increased 12 to 24 h after virus inoculation in infected cells compared to uninfected cells. Results from these studies suggest that phospholipid metabolism of infected cells is markedly changed, which can be associated with altered fatty acid metabolism when using labeled 9-18:1 fatty acid as a marker.

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Togavirus Morphology and Morphogenesis

Murphy¹

1980

The Togaviruses

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Comparative Studies on the Structure and Properties of Two Selected Strains of Japanese Encephalitis Virus

Cited by 6 publications

References 8 publications

The implication of amino acid mutations at flavivirus NS1-2A cleavage site on NS1’ protein production

The implication of amino acid mutations at flavivirus NS1-2A cleavage site on NS1’ protein production

Effect of fatty acids on growth of Japanese encephalitis virus cultivated in BHK-21 cells and phospholipid metabolism of the infected cells

Togavirus Morphology and Morphogenesis

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