Comparative Studies on Vegetative and in vitro Propagation of Elite Selected Jojoba Strains

Abstract

Aims: Evaluate the responses of five jojoba genotypes to in vitro nodal segments and shoot tip culture under different growth regulators combinations. Study Design: Comparative evaluation between vegetative and in vitro propagation of jojoba genotype. Place and Duration:The study was carried out in tissue culture lab of fruit breeding department during 2015. Methodology: Five selected elite genotypes of jojoba symbolled by 'C10, C16, C18, C19 and C21', and cultivated in the Cairo-Alexandria desert road were propagated asexually via cutting and Original Research Article

Review Article Propagation of Jojoba, Simmondsia chinensis (Link) Schnneider: Constraints and Prospects

“…It is reported that perlite along with vermiculite proven best media for jojoba cuttings as, growth related parameters such as length, rate of survival, roots number per cutting, diameter, and number of leaves per cutting observed higher as compared to other growing medias [34]. Jojoba cuttings treated with different concentrations i.e., 1500, 2500 and 3000 mg/L of IBA for propagating different genotypes of jojoba utilizing growth media of equal composition of vermiculite, perlite, and peat moss and recorded success to initiate roots from only 3000 mg/L [77]. It remained a concern that saplings planted in field conditions would not perform better than seedlings.…”

“…MS media containing 2.0 mg/L BAP yields maximum (9.13) number of shoots when different levels (.5, 2 and 2.5 mg/L) KIN, BAP and TDZ (0.22,0.44 and 0.88) mg/L were combined by using with NAA, IBA and IAA @ 1.25 mg/L) [27]. Significant results for maximum division, number of shoots, leaves and length of shoot was observed by combined application of 3 and .5 mg/L of BA and NAA, respectively on 60 days old tips of shoots and semi-soft wood cuttings from a 6-month-old plant of 5 genotypes of jojoba when cultured on various levels of plant growth regulators [77]. Axillary bud of a nodal segment taken from a male or female plant to proliferate in a modified Schenk and Hildebrandt's (SH) medium supplemented with 1 mg /L each of BAP and IAA and obtained 5-8 shoots per explant of single-nodes in initial cultures were induced, but the number of shoots in subsequent cultures increased substantially to about 15 in low BAP (0.5 mg/L) containing medium [106,107,108,109,110].…”

“…30% success was gained by using 1.0mg/L IBA and 40% by utilization of 3.0 mg/L in combination with MS media [27]. However, failure was recorded in five jojoba genotypes in initiation of roots as well as in proliferated plants when various levels 0.5, 1 and 2mg/L of BAP, NAA and TDZ were used as treatment [77]. Difficulties observed for acclimatization when micro propagates of jojoba are required to transplant in field [56,79,110], significant mortality of rooting system was also reported when provided with high humidity and reduction in roots aeration [110], as small sized leaves generated during culture [79].…”

Evaluation of the Effect of Elite Jojoba Lines on the Chemical Properties of their Seed Oil

“…Hence, the main aim of this study was to evaluate the effect of genetic variability on the chemical composition of Egyptian jojoba seeds and the potential variations in the chemical characteristics of JO of elite female jojoba lines selected by Nahla et al [22,23]. In this respect, the fatty acidcomposition, iodine number, and peroxide value of JO were determined to investigate the significant differences amongelite jojoba lines.…”