Comparative study between using Lowenstein Jensen, Bio-FM media and mycobacteria growth indicator tube (MGIT) system in identification of Mycobacterium tuberculosis

Essawy, Tarek S.; Saeed, Amal; Fouad, Nehad A

doi:10.1016/j.ejcdt.2014.01.001

Cited by 6 publications

(4 citation statements)

References 14 publications

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“…However, culture on it is long (3 weeks to 3 months for M. tuberculosis) and time-consuming. MGIT was better than LJ medium in recovery rate and detection time of mycobacterial growth [20]. Due to its sensitivity, MGIT should be used for smear-negative pulmonary TB patients [21].…”

Section: Discussionmentioning

confidence: 95%

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Antituberculosis Activity of Extract and Fractions of Tinospora Crispa Against Mycobacterium Tuberculosis H37rv Using Mycobacteria Growth Indicator Tube and Agar Proportion Method

Wahyuningrum

Ritmaleni

Irianti

et al. 2018

Asian J Pharm Clin Res

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Objective: The increasing incidence of multidrug-resistant tuberculosis (TB) has created a need to discover a new anti-TB drug candidates. The aim of this study was to screen extract and fractions of Tinospora crispa for activity against Mycobacterium tuberculosis H37Rv.Methods: The dried and pulverized T. crispa stem was extracted by maceration method using ethanol (96%). The anti-TB activity was carried out using mycobacteria growth indicator tube (MGIT) system and agar proportion method with Lowenstein–Jensen (LJ) medium.Result: The result of this study showed that ethanolic extract and fractions of T. Crispa did not exhibit anti-TB activity in the range of 100–1000 μg/ml with MGIT method, while with agar proportion method, there were M. tuberculosis colonies growth on the LJ containing 1000 μg/ml extract slants.Conclusion: The tested extract and fractions of T. crispa have no anti-TB activity against M. tuberculosis until 1000 μg/ml.

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Section: Discussionmentioning

confidence: 95%

“…During bacterial growth within the tube, the free oxygen is used and is replaced with carbon dioxide. The decrease of free oxygen made the fluorochrome was no longer inhibited and produces a fluorescence within the MGIT tube when visualized under UV light [20].…”

Section: Discussionmentioning

confidence: 99%

Antituberculosis Activity of Extract and Fractions of Tinospora Crispa Against Mycobacterium Tuberculosis H37rv Using Mycobacteria Growth Indicator Tube and Agar Proportion Method

Wahyuningrum

Ritmaleni

Irianti

et al. 2018

Asian J Pharm Clin Res

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“…LJ medium includes very high concentrations of malachite green which helps to prevent contamination with other species of bacteria. Mycobacterium Growth Indicator Tube was better media as compared to LJ medium in retrieval rate of Mycobacterial growth [24] . MGIT should be used mostly for assessing antituberculosis effect of different agents.…”

Section: Introductionmentioning

confidence: 91%

Effect of ethanomedicinal plants on Mycobacterium smegmatis Tubercular bacilli using mycobacteria growth indicator tube assay

Vaghela¹,

Bhatt²,

Zaveri³

2023

Int. J. Herb. Med.

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Despite all of the control approaches, tuberculosis (TB) is a major cause of death worldwide and onethird of the world's population is infected with TB. Plant-derived medicines have been used in traditional medicinal system for the treatment of many ailments worldwide. From last number of years, plants have advantageous in different type of diseases producing in human beings. The present aim to carry out the evaluation of the antimycobacterial activity of selected eleven medicinal plants. Three different extracts were prepared and evaluated for its antimycobacterial activity against Mycobacterium smegmatis using Mycobacterial Growth Indicator Tube (MGIT) assay. The MGIT assay consists of liquid broth medium that is known to yield better recovery and faster growth of mycobacteria. Isoniazid was used as standard antituberculosis drug. The percentage for anti-mycobacterial smegmatis activity among tested eleven medicinal plants, aqueous extract of Oscimum sanctum, Adhatoda vasica, Leptadenia reticulata and Cocculus hirsutus shows good antimycobacterial activity. Among these four Leptadenia reticulata and Cocculus hirsutus shows potent inhibition as compared to isoniazid. Thus, its result supports the uses of these plants in traditional medicine and also helps to cure and prevent tuberculosis. It can further have studied using more specific methods for antimycobacterial activity.

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“…The decrease of free oxygen produces a fluorescence within the MGIT tube when visualized under UV light. 19 MGIT is now considered to be the standard method and is widely used. The mean time for MTB detection is 12.9 days with BACTECMGIT 960 and 15 days with BACTEC 460.…”

Section: Mycobacterial Growth Indicator Tube Systemmentioning

confidence: 99%

Diagnosis of Childhood Tuberculosis

Shah¹,

Shah²

2019

Pediatric Infectious Disease

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Tuberculosis (TB) in India accounts for about a quarter of the world's TB cases and children are at high risk of tuberculous infection and TB disease. TB diagnosis in children continues to be a challenge due to difficulty in getting appropriate samples and paucibacillary disease. In recently revised recommendations a major emphasis has been made on microbial diagnosis and more liberal use of molecular diagnostic modalities like cartridge-based nucleic acid amplification test (CBNAAT) and line probe assay. Cartridge based nucleic acid amplification test (CB-NAAT, GeneXpert,) is now considered as a World Health Organization (WHO)-recommended rapid diagnostic (WRD ) and is a very simple test ,with a well-established role in the diagnosis of pulmonary tuberculosis (PTB) and extrapulmonary tuberculosis (EPTB) cases in comparison to AFB smear. CBNAAT should be offered upfront whereever TB is suspected. Drug sensitivity tests (DST) should always be attempted in all positive cases. For latent TB infection diagnosis intradermal TST is widely used however interferon-gamma release assays is also an equivalent option.

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Comparative study between using Lowenstein Jensen, Bio-FM media and mycobacteria growth indicator tube (MGIT) system in identification of Mycobacterium tuberculosis

Cited by 6 publications

References 14 publications

Antituberculosis Activity of Extract and Fractions of Tinospora Crispa Against Mycobacterium Tuberculosis H37rv Using Mycobacteria Growth Indicator Tube and Agar Proportion Method

Antituberculosis Activity of Extract and Fractions of Tinospora Crispa Against Mycobacterium Tuberculosis H37rv Using Mycobacteria Growth Indicator Tube and Agar Proportion Method

Effect of ethanomedicinal plants on Mycobacterium smegmatis Tubercular bacilli using mycobacteria growth indicator tube assay

Diagnosis of Childhood Tuberculosis

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