Comparative study for safety and efficacy of a novel folic acid formulation gel with 0.1% triamcinolone acetonide paste in experimentally induced oral ulcer

Abstract

Objectives: The objective of the present study was to evaluate the efficacy and safety of 0.1% novel folic acid gel in blunt dental instrument-induced oral ulcer in animal models (both rats and rabbits). Materials and Methods: The study was conducted in the Experimental Laboratory, Department of Pharmacology, Jawaharlal Nehru Medical College, Aligarh Muslim University. Animals were randomly divided into three interventional groups, namely Group 1 (folic acid gel), Group 2 (triamcinolone oromucosal paste) and Group 3 (control). We created a linear oral ulcer of 5 mm with the blunt dental instrument in both rats and rabbits. Each group consists of 10 animals (6 rats + 4 rabbits). Group 1 received test drug 0.1% novel folic acid gel formulated by us, Group 2 was positive control and received standard local drug 0.1% Kenacort triamcinolone oromucosal paste and Group 3 served as a negative control group where no treatment was given. Results: No untoward reaction was observed on the irritancy test. Kruskal–Wallis test applied for comparison of results. After 1 week of post-ulcer induction, there was a marked improvement in healing of oral ulcers in both Group 1 and Group 2, but more statistically significant healing was seen in Group 1 (folic acid gel). Both Groups 1 and 2 showed steady improvement in the healing of oral ulcers as compared to their pre-treated values and again were more marked in Group 1. Conclusion: Local folic acid gel supplementation was safe and had shown to augment oral ulcer healing in rats and rabbit’s animal models.

In‐vivo assessment of application of folinic acid and botulinum toxin A in cleft lip surgical defects

“…Additional to the fibroblast proliferation and collagen deposition, folinic acid was also effective in epithelial healing and neo‐vascularization. The effects of a synthetic folic acid gel has also been shown in acceleration of wound healing in oral ulcers in animal models 36 . Additionally, in a study conducted by Duman et al.…”

“…The effects of a synthetic folic acid gel has also been shown in acceleration of wound healing in oral ulcers in animal models. 36 Additionally, in a study conducted by Duman et al 2.5% folinic acid enhanced wound healing by re-epithelialization, neo-vessel formation, inflammatory cell migration, and collagen deposition. 20 The present study is bound to the limitations of in-vivo experimental animal studies.…”

Histologic Evaluation of the Effects of Folinic Acid Chitosan Hydrogel and Botulinum Toxin A on Wound Repair of Cleft Lip Surgery in Rats

“…In a study performed by Duman et al, the topical application of 2.5% folinic acid in rat animal models has caused an increase in fibroblastic proliferation and collagen deposition leading to accelerated wound healing [11]. Additionally, a synthetic folic acid gel showed enhanced wound repair in oral ulcers of rats and rabbits [19]. Nonetheless, excessive fibroblast proliferation and the resultant collagen accumulation lead to the formation of hypertrophic scar.…”