Comparative study of antioxidants as quenchers or scavengers of reactive oxygen species based on quenching of MCLA-dependent chemiluminescence

Hosaka, Shuntaro; Obuki, M; Nakajima, Jun; Suzuki, Mitsuko

doi:10.1002/bio.867

Cited by 39 publications

(27 citation statements)

References 36 publications

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“…Singlet oxygen was generated by light irradiation of a rose Bengal solution in a test tube, and detected by chemiluminescence of MCLA 17,18. The suspension of Asx‐EPC‐lipo (0.1 mL) was diluted with 0.879 mL of deionized distilled water, followed by the addition of 20 µL of 0.5 mM rose Bengal solved with deionized distilled water.…”

Section: Methodsmentioning

confidence: 99%

Protective Effects of Topical Application of a Poorly Soluble Antioxidant Astaxanthin Liposomal Formulation on Ultraviolet-Induced Skin Damage

Hama

Takahashi

Inai

et al. 2012

Journal of Pharmaceutical Sciences

113

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Section: Methodsmentioning

confidence: 99%

Protective Effects of Topical Application of a Poorly Soluble Antioxidant Astaxanthin Liposomal Formulation on Ultraviolet-Induced Skin Damage

Hama

Takahashi

Inai

et al. 2012

Journal of Pharmaceutical Sciences

113

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“…L-Ascorbic acid was purchased from Sigma-Aldrich Japan K.K. (11). Catechin hydrate was purchased from Spectrum Chemical Manufacturing Corp. (Gardena, CA; refs.…”

Section: Methodsmentioning

confidence: 99%

Monitoring of Singlet Oxygen Is Useful for Predicting the Photodynamic Effects in the Treatment for Experimental Glioma

Yamamoto¹,

Yamamoto

Hirano

et al. 2006

Clinical Cancer Research

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O 2 signal gradually decreased with a low peak, whereas at a high fluence rate it decreased immediately with a high peak. Consequently, the cumulative 1 O 2 at a low fluence rate was higher, which thus induced a strong photodynamic effect. The proportion of apoptosis to necrosis might therefore be dependent on the peak and duration of the 1 O 2 signal. A low fluence rate tended to induce apoptotic change, whereas a high fluence rate tended to induce necrotic change. Conclusions:The results of this study suggested that the monitoring of 1 O 2 enables us to predict the photodynamic effect, allowing us to select the optimal laser conditions for each patient.

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“…The strong CL phenomenon indicated that ROS was released on CaO 2 . In order to acquire sufficient evidence, ascorbic acid, a well-known ROS scavenger (22)(23)(24), was chosen for further experiments. As shown in Fig.…”

Section: Confirmation Of Ros On Cao 2 By CLmentioning

confidence: 99%

Study on the generation mechanism of reactive oxygen species on calcium peroxide by chemiluminescence and UV‐visible spectra

Zhang

Zhao

et al. 2007

Luminescence

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In the present work, the generation mechanism of reactive oxygen species (ROS) on calcium peroxide (CaO 2 ) was studied. A very intense chemiluminescence (CL) signal was observed when adding an aqueous solution of luminol or 2-methyl-6-(4-methoxyphenyl)-3,7-dihydroimidazo[1,2α]-pyrazin-3-one hydrochloride (MCLA) to a suspension of CaO 2 . The ROS released on CaO 2 were thought to be oxidizing agents leading to CL, and were characterized by CL, UV-visible (UV-vis) spectra and the effective scavengers of the special ROS. ( 1 O 2 ) in the process. Obviously, the release of the ROS on CaO 2 is the main factor that contributed to the effect of CaO 2 , but the details have not been clarified and needed further research.Chemiluminescence (CL) is a powerful analytical technique that has excellent sensitivity, and a wide linear dynamic range and requires relatively simple and inexpensive instrumentation. Furthermore, CL is an effective method in research on ROS, because of the associated instantaneous nature of CL and the short lifetime of ROS radicals (11-16). Goto et al. (17) successfully investigated the CL mechanism of the alkalineearth metal peroxides (MgO 2 , BaO 2 ) with CL reagents (luminol and lucigenin), and demonstrated that the hydroxyl ( • OH) and • O 2 − were released chiefly on the MgO 2 and BaO 2 , respectively, with CL. According to our literature search, corresponding reports about CaO 2 have not so far been made.

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Comparative study of antioxidants as quenchers or scavengers of reactive oxygen species based on quenching of MCLA-dependent chemiluminescence

Cited by 39 publications

References 36 publications

Protective Effects of Topical Application of a Poorly Soluble Antioxidant Astaxanthin Liposomal Formulation on Ultraviolet-Induced Skin Damage

Protective Effects of Topical Application of a Poorly Soluble Antioxidant Astaxanthin Liposomal Formulation on Ultraviolet-Induced Skin Damage

Monitoring of Singlet Oxygen Is Useful for Predicting the Photodynamic Effects in the Treatment for Experimental Glioma

Study on the generation mechanism of reactive oxygen species on calcium peroxide by chemiluminescence and UV‐visible spectra

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