Comparative study of cholinergic cells in retinas of various mouse strains

Kang, Tae-Hoon; Ryu, Young Bae; Kim, In‐Beom; Oh, Goo Taeg; Chun, Myung-Hoon

doi:10.1007/s00441-004-0907-5

Cited by 27 publications

(24 citation statements)

References 50 publications

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“…There are also reports that nAChR expression differs among mouse strains in other brain regions which includes an age-dependent component. For example, a substantial (2-fold) strain-specific variability in nAChR expression is observed in the striatum (Altavista et al, 1987) as well as the retina (Kang et al, 2004) and cerebellum (Turner and Kellar, 2005). Further, a dynamic relationship between nAChR expression by CA1 neurons and astrocytes has also been suggested to be found throughout the life span of both rodents and humans (Hosli et al, 2000;Graham et al, 2002;Graham et al, 2003;Teaktong et al, 2004;Gahring et al, 2005a).…”

Section: Discussionmentioning

confidence: 99%

Nicotinic acetylcholine receptor expression in the hippocampus of 27 mouse strains reveals novel inhibitory circuitry

Gahring

Rogers

2008

Hippocampus

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Mouse strains are well-characterized to exhibit differences in their physiological and behavioral responses to nicotine. This report examines the expression of the high-affinity nicotine binding receptor subunit, neuronal nicotinic receptor subunit alpha4 (nAChRα4), in the dorsal hippocampus of 27 inbred mouse strains. Multiple differences among mouse strains in the cellular expression of nAChRα4 between subregions of the hippocampal field are evident. Differences that we describe in the expression of nAChRα4 suggest mouse strains of diverse genetic origin could exhibit significant variation in how this receptor contributes to modulating intra-hippocampal circuitry. These findings define a genetic frame-work in which the strain-specific responses to nicotine include underlying contributions by the varied anatomical context in which nAChRs are expressed.

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Section: Discussionmentioning

confidence: 99%

Nicotinic acetylcholine receptor expression in the hippocampus of 27 mouse strains reveals novel inhibitory circuitry

Gahring

Rogers

2008

Hippocampus

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“…The immunogen for the ChAT affinity-purified antibody was human placental enzyme. The antibody has been characterized extensively in mouse retinal tissue (see Kang et al, 2004;Heinze et al, 2007), and has been shown to label an expected 68 -70-kD band in immunoblots (Brunelli et al, 2005). Retinas were then rinsed in PBS and incubated in donkey antigoat IgG conjugated to Cy2 (at 1:200; Jackson ImmunoResearch, West Grove, PA) overnight.…”

Section: Methodsmentioning

confidence: 99%

Spatial patterning of cholinergic amacrine cells in the mouse retina

Whitney

Keeley

Raven

et al. 2008

J of Comparative Neurology

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The two populations of cholinergic amacrine cells in the inner nuclear layer (INL) and the ganglion cell layer (GCL) differ in their spatial organization in the mouse retina, but the basis for this difference is not understood. The present investigation examined this issue in six strains of mice that differ in their number of cholinergic cells, addressing how the regularity, packing, and spacing of these cells varies as a function of strain, layer, and density. The number of cholinergic cells was lower in the GCL than in the INL in all six strains. The nearest neighbor and Voronoi domain regularity indexes as well as the packing factor were each consistently lower for the GCL. While these regularity indexes and the packing factor were largely stable across variation in density, the effective radius was inversely related to density for both the GCL and INL, being smaller and more variable in the GCL. Consequently, despite the lower densities in the GCL, neighboring cells were more likely to be positioned closer to one another than in the higher-density INL, thereby reducing regularity and packing. This difference in the spatial organization of cholinergic cells may be due to the cells in the GCL having been passively displaced by fascicles of optic axons and an expanding retinal vasculature during development. In support of this interpretation, we show such displacement of cholinergic somata relative to their dendritic stalks and a decline in packing efficiency and regularity during postnatal development that is more severe for the GCL.

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“…Rod and (putative) ONcone bipolar cells were immunostained with a mouse monoclonal antibody against G-protein Go␣ (Haverkamp and Wä ssle, 2000). Cholinergic amacrine cells were labeled with a goat polyclonal antibody against choline acetyltransferase (ChAT) (Jeon et al, 1998;Haverkamp and Wä ssle, 2000;Kang et al, 2004). RGCs including their dendrites and axons were immunolabeled with a mouse monoclonal antibody against tubulin, ␤III isoform (Sharma and Netland, 2007).…”

Section: Antibodiesmentioning

confidence: 99%

Wolfram syndrome 1 (Wfs1) gene expression in the normal mouse visual system

Kawano¹,

Tanizawa²,

Shinoda³

2008

J of Comparative Neurology

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Wolfram syndrome (OMIM 222300) is a neurodegenerative disorder defined by insulin-dependent diabetes mellitus and progressive optic atrophy. This syndrome has been attributed to mutations in the WFS1 gene, which codes for a putative multi-spanning membrane glycoprotein of the endoplasmic reticulum. The function of WFS1 (wolframin), the distribution of this protein in the mammalian visual system, and the pathogenesis of optic atrophy in Wolfram syndrome are unclear. In this study we made a detailed analysis of the distribution of Wfs1 mRNA and protein in the normal mouse visual system by using in situ hybridization and immunohistochemistry. The mRNA and protein were observed in the retina, optic nerve, and brain. In the retina, Wfs1 expression was strong in amacrine and Müller cells, and moderate in photoreceptors and horizontal cells. In addition, it was detectable in bipolar and retinal ganglion cells. Interestingly, moderate Wfs1 expression was seen in the optic nerve, particularly in astrocytes, while little Wfs1 was expressed in the optic chiasm or optic tract. In the brain, moderate Wfs1 expression was observed in the zonal, superficial gray, and intermediate gray layers of the superior colliculus, in the dorsomedial part of the suprachiasmatic nucleus, and in layer II of the primary and secondary visual cortices. Thus, Wfs1 mRNA and protein were widely distributed in the normal mouse visual system. This evidence may provide clues as to the physiological role of Wfs1 protein in the biology of vision, and help to explain the selective vulnerability of the optic nerve to WFS1 loss-of-function.

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Comparative study of cholinergic cells in retinas of various mouse strains

Cited by 27 publications

References 50 publications

Nicotinic acetylcholine receptor expression in the hippocampus of 27 mouse strains reveals novel inhibitory circuitry

Nicotinic acetylcholine receptor expression in the hippocampus of 27 mouse strains reveals novel inhibitory circuitry

Spatial patterning of cholinergic amacrine cells in the mouse retina

Wolfram syndrome 1 (Wfs1) gene expression in the normal mouse visual system

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