Comparative Study of Exfoliated Oral Mucosal Cell Micronuclei Frequency in Normal, Precancerous and Malignant Epithelium

Halder, Arijit; Chakraborty, Tulika; Mandal, Kamalika; Gure, Prasanta Kumar; Das, Srinibas; Raychowdhury, Ranjan; Ghosh, Ashish; De, Madhusnata

doi:10.1080/09723757.2004.11885903

Cited by 36 publications

(39 citation statements)

References 12 publications

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“…These findings indicate a genotoxic damage of genotoxic agents and their metabolic products to exfoliated buccal epithelial cells. We found similar results in studies done by Parvathi et al and Halder et al [14,15] In the past, there are studies reported with significant micronuclei values in PMDs as compared to normal participants. In our study, we also found similar results.…”

Section: Discussionsupporting

confidence: 81%

Evaluation of micronuclei in betel quid chewers, potentially malignant oral disorders, and oral squamous cell carcinoma patients: A cytological assay

Somashekhar¹,

Kamath²

2016

JCRI

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Background:The micronuclei are round to oval extranuclear cytoplasmic bodies associated with chromosomal aberrations. Micronuclei are reported to be prognostic biomarkers in the screening of potentially malignant disorders (PMDs) and oral cancer. The present study was done to determine, compare, and correlate micronuclei count in normal participants, betel quid chewers, PMDs, and oral squamous cell carcinoma patients (OSCC). Materials and Methods:The buccal smears in each group were stained by Papanicolaou technique using commercially available staining kit RAPIDPAP®. Micronuclei were identified and counted in all the cases using standardized techniques. Comparative statistical evaluation was done within and among the groups. Results: The median value of micronuclei in each group was assessed using KruskalWallis test. Intergroup comparison was done using Dunn's Multiple Comparison Test. A significant increase in the median values of micronuclei in betel quid chewers was found compared to normal participants. The median values of micronuclei were significantly higher in OSCC patients followed by oral submucous fibrosis (OSF), leukoplakia, oral lichen planus, and betel quid chewers as compared to normal participants. Conclusion:The median values of micronuclei are indicative of cytogenetic damage induced by potential carcinogens. Micronuclei counts are reliable cytogenetic tools in the progression of carcinogenesis.

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Section: Discussionsupporting

confidence: 81%

Evaluation of micronuclei in betel quid chewers, potentially malignant oral disorders, and oral squamous cell carcinoma patients: A cytological assay

Somashekhar¹,

Kamath²

2016

JCRI

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“…The oral premalignant condition is defined as "a generalized state associated with a significantly increased risk of cancer." [4] Micronuclei (MN) are used as a biomarker for assessing damage to the cell for determination of population at risk. MN is studied from exfoliated buccal mucosa of smokers.…”

Section: Introductionmentioning

confidence: 99%

Micronuclei as prognostic marker: A clinicopathologic study

Sherashiya¹,

Nagaraj²,

Hemavathy³

et al. 2016

JMRPS

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Background: Tobacco products attribute to oral cancer. Chronic smoking causes permanent damage to the oral mucosal cells and results in micronuclei (MN) formation. Aims and Objectives: The present study design was to assess the MN number in different locations of the oral cavity and to assess the damaging effects in patients who smoke and those who had left the habit recently. Materials and Methods:Three groups were included in the study. The first group included smokers; the second group included those who recently quit the smoking habit; and the third group included those without habit. Cytosmears were taken and stained with rapid Papanicolaou. MN were observed using a polarized light microscope. Results: Mean values of MN were observed more in the buccal mucosa, followed by lower labial mucosa and floor of the mouth. A significant increase in MN was observed in smokers group, a significant decrease in recently quit habit group and no MN observed in the control group. Conclusion: The genotoxic effects of tobacco smoke cause MN, and the counts can be used as a non-invasive early diagnostic tool. This finding was important in using MN detection in various areas of the mouth and a prognostic indicator to determine smoking cessation.

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“…This could be achieved by detecting damage to DNA at a very early stage. Assessment of micronuclei has been a marker for genotoxic damage in assessment of nuclear changes in tobacco users as well as in development of premalignancies as shown in studies by Kayal et al 20 Suhas et al 21 Halder et al 22 Kamboj and Mahajan, 23 Sellappa et al 24 The present study showed a significant increase in number of micronuclei from healthy state to premalignancy to malignancy and reflects the findings of studies by Halder A et al 22 and Kamboj et al 23 However, in the present study there was no significant correlation between tobacco usage (frequency and duration) and the frequency of micronucleated oral mucosal cells which is contrary to the findings by Kayal et al 20 Halder et al 22 Kamboj et al 23 Gabriel et al 3 Palve et al 15 This questions the premise of cancer developing irrespective of genotoxic etiology. Presently, many cancers are seen to occur in the absence of a history of harmful habits while environmental pollution exposes people to carcinogens more frequently.…”

Section: Discussionmentioning

confidence: 99%

Micronuclei and sialic acid as markers of genotoxic damage in tobacco-related oral lesions

et al. 2013

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Background: Carcinogens and co-carcinogens act in an additive, synergistic and antagonistic manner. Tobacco is the most common carcinogen related to oral cancer. The aim of this study was to assess the effect of tobacco on the oral epithelium by evaluating genotoxic damage to cells as quantitated by micronuclei and also the alterations to the cell membrane were correlated by the measurement of free sialic acid and protein-bound sialic acid in saliva and in serum. Materials and Methods:Blood samples collected from 15 untreated oral squamous cell carcinoma patients, 15 patients with oral precancerous conditions, 15 tobacco chewers without clinically evident lesion and 15 non-tobacco chewers. Serum and salivary sialic acid levels both in free and bound form were measured spectrophotometrically. Smear was taken from the same patient from the suspicious site and assessment of micronuclei was done by acridine orange method. Conclusion:Micronuclei, serum and salivary sialic acid levels may be a good marker for prediction of premalignancy and oral squamous cell carcinoma transformation.

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Comparative Study of Exfoliated Oral Mucosal Cell Micronuclei Frequency in Normal, Precancerous and Malignant Epithelium

Cited by 36 publications

References 12 publications

Evaluation of micronuclei in betel quid chewers, potentially malignant oral disorders, and oral squamous cell carcinoma patients: A cytological assay

Evaluation of micronuclei in betel quid chewers, potentially malignant oral disorders, and oral squamous cell carcinoma patients: A cytological assay

Micronuclei as prognostic marker: A clinicopathologic study

Micronuclei and sialic acid as markers of genotoxic damage in tobacco-related oral lesions

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