Comparative study of <i>ex vivo</i> probe‐based confocal laser endomicroscopy and light microscopy in lung cancer diagnostics

Sorokina, Anastasia; Danilevskaya, Olesya; Averyanov, Alexander; Забозлаев, Ф Г; Sazonov, Dmitry; Yarmus, Lonny; Lee, Hans J.

doi:10.1111/resp.12326

Cited by 29 publications

(25 citation statements)

References 14 publications

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“…5 Specifically, several reports have mentioned that pCLE can visualize the degeneration of elastic fibers (fragmentation, disorganization, clumping, and thickening) and shows dark hollow patterns with no elastic fiber structure as a result of destruction by tumor growth. 6,7 These findings correspond to interstitial changes that occur around cancer cells. Fuchs et al stained cellular nuclei using acriflavine and reported features of normal mucosa, inflammation/regeneration, and neoplastic lesions from characteristic structures of the cytoplasm and nucleus of airway epithelial cells and tissue architecture on pCLE analysis.…”

Section: Introductionmentioning

confidence: 64%

“…In the field of bronchoscopy, Thibervill et al reported pCLE imaging of the alveolus in 2009, and various reports of this imaging technique have subsequently been published . Specifically, several reports have mentioned that pCLE can visualize the degeneration of elastic fibers (fragmentation, disorganization, clumping, and thickening) and shows dark hollow patterns with no elastic fiber structure as a result of destruction by tumor growth . These findings correspond to interstitial changes that occur around cancer cells.…”

Section: Introductionmentioning

confidence: 99%

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Probe‐based confocal laser endomicroscopy for rapid on‐site evaluation of transbronchial biopsy specimens

et al. 2019

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Background Probe‐based confocal laser endomicroscopy (pCLE) is a novel, noninvasive technology that provides real‐time lung imaging during bronchoscopy. pCLE shows the elastic fiber network without the use of a fluorescent dye. Elastic fibers produce argon laser‐induced autofluorescence at a wavelength of 488 nm, but tumor cells do not produce autofluorescence at this wavelength. As a result, the tumor cells cannot be observed directly. Therefore, we stained transbronchial biopsy (TBB) specimens with acriflavine to evaluate the benign and malignant structures using pCLE of ex vivo samples and to determine whether rapid histopathological diagnosis of TBB specimens could be made via pCLE. Methods After bronchoscopy, 36 TBB specimens were stained with acriflavine and observed using pCLE. Benign and malignant lesions were classified by cell density and nuclear magnitude disparity. Results We defined the confocal laser endomicroscopic atypia classification from the findings of the cells. The sensitivity for malignancy was 91.3%, and the specificity was 76.9%. Both inter‐observer (κ = 0.48) and intra‐observer (κ = 0.57) agreement confirmed moderate agreement. Conclusion pCLE with acriflavine staining was useful to differentiate malignant from benign TBB specimens, and might be useful as a substitute for rapid on‐site evaluation of histopathological diagnosis.

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Section: Introductionmentioning

confidence: 64%

Section: Introductionmentioning

confidence: 99%

Probe‐based confocal laser endomicroscopy for rapid on‐site evaluation of transbronchial biopsy specimens

et al. 2019

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“…Various processes disrupt this consistent elastin composition, including NSCLC. Recent work has shown that there are some specific changes seen on pCLE that correspond to specific diagnosis as seen on ex vivo tissue . The in vivo changes seen in lung cancer are the subjects of this project.…”

Section: Discussionmentioning

confidence: 99%

Comparison of in vivo probe‐based confocal laser endomicroscopy with histopathology in lung cancer: A move toward optical biopsy

et al. 2015

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“…The pCLE images showed the differences among various subtypes of lung cancer according to the stromal and parenchymal component, allowing to identify lung carcinoma in ex-vivo samples (45). On the basis of these results, Wellikoff et al described the pCLE changes for in-vivo diagnoses of lung cancer.…”

Section: Clementioning

confidence: 99%

The role of bronchoscopy in the diagnosis of early lung cancer: a review

Andolfi¹,

Potenza²,

Capozzi³

et al. 2016

J. Thorac. Dis.

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Lung cancer is the leading cause of cancer-related deaths worldwide with an overall 5-year survival rate of 17% after diagnoses. Indeed many patients tend to have a very poor prognosis, due to being diagnosed at an advanced stage. Conversely patients who are diagnosed at an early stage have a 5-year survival >70%, indicating that early detection of lung cancer is crucial to improve survival. Although flexible bronchoscopy is a relatively non-invasive procedure for patients suspected of having lung cancer, only 29% of carcinoma in situ (CIS) and 69% of microinvasive tumors were detectable using white light bronchoscopy (WLB) alone. As a result, in the past two decades, new bronchoscopic techniques have been developed to increase the yield and diagnostic accuracy, such as autofluorescence bronchoscopy (AFB), narrow band imaging (NBI) and high magnification bronchovideoscopy (HMB). However, due to the low specificity and the limitation to detect only proximal bronchial tree, new probe-based technologies have been introduced: radial endobronchial ultrasound (R-EBUS), optical coherence tomography (OCT), confocal laser endomicroscopy (CLE) and laser Raman spectroscopy (LRS). To date, although tissue biopsy remains the gold standard for diagnosing malignant/premalignant airway disease and some techniques are still investigational, bronchoscopic technologies can be considered the safest and most accurate tools to evaluate both central and distal airway mucosa.

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Comparative study of ex vivo probe‐based confocal laser endomicroscopy and light microscopy in lung cancer diagnostics

Abstract: pCLE can identify lung carcinoma in ex vivo samples. Certain light microscopy features of lung carcinoma can be visualized with pCLE.

Cited by 29 publications

References 14 publications

Probe‐based confocal laser endomicroscopy for rapid on‐site evaluation of transbronchial biopsy specimens

Probe‐based confocal laser endomicroscopy for rapid on‐site evaluation of transbronchial biopsy specimens

Comparison of in vivo probe‐based confocal laser endomicroscopy with histopathology in lung cancer: A move toward optical biopsy

The role of bronchoscopy in the diagnosis of early lung cancer: a review

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