Comparative study of natural and synthetic phenolic compounds as efficient laccase mediators for the transformation of cationic dye

Bibi, Ismat; Bhatti, Haq Nawaz; Asgher, Muhammad

doi:10.1016/j.bej.2011.07.002

Cited by 48 publications

(23 citation statements)

References 28 publications

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“…Our results concur with those of other studies of the photocatalytic degradation of Rhodamine B [46,47,48] and Triphenylmethane dyes, such as Crystal Violet [39], Malachite Green [49], and Basic Violet 3 [50], that report similar N -demethylation, desulfonation, and chromophoric ring openings during dye degradation.…”

Section: Resultssupporting

confidence: 92%

Differential Degradation and Detoxification of an Aromatic Pollutant by Two Different Peroxidases

et al. 2017

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Enzymatic degradation of organic pollutants is a new and promising remediation approach. Peroxidases are one of the most commonly used classes of enzymes to degrade organic pollutants. However, it is generally assumed that all peroxidases behave similarly and produce similar degradation products. In this study, we conducted detailed studies of the degradation of a model aromatic pollutant, Sulforhodamine B dye (SRB dye), using two peroxidases—soybean peroxidase (SBP) and chloroperoxidase (CPO). Our results show that these two related enzymes had different optimum conditions (pH, temperature, H2O2 concentration, etc.) for efficiently degrading SRB dye. High-performance liquid chromatography and liquid chromatography –mass spectrometry analyses confirmed that both SBP and CPO transformed the SRB dye into low molecular weight intermediates. While most of the intermediates produced by the two enzymes were the same, the CPO treatment produced at least one different intermediate. Furthermore, toxicological evaluation using lettuce (Lactuca sativa) seeds demonstrated that the SBP-based treatment was able to eliminate the phytotoxicity of SRB dye, but the CPO-based treatment did not. Our results show, for the first time, that while both of these related enzymes can be used to efficiently degrade organic pollutants, they have different optimum reaction conditions and may not be equally efficient in detoxification of organic pollutants.

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Section: Resultssupporting

confidence: 92%

Differential Degradation and Detoxification of an Aromatic Pollutant by Two Different Peroxidases

et al. 2017

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“…Furthermore, researchers have reported examining the efficiency of six different mediators together with fenugreek seed peroxidase (with HOBT being the best), to decolorize textile effluents (Husain et al, 2010). In another study it was demonstrated that Malachite Green (MG) decolorization was enhanced in the presence of all mediators tested, however vanillin was found to be more effective in laccasemediated degradation of MG than HOBT (Bibi et al, 2011). Additionally, this study also demonstrated that laccase/mediators system could produce metabolites that were less toxic than the parent compound (Bibi et al, 2011).…”

Section: Presence Of Redox Mediators On Dye Degradationmentioning

confidence: 95%

“…In another study it was demonstrated that Malachite Green (MG) decolorization was enhanced in the presence of all mediators tested, however vanillin was found to be more effective in laccasemediated degradation of MG than HOBT (Bibi et al, 2011). Additionally, this study also demonstrated that laccase/mediators system could produce metabolites that were less toxic than the parent compound (Bibi et al, 2011). Interestingly, a different laccase-mediated study showed that HOBT was the best mediator in decolorizing textile wastewater effluent, but it was found that only laccaseeacetosyringone treated effluent was not toxic, whereas crude and laccaseeHOBT treated effluent remained toxic (Khlifi et al,2010).…”

Section: Presence Of Redox Mediators On Dye Degradationmentioning

confidence: 95%

Fungal decolouration and degradation of azo dyes: A review

Sen¹,

Raut

Bandyopadhyay³

et al. 2016

Fungal Biology Reviews

343

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“…Some common examples of enzymes used for dye degradation are lignin peroxidase, laccases, horseradish peroxidase, tyrosinase, Manganese peroxidase etc. [14-16]. Peroxidases that have been used for treatment of aqueous aromatic contaminants and degradation of dyes include horseradish peroxidase (HRP), lignin peroxidase (LiP) and a number of other peroxidases from different sources.…”

Section: Introductionmentioning

confidence: 99%

Mechanistic study of a diazo dye degradation by Soybean Peroxidase

Kalsoom

Ashraf

Meetani

et al. 2013

Chemistry Central Journal

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BackgroundEnzyme based remediation of wastewater is emerging as a novel, efficient and environmentally-friendlier approach. However, studies showing detailed mechanisms of enzyme mediated degradation of organic pollutants are not widely published.ResultsThe present report describes a detailed study on the use of Soybean Peroxidase to efficiently degrade Trypan Blue, a diazo dye. In addition to examining various parameters that can affect the dye degradation ability of the enzyme, such as enzyme and H2O2 concentration, reaction pH and temperature, we carried out a detailed mechanistic study of Trypan Blue degradation. HPLC-DAD and LC-MS/MS studies were carried out to confirm dye degradation and analyze the intermediate metabolites and develop a detailed mechanistic dye degradation pathway.ConclusionWe report that Soybean peroxidase causes Trypan Blue degradation via symmetrical azo bond cleavage and subsequent radical-initiated ring opening of the metabolites. Interestingly, our results also show that no high molecular weight polymers were produced during the peroxidase-H2O2 mediated degradation of the phenolic Trypan Blue.

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Comparative study of natural and synthetic phenolic compounds as efficient laccase mediators for the transformation of cationic dye

Cited by 48 publications

References 28 publications

Differential Degradation and Detoxification of an Aromatic Pollutant by Two Different Peroxidases

Differential Degradation and Detoxification of an Aromatic Pollutant by Two Different Peroxidases

Fungal decolouration and degradation of azo dyes: A review

Mechanistic study of a diazo dye degradation by Soybean Peroxidase

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