Comparative study of reversed-phase high-performance liquid chromatography<i>versus</i>thin-layer chromatography—densitometry for determination of citicoline sodium in presence of its alkaline degradation products

Mahmoud, Omnia; Hegazy, Maha A.; Salem, Hesham; Moustafa, Azza A.

doi:10.1556/1006.2015.28.3.8

Cited by 3 publications

(1 citation statement)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Different analytical techniques were reported in the literature for citicoline determination in different pharmaceutical formulations. These techniques include spectrophotometry [ 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 ], spectrofluorimetry [ 12 ], densitometry [ 13 ], high performance liquid chromatography [ 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 ]. All these methods suffer from different severe limitations such as expensive instruments being required, being time consuming, non reliable results being obtained, multiple steps for sample preparation being needed and unstable reagent being used.…”

Section: Introductionmentioning

confidence: 99%

Novel Validated Analytical Method Based on Potentiometric Transduction for the Determination of Citicoline Psychostimulant/Nootropic Agent

et al. 2020

View full text Add to dashboard Cite

Herein, a novel validated potentiometric method is presented for the first time for citicoline determination. The method is based on measuring the potential using new constructed citicoline electrodes. The electrodes are based on the use of citicolinium/phosphomolybdate [Cit]2[PM] (sensor I) and citicolinium/tetraphenylborate [Cit][TPB] (sensor II) ion association complexes. These sensory materials were dispersed in plasticized polyvinyl chloride (PVC) polymeric membranes. The sensors revealed a Nernstian response with the slopes 55.9 ± 1.8(r2 = 0.9994) and 51.8 ± 0.9 (r2 = 0.9991) mV/decade over a linearity range of 6.3 × 10−6–1.0 × 10−3 and 1.0 × 10−5–1.0 × 10−3 M and detection limits of 3.16 × 10−6 and 7.1 × 10−6 M for sensors I and II, respectively. To ensure the existence of monovalent citicoline, all measurements were performed in 50 mM acetate buffer at pH 3.5. All presented electrodes showed good performance characteristics such as rapid response, good selectivity, high potential-stability and long life-span. Method verification and validation in terms of response linearity, quantification limit, accuracy, bias, trueness, robustness, within-day variability and between-days variability were evaluated. The method was introduced for citicoline determination in different pharmaceutical formulations and compared with the standard high performance liquid chromatography (HPLC) method.

show abstract

Section: Introductionmentioning

confidence: 99%

Novel Validated Analytical Method Based on Potentiometric Transduction for the Determination of Citicoline Psychostimulant/Nootropic Agent

et al. 2020

View full text Add to dashboard Cite

show abstract

Development and validation of stability indicating chromatographic methods for simultaneous determination of citicoline and piracetam

Abdelrahman

Ahmed

Omar

et al. 2020

J of Separation Science

View full text Add to dashboard Cite

Citicoline and piracetam were subjected separately to different stress conditions as recommended by the international conference on harmonization. In addition, new stability indicating thin layer chromatographic and ultra high performance liquid chromatographic methods have been developed and validated for simultaneous determination of citicoline and piracetam in presence of their degradation products. Separation on the proposed thin layer chromatographic method was carried out using a developing system containing methanol:chloroform:ammonium chloride buffer (9:1:2, v/v/v) on silica gel plates at 230 nm. On the other hand, the mobile phase in the ultra high performance liquid chromatographic method was composed of water (containing 0.1% triethylamine):ethanol (92:8, v/v). The flow rate was 1 mL/min and ultraviolet detection was at 230 nm. Moreover, results of the developed methods were statistically compared to those obtained by the reported high‐performance liquid chromatography method and no significant difference between them was found. The greenness profile of ultra high performance liquid chromatographic method was assessed and compared with those of the previously published high‐performance liquid chromatography methods, it was noticed that the proposed ultra high performance liquid chromatographic method more environmentally friendly and greener than other methods.

show abstract

Spectrofluorimetric approach for determination of citicoline in the presence of co-formulated piracetam through fluorescence quenching of eosin Y

Omar

Ahmed

Abdelwahab

et al. 2020

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

View full text Add to dashboard Cite

Comparative study of reversed-phase high-performance liquid chromatographyversusthin-layer chromatography—densitometry for determination of citicoline sodium in presence of its alkaline degradation products

Cited by 3 publications

References 6 publications

Novel Validated Analytical Method Based on Potentiometric Transduction for the Determination of Citicoline Psychostimulant/Nootropic Agent

Novel Validated Analytical Method Based on Potentiometric Transduction for the Determination of Citicoline Psychostimulant/Nootropic Agent

Development and validation of stability indicating chromatographic methods for simultaneous determination of citicoline and piracetam

Spectrofluorimetric approach for determination of citicoline in the presence of co-formulated piracetam through fluorescence quenching of eosin Y

Contact Info

Product

Resources

About