COMPARATIVE STUDY OF Sida rhombifolia FROM TWO DIFFERENT LOCATIONS

Abstract

Sida rhombifolia or sidaguri, was taken from two regions in East Java Province, Indonesia, namely Batu (SB) and Ngawi (SN), was investigated in this study. The two samples were determined the content of secondary metabolites by using LC-MS/MS. They were also evaluated for their phytochemical content, total phenolic content (TPC), and antioxidants activity, using the DPPH and ABTS methods. The highest TPC (126.93 ± 0.63 mg GAE / g) was observed in the ethyl acetate fraction of SN. The antioxidant activity against DPPH and ABTS from crude extracts and fractions showed that SB was more potent than SN, although the values were both less active. A total of 54 secondary metabolites were identified by the LC-MS-MS method from both species, with SB samples producing more secondary metabolites (48 compounds) than SN (37 compounds). These compounds are included in lipids, alkaloids, ester lactones, benzoic acid derivatives, steroids, carboxylic acid derivatives, flavonoids, and phenyl propanoic.

FT-IR Fingerprinting Analysis for Classification of West Sumatra Small Ginger (Zingiber officinale Roscoe) Essential Oil and Its Antioxidant Activity

“…33 The previous study concluded that the antioxidant activity may vary due to various factors such as the method of testing employed and the location from which the plant was collected. 34,35…”

UHPLC‐Q‐Orbitrap HR‐MS‐Based Metabolomics for Profiling the Sida rhombifolia Metabolites with Different Plant Organs and Cultivation Ages

“…Eleven metabolites derived from phenolic acid and phenolic groups aldehydes were identified in the S. rhombifolia leaves, stems, and roots methanol extract. We successfully identified six phenolic acid metabolites, that is, caffeic acid (1), chlorogenic acid (2), ferulic acid (3), p-coumaric acid (4), salicylic acid (5), and vanillic acid (6), as well as four phenolic aldehyde compounds namely coniferaldehyde (7), ethoxyferulate (8), evofolin-A (9), sinapaldehyde (10), and syringaldehyde (11). The typical fragmentation pattern of phenolic acid metabolites releases the COOH, H2O, and RCOOH functional groups.…”

“…It is also known that secondary metabolites in plants are most commonly found on leaves because they are more easily affected by changes in environmental conditions [5] . For example, previous research has shown that sidaguri grown at different locations has different metabolite profiles [6] . Therefore, it is necessary to identify metabolites in S. rhombifolia leaves, stems, and roots at different cultivation ages as preliminary information about metabolite composition and concentration.…”

“…[5] For example, previous research has shown that sidaguri grown at different locations has different metabolite profiles. [6] Therefore, it is necessary to identify metabolites in S. rhombifolia leaves, stems, and roots at different cultivation ages as preliminary information about metabolite composition and concentration.…”