Comparative study of the action of flunixin meglumine and tolfenamic acid on prostaglandin E₂ synthesis in bovine inflammatory exudate

Abstract

An acute non-immune inflammation model was used to compare the action of two non-steroidal anti-inflammatory drugs, flunixin meglumine and tolfenamic acid, on prostaglandin E2 (PGE2) synthesis in bovine inflammatory exudate. The tissue cage model used involves subcutaneous implantation of polypropylene cages and subsequent stimulation by carrageenan injection of the granulation tissue which develops within the cage. Twelve calves were randomly assigned to three groups receiving placebo, flunixin meglumine and tolfenamic acid, respectively. Inflammatory exudate was sampled 30 min after carrageenan injection and at seven subsequent time points. PGE2 levels were determined by radioimmunoassay. At each time point post-carrageenan injection, flunixin meglumine inhibited PGE2 synthesis to a greater extent than tolfenamic acid. At 4, 8, 12 and 24 h these differences were statistically significant.

Effects of steroidal and non-steroidal antiphlogistic drugs on eicosanoid synthesis in irritated skin: studies with the isolated perfused bovine udder

“…As expected, flunixin meglumine had no influence on the lipoxygenase pathway. This confirmed the in-vivo results of Espinasse et al (1994).…”

“…After 1 h, a slightly enhanced PGE 2 concentration was obvious. Compared with the in-vivo results of Espinasse et al (1994), where the inhibition set in 30 min after administration of flunixin, our model took a longer time. The reason for this is because in contrast to the in-vivo experiments where the eicosanoids were measured subcutaneously, in our in-vitro model flunixin had to diffuse into the epidermis and dermis as the loci of inflammation.…”

The Isolated Perfused Bovine Udder as a Model of Dermal Eicosanoid Release

“…This is obviously due to biological variability. Espinasse et al (15) showed a comparable variability in bovine exudate in vivo. In addition, the variability of the PGE 2 concentration may also have been caused by the slightly differing depth of the microdialysis membrane.…”

Flunixin meglumine accelerates uterine involution and shortens the calving‐to‐first‐oestrus interval in cows with puerperal metritis

“…These findings are consistent with the antipyretic, anti‐inflammatory and antitoxic properties of flunixin. In cattle, flunixin reduces the plasma concentration of thromboxane B 2 and prostaglandin E 2 and thus, is effective against inflammatory response to endotoxins (Anderson & Hunt, 1989; Espinasse et al ., 1994; Odensvik & Magnusson, 1996).…”