Comparative study of the cell tropism of feline immunodeficiency virus isolates of subtypes A, B and D classified on the basis of the env gene V3-V5 sequence

Hohdatsu, Tsutomu; Hirabayashi, Hiroyuki; Motokawa, Kenji; Koyama, Hiroyuki

doi:10.1099/0022-1317-77-1-93

Cited by 43 publications

(38 citation statements)

References 33 publications

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“…Treatment of chronically infected cells with vpg15 reduced the numbers of budding particles at the plasma membrane and inhibited FIV spread in cell culture, so it appears likely that viral assembly and/or release (29) is inhibited by anti-CD9 antibody, indicating a role for fCD9 similar to that of other tetraspanins in viral infections. FIV can infect CD9-negative 3201 cells (55), implying that CD9 is not essential for FIV infection or at least that other tetraspanins can take over this role. However, ectopic expression of CD9 in 3201 cells enhances FIV infectivity, suggesting that CD9 plays a direct role in infection rather than an indirect role via cell signaling pathways affected by anti-CD9 antibody ligation (163).…”

Section: Htlv-1 Htlv-1 Is a Type C Retrovirus That Infects Cd4mentioning

confidence: 99%

Tetraspanins in Viral Infections: a Fundamental Role in Viral Biology?

Martin

Roth

Jans

et al. 2005

J Virol

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4The tetraspanins are a broadly expressed superfamily of transmembrane glycoproteins with over 30 members found in humans and with homologues conserved through distantly related species, including insects, sponges, and fungi. Members of this family appear to form large integrated signaling complexes or tetraspanin-enriched microdomains (TEMs) by their association with a variety of transmembrane and intracellular signaling/cytoskeletal proteins (49). These interactions link tetraspanins to an array of physiological functions and, in consequence, to numerous endogenous pathologies, including cancer development and inherited disorders (Table 1).Tetraspanins are also known to have roles in the pathology of infectious diseases such as diphtheria, malaria, and numerous viral infections (Table 1). The literature currently indicates that specific tetraspanin family members are selectively associated with specific viruses and affect multiple stages of infectivity, from initial cellular attachment to syncytium formation and viral particle release. Thus, the relationship of tetraspanins with viruses appears to be particularly complex.Here, we will consider this data in the context of recent developments in tetraspanin biology, particularly in our understanding of the architecture and function of TEMs. With the benefit of recent insights into tetraspanin function in cell fusion events and intracellular trafficking, we discuss common features of tetraspanin/viral associations which indicate a fundamental role for TEMs in a number of viral infections. We will also consider the existing therapeutic strategies for human immunodeficiency virus (HIV), hepatitis C virus (HCV), and human T-cell lymphotropic virus type 1 (HTLV-1), focusing on the potential therapeutic value of targeting TEMs, using peptide reagents based on tetraspanin extracellular regions.

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Section: Htlv-1 Htlv-1 Is a Type C Retrovirus That Infects Cd4mentioning

confidence: 99%

Tetraspanins in Viral Infections: a Fundamental Role in Viral Biology?

Martin

Roth

Jans

et al. 2005

J Virol

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“…The feline T-lymphocyte FeT-J cells (CD4+, CD8±) used in this study were obtained from the American Type Culture Collection (ATCC CRL11967) [9,29]. Cultures were incubated at 37°C in a humidified atmosphere of 5% CO 2 -in-air and routinely subcultured every 4 days in RPMI 1640 growth medium (Invitrogen, Carlsbad, CA, U.S.A.) supplemented with 10% heatinactivated fetal bovine serum (Equitech-Bio, Kerrville, TX, U.S.A.) and 0.1 µg/ml kanamycin (Invitrogen).…”

Section: Cell Cultures and Irradiationmentioning

confidence: 99%

Killing of Feline T-Lymphocytes by Gamma-Rays and Energetic Carbon Ions

Kakizaki

Hamada

Funayama

et al. 2006

The Journal of Veterinary Medical Science

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ABSTRACT. High linear energy transfer (LET) heavy charged particles have previously been applied clinically to human cancer radiotherapy because of their excellent physical properties of selective dose distribution and higher relative biological effectiveness (RBE) for human; however, such an approach has yet to be applied to cat patients. The present study investigates the biological effectiveness of low-LET γ-rays (0.2 keV/µm) compared to high-LET carbon ions (114 keV/µm) in feline T-lymphocyte FeT-J cells. Clonogenic survival analysis revealed that the RBE value of carbon ions was 2.98 relative to a 10% survival dose (D 10 ) by γ-rays, and that the inactivation cross-section in cells exposed to γ-rays and carbon ions was 0.023 and 38.9 µm 2 , respectively. Terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick end labeling (TUNEL) analysis revealed that TUNEL-positive frequency in carbon-irradiation cells is higher than for γ-irradiated cells against exposure to the same physical doses, but that very little difference in TUNEL-positive frequency is observed between cells exposed to the respective D 10 dose of γ-rays. Our data thus indicate that carbon ions are more effective for cell killing than γ-rays at the same physical doses, but kill cells to an extent that is comparable to γ-rays at the same biological doses. Carbon ion radiotherapy is therefore a promising modality for cat patients. KEY WORDS: accelerated carbon ions, apoptosis, feline T lymphocytes, gamma-ray, relative biological effect.

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“…FIV isolates from domestic cats have been detected all over the world. Five pure subtypes of FIV (A, B, C, D, and E) and their mosaic forms have been identified, based on analysis of V3-V5 env gene and p25 gag gene (12,28,33,39). Subtypes A and B are the most common worldwide isolates.…”

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confidence: 99%

Phylogenetic and Genetic Analysis of Feline Immunodeficiency Virus gag , pol , and env Genes from Domestic Cats Undergoing Nucleoside Reverse Transcriptase Inhibitor Treatment or Treatment-Naïve Cats in Rio de Janeiro, Brazil

Martins

Medeiros

Simonetti

et al. 2008

J Virol

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Feline immunodeficiency virus (FIV) is the Lentivirus responsible for an immunodeficiency-like disease in domestic cats (Felis catus). FIV is divided into five phylogenetic subtypes (A, B, C, D, and E), based on genetic diversity. Knowledge of the geographical distribution of subtypes is relevant for understanding different disease progressions and for vaccine development. In this study, viral sequences of 26 infected cats from Rio de Janeiro, 8 undergoing treatment with zidovudine (AZT) for at least 5 years, were successfully amplified from blood specimens. gag capsid (CA), pol reverse transcriptase (RT), and env gp120 (V3-V4) regions were analyzed to determine subtypes and to evaluate potential mutations related to antiretroviral drug resistance among treated cats. Subtyping based on phylogenetic analysis was performed by the neighbor-joining and maximum likelihood methods. All of the sequences clustered with subtype B in the three regions, exhibiting low genetic variability. Additionally, we found evidence that the same virus is circulating in animals in close contact. The analysis of FIV RT sequences identified two new putative mutations related to drug resistance located in the RT "finger" domain, which has 60% identity to human immunodeficiency virus (HIV) sequence. Amino acid change K3R at codons 64 and 69 was found in 25% and 37.5% of the treated animals, respectively. These signatures were comparable to K65R and K70R thymidine-associated mutations found in the HIV-1 HXB2 counterpart. This finding strongly suggests a position correlation between the mutations found in FIV and the K65R and K70R substitutions from drug-resistant HIV-1 strains.

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Comparative study of the cell tropism of feline immunodeficiency virus isolates of subtypes A, B and D classified on the basis of the env gene V3-V5 sequence

Cited by 43 publications

References 33 publications

Tetraspanins in Viral Infections: a Fundamental Role in Viral Biology?

Tetraspanins in Viral Infections: a Fundamental Role in Viral Biology?

Killing of Feline T-Lymphocytes by Gamma-Rays and Energetic Carbon Ions

Phylogenetic and Genetic Analysis of Feline Immunodeficiency Virus gag , pol , and env Genes from Domestic Cats Undergoing Nucleoside Reverse Transcriptase Inhibitor Treatment or Treatment-Naïve Cats in Rio de Janeiro, Brazil

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