Comparative study of toxicity and biochemical responses induced by sublethal levels of the pesticide azinphosmethyl in two fish species from North-Patagonia, Argentina

Guerreño, Mariana; Armengol, María Fernanda López; Luquet, Carlos Marcelo; Venturino, Andrés

doi:10.1016/j.aquatox.2016.06.015

Cited by 18 publications

(2 citation statements)

References 28 publications

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“…Various kinds of pesticides have been detected in super cial waters where amphibian reproduction occurs 2 . This situation implies that both R. arenarum embryos and larvae are potentially exposed, at least temporarily, to high concentrations of these toxicants, posing a hazard to this and other species that inhabit the area 3,4,5,6,7,8,9,10 .…”

Section: Introductionmentioning

confidence: 99%

mRNA Transcript Analysis of Hypothesis-driven Pathways as Known Responders to Organophosphate Exposure: Rhinella Arenarum Larvae Transcriptome Study

Pires

Lascano

Ceschin³

et al. 2022

Preprint

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Transcriptional analysis of the network of transcription regulators and target pathways in exposed organisms may be a hard task when their genome remains unknown. We used a whole transcriptome study on Rhinella arenarum larvae exposed to the organophosphorus pesticides azinphos-methyl and chlorpyrifos to evaluate transcriptional effects on a priori selected groups of genes. This approach allowed us to evaluate the effects on hypothesis-selected pathways such as target esterases, detoxifying enzymes, polyamine metabolism and signaling and regulatory pathways modulating them. We could then compare the responses at the transcriptional level with previously described effects at the enzymatic or metabolic levels to obtain global insight into toxicity-response mechanisms. The effects of both pesticides on the transcript levels of these pathways could be considered moderate, while the responses elicited by chlorpyrifos were more potent and earlier than those elicited by azinphos-methyl. Finally, we infer a prevailing downregulation effect of pesticides on signaling pathways and transcription factor transcripts encoding products that modulate/control the polyamine and antioxidant response pathways. We additionally tested and selected potential housekeeping genes based on those reported for other species. These results allow us to go through future confirmatory studies on pesticide gene expression modulation in toad larvae.

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Section: Introductionmentioning

confidence: 99%

mRNA Transcript Analysis of Hypothesis-driven Pathways as Known Responders to Organophosphate Exposure: Rhinella Arenarum Larvae Transcriptome Study

Pires

Lascano

Ceschin³

et al. 2022

Preprint

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“…In turn, biochemical biomarkers may in general anticipate to these probably irreversible effects and should be considered as more effective endpoints to set the permitted environmental limits (Figure 5). Moreover, the study of a battery of biochemical biomarkers may particularly reveal some aspects of the toxic mechanisms on different organisms differing in their responses to any particular toxicant (Guerreño et al, 2016).…”

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confidence: 99%

Toxicogenomics: new strategies for ecotoxicology studies in autochthonous species I. A glade in the entangled path towards the 'fingerprint' of environmental impact?

Venturino

2017

IJENVH

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The search of early and sensitive biomarkers pointing to toxic effects of contaminants over organisms, has led the state of art to the molecular mechanisms of signalling, target and response. The trial to obtain more specific biomarkers of response to toxicants was in general biased by the finding of multiple crosstalks between signalling and the transcription factor regulation on gene expression. This may result in virtually impossible application in field ecotoxicological studies and biomonitoring, when native or local species must be used. Molecular biomarkers of toxicity generally show higher sensitivity and precocity in the response, while classical biomarkers such as cholinesterases in organisms exposed to anticholinesterasic agents may show "inconsistent" responses. The use of non-model aquatic organisms in general defies the application of commercially available antibodies and implies the design of primers for gene expression studies from model species sequences. The application of transcriptomics has opened a new way to gene annotation and for the design of adequate molecules as tools for the research in new biomarkers in autochthonous species.

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Land-Use Effects on Aquatic and Wetland Ecosystems: An Overview of Environmental Impacts and Tools for Ecological Assessment

Miserendino

Brand

Assef

et al. 2022

Natural and Social Sciences of Patagonia

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Comparative study of toxicity and biochemical responses induced by sublethal levels of the pesticide azinphosmethyl in two fish species from North-Patagonia, Argentina

Cited by 18 publications

References 28 publications

mRNA Transcript Analysis of Hypothesis-driven Pathways as Known Responders to Organophosphate Exposure: Rhinella Arenarum Larvae Transcriptome Study

mRNA Transcript Analysis of Hypothesis-driven Pathways as Known Responders to Organophosphate Exposure: Rhinella Arenarum Larvae Transcriptome Study

Toxicogenomics: new strategies for ecotoxicology studies in autochthonous species I. A glade in the entangled path towards the 'fingerprint' of environmental impact?

Land-Use Effects on Aquatic and Wetland Ecosystems: An Overview of Environmental Impacts and Tools for Ecological Assessment

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