Comparative study of zinc oxide nanoparticles and its bulk form on liver function of Wistar rat

Abstract

Zinc oxide nanoparticles (ZnO NPs) are produced in high tonnage each year; they are widely used in consumer and industrial products, also now finding applications in bioimaging and drug delivery. In the present study, comparison between ZnO NPs (39 nm) and its bulk/micron form (particle size = 5 µm) on liver function of rats was determined. In our study, liver enzymes biomarkers, serum lipid profile, zinc concentration, and histopathological examination in liver tissues were used to evaluate liver injury. Moreover, lipid peroxidation (malondialdehyde), nitric oxide, and reduced glutathione levels were determined to detect the oxidation–reduction process in liver tissue. The results showed dose-dependent toxicity of ZnO NPs. Three different dose levels (25, 50, and 100 mg/kg bw) were used, and the 100-mg/kg bw ZnO NPs group showed the most significant changes in liver enzymes and histopathological structure, as well as redox state. The dose of 100 mg/kg bw of ZnO bulk group showed no significant effects on liver function. The study concluded that ZnO NPs caused hepatic impairments.

Postnatal distribution of ZnO nanoparticles to the breast milk through oral route and their risk assessment for breastfed rat offsprings

“…Further, the AST level in 50 mg kg À1 of bZnO dams increased significantly than control, but it was lower than 25 mg kg À1 of ZnO-NPs-treated group. This is consistent with the findings of Moatamed et al 56 These data reflected that the ZnO-NPs caused a breakdown of the cell membrane of hepatocytes leading to the release of these enzymes into the bloodstream. Thus, the increase in these enzymes is a traditional marker for liver injury.…”

“…40 Furthermore, oxidative stress and ROS production are referred to as the most discussed reason for the toxicity of NPs. 56,[60][61][62] The increase in anti-inflammatory cytokines from the plasma of Sprague-Dawley rats was also reported after damage to the liver on intranasal administration of ZnO-NPs. 63 Our findings also suggest that histopathological changes in the liver as a result of oxidative stress lead to alteration in various biochemical parameters.…”

Nanoencapsulation of thyme essential oil: a new avenue to enhance its protective role against oxidative stress and cytotoxicity of zinc oxide nanoparticles in rats

“…The histological examination of the hepatic and renal sections in rats received ZnO-NPs showed severe histological changes typical to those reported in the previous studies (Moatamed et al, 2019;Shaban et al, 2019). These ndings con rmed the biochemical results reported herein and supported the hypothesis that ZnO-NPs induced severe hepato-nephrotoxicity mainly through the generation of oxidative damage and the immunode ciency response.…”

Biosynthesis of ZnO Nanoparticles Using Bacillus Subtilis: Characterization and Nutritive Significance for Promoting Plant Growth in Zea mays L

“…Use of nanoparticles is becoming a promising strategy to enhance plant growth and productivity due to the presence of exceptional properties such as small size, high surface area/volume ratio, high adsorption, large number of reactive sites, high catalytic activity and high chemical stability as compared to bulk ions. 3 These properties make the nanoparticles highly reactive upon their exposure to biological systems. Many researchers focused on the bioenvironmental impact of nanoparticles particularly their effects on animals, plants and microbes.…”