BACKGROUNDPomegranate peel is a by‐product from pomegranate processing industries, rich source of dietary fibers and bioactive compounds. It has good antioxidant and antimicrobial properties. In the present investigation the effect of substitution of refined wheat flour with pomegranate peel powder PPP at the rate of 2, 4, 6, 8, and 10% on physico‐chemical, and sensorial properties as well as on oxidative and microbial stability of muffins were studied.RESULTSA significant reduction in specific volume (1.99 to 1.57 cm3 g‐1), weight loss (11.73 to 10.14g 100g‐1) and an increase in crumb hardness (633.06 to 2311.5 g) of muffins were observed on addition of PPP. Moreover, the nutritional value was improved by a significant increase in the fiber content (4.39 to 10.66 %), total phenols (0.443 to 48.53 mg GAE 100g‐1), antioxidant activity (75.94 to 99.36 %), calcium (200.33 to 294.33 mg 100g‐1), potassium (227.33 to 425.33 mg 100g‐1) and magnesium (96.33 to 288.33 mg 100g‐1). The pasting and rheological properties of muffin batter showed a significant decrease in the final and peak viscosity; and increase in storage, loss and, complex modulus. The muffin samples were organoleptically acceptable up to the level of 8% PPP. The free fatty acid content, peroxide value, and microbial count of the muffin with 8% PPP was significantly lower than the control sample and more oxidative and microbial stable for a storage period of 21 and 28 days at ambient and refrigerated temperatures respectively.CONCLUSIONSThis study provides the opportunity to use PPP as functional ingredients and natural preservative in preparation of muffins.This article is protected by copyright. All rights reserved.