Comparative study on elemental composition and DNA damage in leaves of a weedy plant species, Cassia occidentalis, growing wild on weathered fly ash and soil

Tandon, Rajesh; Banerjee, B. D.; Babu, C. R.

doi:10.1007/s10646-009-0322-5

Cited by 13 publications

(2 citation statements)

References 47 publications

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“…Their complex constitution raised questions on their genotoxicity to animals and plants. Love et al ( 2009 ) demonstrated, based upon comet assay results, that higher levels of DNA damages were found in leaves of Cassia occidentalis exposed to fly ash, compared to non-exposed controls. The authors suggested that these DNA damages might be associated with foliar concentrations of As and Ni absorbed from the fly ash.…”

Section: Contaminated Matricesmentioning

confidence: 99%

The use of comet assay in plant toxicology: recent advances

2015

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The systematic study of genotoxicity in plants induced by contaminants and other stress agents has been hindered to date by the lack of reliable and robust biomarkers. The comet assay is a versatile and sensitive method for the evaluation of DNA damages and DNA repair capacity at single-cell level. Due to its simplicity and sensitivity, and the small number of cells required to obtain robust results, the use of plant comet assay has drastically increased in the last decade. For years its use was restricted to a few model species, e.g., Allium cepa, Nicotiana tabacum, Vicia faba, or Arabidopsis thaliana but this number largely increased in the last years. Plant comet assay has been used to study the genotoxic impact of radiation, chemicals including pesticides, phytocompounds, heavy metals, nanoparticles or contaminated complex matrices. Here we will review the most recent data on the use of this technique as a standard approach for studying the genotoxic effects of different stress conditions on plants. Also, we will discuss the integration of information provided by the comet assay with other DNA-damage indicators, and with cellular responses including oxidative stress, cell division or cell death. Finally, we will focus on putative relations between transcripts related with DNA damage pathways, DNA replication and repair, oxidative stress and cell cycle progression that have been identified in plant cells with comet assays demonstrating DNA damage.

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Section: Contaminated Matricesmentioning

confidence: 99%

The use of comet assay in plant toxicology: recent advances

2015

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“…In a comparative study of DNA damage in leaves of Cassia occidentalis grown on weathered fly ash or soil, higher levels of DNA damage was detected by comet assay in leaf tissues of Cassia occidentalis growing on fly ash than C. occidentalis grown on soil (Love et al 2009). Sriussadaporn et al (2003) conducted a study to compare the level of DNA damage in different plant species grown in roadside versus non-roadside environments using comet assay.…”

Section: Introductionmentioning

confidence: 99%

Plants genotoxicity as pollution bioindicator in Jordan using comet assay

Khateeb

2017

Physiol Mol Biol Plants

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This study aimed to assess genotoxicity in wild plants grown in Jordan as a pollution bioindicator. Comet assay was used to evaluate the level of DNA damage in plants collected from different areas in Jordan. Significant differences in plant DNA damage index and frequency were observed among sites of collection. Results show that plants collected from Aqaba back road and Ghour Assaal had significantly higher damage values. In contrast, plants collected from Wadi Rum, Al Naqab Heights, Swaimeh/Deadsea and Alshoneh Aljanobyeh showed low levels of DNA damage. A similar trend was observed for lipid peroxidation rates. Furthermore, heavy metal analysis showed that plants collected from Aqaba back road and Aqaba airport had the highest Al, Cr, Fe, Cu, Zn, Cd and Pb contents. A significant correlation was observed between DNA Damage Index, DNA Damage Frequency, lipid peroxidation rate, soil Cu, Cd and Pb biomarkers, indicating that heavy metals pollution is a major source for genotoxicity in these plant species. Finally, our results approved the feasibility of using plants and Comet assay system as a diagnostic tool for pollution in any environment adversely affected by different pollution sources.

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Assessment of oxidative stress markers and concentrations of selected elements in the leaves of Cassia occidentalis growing wild on a coal fly ash basin

Banerjee

Babu

2013

Environ Monit Assess

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Assessment of oxidative stress levels and tissue concentrations of elements in plants growing wild on fly ash basins is critical for realistic hazard identification of fly ash disposal areas. Hitherto, levels of oxidative stress markers in plants growing wild on fly ash basins have not been adequately investigated. We report here concentrations of selected metal and metalloid elements and levels of oxidative stress markers in leaves of Cassia occidentalis growing wild on a fly ash basin (Badarpur Thermal Power Station site) and a reference site (Garhi Mandu Van site). Plants growing on the fly ash basin had significantly high foliar concentration of As, Ni, Pb and Se and low foliar concentration of Mn and Fe compared to the plants growing on the reference site. The plants inhabiting the fly ash basin showed signs of oxidative stress and had elevated levels of lipid peroxidation, electrolyte leakage from cells and low levels of chlorophyll a and total carotenoids compared to plants growing at the reference site. The levels of both protein thiols and nonprotein thiols were elevated in plants growing on the fly ash basin compared to plants growing on the reference site. However, no differences were observed in the levels of cysteine, reduced glutathione and oxidized glutathione in plants growing at both the sites. Our study suggests that: (1) fly ash triggers oxidative stress responses in plants growing wild on fly ash basin, and (2) elevated levels of protein thiols and nonprotein thiols may have a role in protecting the plants from environmental stress.

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Comparative study on elemental composition and DNA damage in leaves of a weedy plant species, Cassia occidentalis, growing wild on weathered fly ash and soil

Cited by 13 publications

References 47 publications

The use of comet assay in plant toxicology: recent advances

The use of comet assay in plant toxicology: recent advances

Plants genotoxicity as pollution bioindicator in Jordan using comet assay

Assessment of oxidative stress markers and concentrations of selected elements in the leaves of Cassia occidentalis growing wild on a coal fly ash basin

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