Comparative study on eri silk and mulberry silk fibroin scaffolds for biomedical applications

Muthumanickkam, A.; Sundaramoorthy, Subramanian; Goweri, Mahizhappan; Beaula, Winfred Sofi; Venkataraman, Ganesh

doi:10.1007/s13726-012-0113-3

Cited by 28 publications

(18 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The degummed fibers exhibited peaks centered at 1654 cm À1 , 1535 cm À1 , and 1240 cm À1 attributed to the typical dominant beta sheet along with turn and random coil conformation (Fig. 1C-I) [37][38][39][40][41]. When comparing the degummed fiber (Fig.…”

Section: Resultsmentioning

confidence: 94%

Fabrication and characterization of Eri silk fibers-based sponges for biomedical application

Silva

Oliveira

et al. 2016

Acta Biomaterialia

View full text Add to dashboard Cite

This work addresses the preparation and characterization of non-mulberry cocoon-derived Eri silk sponges. The insolubility of cocoons-derived non-mulberry silkworms impairs their processability and applications in the healthcare field. We used a green approach with ionic liquids to overcome the lack solubility of such silk fibers. The formation of beta-sheet structures into Eri-based sponges was physically and chemically induced. The sponges were obtained by freeze-drying. The developed structures exhibited flexibility to adapt and recover their shapes upon application and subsequent removal of load, high swelling degree, ability to load an anti-inflammatory drug and to promote its sustained release. They promoted in vitro cellular adhesion, proliferation and extracellular matrix production of a chondrocyte-like cell line, opening up their potential application for biomedical applications.

show abstract

Section: Resultsmentioning

confidence: 94%

Fabrication and characterization of Eri silk fibers-based sponges for biomedical application

Silva

Oliveira

et al. 2016

Acta Biomaterialia

View full text Add to dashboard Cite

show abstract

“…Hemolytic assay [ 29 ] was performed using the heparinized 1 ml whole human blood collected from a donor (IEC-NI/11/DEC/26/81) and the RBC pellet was obtained by centrifuging at 1000 g for 5 min. Supernatant was discarded and the RBC pellet was washed with PBS.…”

Section: Methodsmentioning

confidence: 99%

Bioactivity Studies of β-Lactam Derived Polycyclic Fused Pyrroli-Dine/Pyrrolizidine Derivatives in Dentistry: In Vitro, In Vivo and In Silico Studies

et al. 2015

View full text Add to dashboard Cite

The antibacterial activity of β-lactam derived polycyclic fused pyrrolidine/pyrrolizidine derivatives synthesized by 1, 3-dipolar cycloaddition reaction was evaluated against microbes involved in dental infection. Fifteen compounds were screened; among them compound 3 showed efficient antibacterial activity in an ex vivo dentinal tubule model and in vivo mice infectious model. In silico docking studies showed greater affinity to penicillin binding protein. Cell damage was observed under Scanning Electron Microscopy (SEM) which was further proved by Confocal Laser Scanning Microscope (CLSM) and quantified using Flow Cytometry by PI up-take. Compound 3 treated E. faecalis showed ROS generation and loss of membrane integrity was quantified by flow cytometry. Compound 3 was also found to be active against resistant E. faecalis strains isolated from failed root canal treatment cases. Further, compound 3 was found to be hemocompatible, not cytotoxic to normal mammalian NIH 3T3 cells and non mutagenic. It was concluded that β-lactam compound 3 exhibited promising antibacterial activity against E. faecalis involved in root canal infections and the mechanism of action was deciphered. The results of this research can be further implicated in the development of potent antibacterial medicaments with applications in dentistry.

show abstract

“…The hydrophilic to hydrophobic amino acid ratio for non-mulberry silks (9.06–9.85) is higher compared to that of the mulberry varieties (5.29–6.22), which results in higher moisture content of non-mulberry silks (Altman et al 2003 ; Sen and Murugesh Babu 2003 ). Silk fibroin has RGD (arginine-glycine-aspartic acid) sequence, which enhances cell adhesion, cell proliferation and differentiation (Muthumanickkam et al 2013b ; Bray et al 2013 ). Silk I is the helical protein present in the silk glands prior to spinning.…”

Section: Introductionmentioning

confidence: 99%

“…The higher moisture regain of non-mulberry silks suggests that all three non-mulberry silk varieties may consist of a higher ratio of hydrophilic to hydrophobic amino acid residues in their chemical architecture. Studies on application of mulberry silk for biomedical applications and comparison of eri and mulberry silk have been carried out by Muthumanickkam et al ( 2013b ). In the present work, a comparison was made between Eri silk ( Samiacynthia ricini ) and Tasar silk ( A. mylitta ) fibroin scaffolds.…”

Section: Introductionmentioning

confidence: 99%

Comparison of eri and tasar silk fibroin scaffolds for biomedical applications

et al. 2016

View full text Add to dashboard Cite

The cultivated silk, mulberry, is being used as biomaterial in different forms. Eri, tasar and muga are some of the known wild silk varieties. The studies on biomedical applications of electrospun mats produced from these wild silks are limited though few studies on eri silk are available. In this work, comparison was made between eri and tasar silk fibroin scaffolds for biomedical application. The scaffolds were produced from eri silk fibroin (ESF) and tasar silk fibroin (TSF) by electrospinning method and they were treated with ethanol to improve dimensional stability. Ethanol treatment increased the crystallinity% of both ESF and TSF scaffolds. The crystallinity percentage of the ESF and TSF scaffolds was found to be 46.7 and 42.8 % respectively. Thermal stability was higher for ESF than that of TSF scaffold. The hemolytic % of ESF and TSF scaffolds was found to be 1.3 and 7.7 % respectively. The platelet adhesion on the surface of ESF scaffold was lower than that found on TSF scaffold. Better fibroblast cell attachment, binding and spreading was found on the ESF scaffold. The cell viability on ESF scaffold was 83.78 % and in TSF was 78.01 % for 48 h. The results showed that ESF electrospun scaffold can be considered as a better biomaterial for biomedical applications compared to that of TSF scaffold.

show abstract

Comparative study on eri silk and mulberry silk fibroin scaffolds for biomedical applications

Cited by 28 publications

References 26 publications

Fabrication and characterization of Eri silk fibers-based sponges for biomedical application

Fabrication and characterization of Eri silk fibers-based sponges for biomedical application

Bioactivity Studies of β-Lactam Derived Polycyclic Fused Pyrroli-Dine/Pyrrolizidine Derivatives in Dentistry: In Vitro, In Vivo and In Silico Studies

Comparison of eri and tasar silk fibroin scaffolds for biomedical applications

Contact Info

Product

Resources

About