Biological tissues are a combination of cellular and acellular components. The extracellular matrix (ECM), or the acellular component of tissues, is often overlooked.Tracking changes in the ECM grants insight into how aging, diseases and treatments might affect tissue structures and cellular responses. Using gray-level texture analysis, we analyzed the architecture of fibrillar collagen, a major component of the ECM.Several gray-level textural features were extracted from second harmonic generation (SHG) images and used to characterize collagen network arrangements. This work provides a basis for a classification model aimed to track changes in the cellular microenvironment. This information is vital if we wish to continue exploration of how diseases develop in different tissues, and develop novel and effective treatments for different types of chronic diseases.Foremost, and most certainly, I would like to extend my deepest and sincere gratitude to my supervisor Dr. Leila Mostaço-Guidolin. Her guidance, effort, and determination throughout the course of my studies, and in the writing of this thesis, have been invaluable. Especially during this period of history we find ourselves: the COVID-19 Pandemic, where studies felt like an uphill battle. She helped me ride out the storm, and all the while I gained valuable experience and knowledge that I'm sure will be helpful in the bright future. More so than that, she has been a source of inspiration, in her work ethic, dedication to her profession, and seemingly tireless ability to find time for her students in the midst of her own busy schedule.While I may not have met them, or know them, as much as I would have liked to due to the restrictions of the pandemic, I would like to thank my lab group, the BioImaging and Tissue Eng Lab. From the rare, and very much enjoyable, in-person meeting, to the mid-week Zoom meetings, it was always a pleasure to connect. I would especially like to thank Natasha Kunchur for her advice while I was writing this thesis. I would like to extend my thanks to Carleton University as a whole, an institution I have grown to know rather well in the seven years I have spent studying within iii its walls (two from the comfort of home), for its financial support during this degree.To my friends, for their constant support during my studies, which once appeared like they would never finish. At the times where overwhelming stress and anxiety became too much to bear, they would always be there to provide an ear to hear my complaints, a shoulder to cry on, or provide a much needed distraction.Finally, to my family, without whose support this thesis would never have been completed. Words cannot express how much gratitude I have for your support, guidance and understanding over the last few years. Especially my parents, Christopher Poole and Judith Poole, to whom I dedicate this thesis.