Comparative suitability of CFDA‐SE and rhodamine 6G for <i>in vivo</i> assessment of leukocyte‐endothelium interactions

Jbeily, Nayla; Claus, Ralf A.; Dahlke, Katja; Neugebauer, Ute; Bauer, Michael; Gonnert, Falk A.

doi:10.1002/jbio.201300179

Cited by 9 publications

(6 citation statements)

References 17 publications

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“…The potential of rhodamine 6G and carboxyfluorescein diacetate succinimidyl ester (CFDA‐SE) in labeling different leukocytes subpopulations as well as their fluorescence intensities were assessed by flow cytometry. CFDA‐SE revealed to be superior in labeling leukocytes for intravital fluorescence microscopy with respect to image quality . Yet, to date, flow cytometry systems have lacked the ability to capture complex fluorescence dynamics due to the transient nature of flowing cells.…”

Section: Cell and Bacteria Characterizationmentioning

confidence: 99%

Modern trends in biophotonics for clinical diagnosis and therapy to solve unmet clinical needs

Krafft

2016

Journal of Biophotonics

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This contribution covers recent original research papers in the biophotonics field. The content is organized into main techniques such as multiphoton microscopy, Raman spectroscopy, infrared spectroscopy, optical coherence tomography and photoacoustic tomography, and their applications in the context of fluid, cell, tissue and skin diagnostics. Special attention is paid to vascular and blood flow diagnostics, photothermal and photodynamic therapy, tissue therapy, cell characterization, and biosensors for biomarker detection.

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Section: Cell and Bacteria Characterizationmentioning

confidence: 99%

Modern trends in biophotonics for clinical diagnosis and therapy to solve unmet clinical needs

Krafft

2016

Journal of Biophotonics

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“…We used 0.05 % Rhodamine 6G for assessment of leukocyte adhesion, rolling and migration across pial brain vessels [ 18 ]. The dye injection technique does affect the outcome of imaging acquisition, leaving the intravenous injection of any labeling agent as the preferable method [ 21 ]. We found that retro-orbital injection was the preferred method for injection of the fluorophore chosen, as has been demonstrated for other compounds that need to be delivered intravenously [ 22 ].…”

Section: Methodsmentioning

confidence: 99%

Craniula: A cranial window technique for prolonged imaging of brain surface vasculature with simultaneous adjacent intracerebral injection

Zuluaga-Ramirez

Rom

Persidsky

2015

Fluids Barriers CNS

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BackgroundImaging of the brain surface vasculature following inflammatory insults is critical to study structural and functional changes in the living brain under normal and pathological conditions. Although there have been published reports relating to the changes that occur in the blood brain barrier (BBB) during the inflammatory process, the ability to visualize and track such changes in vivo and over time has proven to be problematic. Different techniques have been used to achieve visualization of pial vessels, but the approach has limits, which can jeopardize the well-being of the animals. Development of the cranial window technique provided a major advance in the acquisition of live images of the brain vasculature and its response to different insults and treatments.MethodsWe describe in detail a protocol for delivery of a localized inflammatory insult to the mouse brain via a craniula (cranial window and adjacent cannula) and subsequent imaging of the mouse brain vasculature by intravital microscopy and two-photon laser scanning microscopy. The surgical implantation of the craniula can be completed in 30-45 min and images can be acquired immediately and for several months thereafter. The technique is minimally invasive and permits serial injections directly to the brain, thereby allowing longitudinal imaging studies. The craniula technique permits the study of structural and functional changes of the BBB following inflammatory insult and as such has wide application to neuroscience research.

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“…120,126 However, this approach presents limitations as a result of parenchymal tissue accumulation and phototoxic-induced hemodynamic alterations. 127,128 A promising new dye, which apparently avoids these disadvantages, recently was tested for intravital imaging of leukocytes in the liver. 127 Carboxyfluorescein diacetate succinimidyl ester does not compromise leukocyte circulation and recruitment, 129 and in vivo staining of leukocytes showed a high imaging quality of leukocytes.…”

Section: Endothelial Dysfunctionmentioning

confidence: 99%

“…127,128 A promising new dye, which apparently avoids these disadvantages, recently was tested for intravital imaging of leukocytes in the liver. 127 Carboxyfluorescein diacetate succinimidyl ester does not compromise leukocyte circulation and recruitment, 129 and in vivo staining of leukocytes showed a high imaging quality of leukocytes. 127 However, carboxyfluorescein diacetate succinimidyl ester has not been used for in vivo imaging of leukocytes in the kidney yet.…”

Section: Endothelial Dysfunctionmentioning

confidence: 99%

Just Look! Intravital Microscopy as the Best Means to Study Kidney Cell Death Dynamics

Schießl

Hammer

Riquier‐Brison

et al. 2016

Seminars in Nephrology

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Summary Kidney cell death plays a key role in the progression of life-threatening renal diseases, such as acute kidney injury and chronic kidney disease. Injured and dying epithelial and endothelial cells take part in complex communication with the innate immune system, which drives the progression of cell death and the decrease in renal function. To improve our understanding of kidney cell death dynamics and its impact on renal disease, a study approach is needed that facilitates the visualization of renal function and morphology in real time. Intravital multiphoton microscopy of the kidney has been used for more than a decade and made substantial contributions to our understanding of kidney physiology and pathophysiology. It is a unique tool that relates renal structure and function in a time- and spatial-dependent manner. Basic renal function, such as microvascular blood flow regulation and glomerular filtration, can be determined in real time and homeostatic alterations, which are linked inevitably to cell death and can be depicted down to the subcellular level. This review provides an overview of the available techniques to study kidney dysfunction and inflammation in terms of cell death in vivo, and addresses how this novel approach can be used to improve our understanding of cell death dynamics in renal disease.

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Comparative suitability of CFDA‐SE and rhodamine 6G for in vivo assessment of leukocyte‐endothelium interactions

Cited by 9 publications

References 17 publications

Modern trends in biophotonics for clinical diagnosis and therapy to solve unmet clinical needs

Modern trends in biophotonics for clinical diagnosis and therapy to solve unmet clinical needs

Craniula: A cranial window technique for prolonged imaging of brain surface vasculature with simultaneous adjacent intracerebral injection

Just Look! Intravital Microscopy as the Best Means to Study Kidney Cell Death Dynamics

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