Just Look! Intravital Microscopy as the Best Means to Study Kidney Cell Death Dynamics

Schießl, Ina Maria; Hammer, Anna; Riquier‐Brison, Anne; Peti‐Peterdi, Janos

doi:10.1016/j.semnephrol.2016.03.009

Cited by 16 publications

(13 citation statements)

References 145 publications

(260 reference statements)

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“…Imaging technologies need to be adapted for time‐dependant phenomena. Intravital microscopy has been used to look at the dynamics of renal cell death and could be used for other types of dynamic observation with adapted labelling . Live‐cell correlative light‐electron microscopy (live‐cell CLEM) offers the possibility of simultaneously recording the dynamics of subcellular components whilst imaging their structural properties .…”

Section: Discussionmentioning

confidence: 99%

Cellular plasticity: A mechanism for homeostasis in the kidney

et al. 2020

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Cellular plasticity is a topical subject with interest spanning a wide range of fields from developmental biology to regenerative medicine. Even the nomenclature is a subject of debate, and the underlying mechanisms are still under investigation. On top of injury repair, cell plasticity is a constant physiological process in adult organisms and tissues, in response to homeostatic challenges. In this review we discuss two examples of plasticity for the maintenance of homeostasis in the renal systemnamely the renin-producing juxtaglomerular cells (JG cells) and cortical collecting duct (CCD) cells. JG cells show plasticity through recruitment mechanisms, answering the demand for an increase in renin production. In the CCD, cells appear to have the ability to transdifferentiate between principal and intercalated cells to help maintain the highly regulated solute transport levels of that segment. These two cases highlight the complexity of plasticity processes and the role they can play in the kidney. K E Y W O R D Scollecting duct, JG cells, kidney, plasticity, renin 2 of 12 | ASSMUS et Al.

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Section: Discussionmentioning

confidence: 99%

Cellular plasticity: A mechanism for homeostasis in the kidney

et al. 2020

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“…Kidney IVM can be used to investigate a renal injury, IR injury, dysfunction, inflammation, cell death, microvascular blood flow, glomerular filtration and podocyte migration ( Russo et al, 2007 ; Devi et al, 2013 ; Hackl et al, 2013 ; Hall et al, 2013 ; Schießl et al, 2016b ).…”

Section: Models/operation Techniquesmentioning

confidence: 99%

Actually Seeing What Is Going on – Intravital Microscopy in Tissue Engineering

Vaghela

Arkudas

Horch

et al. 2021

Front. Bioeng. Biotechnol.

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Intravital microscopy (IVM) study approach offers several advantages over in vitro, ex vivo, and 3D models. IVM provides real-time imaging of cellular events, which provides us a comprehensive picture of dynamic processes. Rapid improvement in microscopy techniques has permitted deep tissue imaging at a higher resolution. Advances in fluorescence tagging methods enable tracking of specific cell types. Moreover, IVM can serve as an important tool to study different stages of tissue regeneration processes. Furthermore, the compatibility of different tissue engineered constructs can be analyzed. IVM is also a promising approach to investigate host reactions on implanted biomaterials. IVM can provide instant feedback for improvising tissue engineering strategies. In this review, we aim to provide an overview of the requirements and applications of different IVM approaches. First, we will discuss the history of IVM development, and then we will provide an overview of available optical modalities including the pros and cons. Later, we will summarize different fluorescence labeling methods. In the final section, we will discuss well-established chronic and acute IVM models for different organs.

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“…Drops in ATP levels would have occurred early after onset of ischemia and driven actin cytoskeletal derangements that favor the non-filamentous form of actin, as the cytoskeleton requires ATP to remain in a filamentous form [16]. In summary, due to the complex nature of the kidney, in vivo studies have relied on exogenous probes to investigate the underlying nature of renal tubular morphological and functional processes [26,27]. To extend this approach, this study demonstrates the utility of the combinative use of HGD and intravital two-photon microscopy to track the dynamic remodeling of the actin cytoskeleton.…”

Section: Real-time In Vivo Imaging Of Actin Dysregulation At the Onsementioning

confidence: 99%

Intravital imaging of real-time endogenous actin dysregulation in proximal and distal tubules at the onset of severe ischemia-reperfusion injury

Corridon

Karam

Khraibi

et al. 2021

Preprint

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Severe renal ischemia-reperfusion injury (IRI) can lead to acute and chronic kidney dysfunction. Cytoskeletal modifications are among the main effects of this condition. The majority of studies that have contributed to the current understanding of IRI have relied on histological analyses using exogenous probes after the fact. Here we report the successful real-time visualization of actin cytoskeletal alterations in live proximal and distal tubules that arise at the onset of severe IRI. To achieve this, we induced fluorescent actin expression in these segments in rats with hydrodynamic gene delivery (HGD). Using intravital two-photon microscopy we then tracked and quantified endogenous actin dysregulation that occurred by subjecting these animals to 60 minutes of bilateral renal ischemia. Rapid (by 1-hour post-reperfusion) and significant (up to 50%) declines in actin content were observed. The decline in fluorescence within proximal tubules was significantly greater than that observed in distal tubules. Actin-based fluorescence was not recovered during the measurement period extending 24 hours post-reperfusion. Such injury decimated the renal architecture, in particular, actin brush borders, and hampered the reabsorptive and filtrative capacities of these tubular compartments. Thus, for the first time, we show that the combination of HGD and intravital microscopy can serve as an experimental tool to better understand how IRI modifies the cytoskeleton in vivo and provide an extension to current histopathological techniques.

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Just Look! Intravital Microscopy as the Best Means to Study Kidney Cell Death Dynamics

Cited by 16 publications

References 145 publications

Cellular plasticity: A mechanism for homeostasis in the kidney

Cellular plasticity: A mechanism for homeostasis in the kidney

Actually Seeing What Is Going on – Intravital Microscopy in Tissue Engineering

Intravital imaging of real-time endogenous actin dysregulation in proximal and distal tubules at the onset of severe ischemia-reperfusion injury

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