Comparative toxicity, anticholinesterase action and metabolism of methyl parathion and parathion in sunfish and mice

Benke, G.M.; Cheever, Kenneth L.; Mirer, Franklin E.; Murphy, Sheldon D.

doi:10.1016/0041-008x(74)90135-5

Cited by 158 publications

(30 citation statements)

References 19 publications

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“…These pathways for fenitrothion in fish are found in other biological systems in vivo (£) ; the former 3 pathways are similar to those found with methylparathion in s un fish in_ vitro (10) , and glucuronic acid conjugation was reported to occur in metabolism in_ vivo of 3-trifluoromethyl-4-nitrophenol in rainbow trout ( 11).…”

Section: Metabolism and Bioaccumulation In Vivomentioning

confidence: 76%

Metabolism of Organophosphorus Insecticides in Aquatic Organisms, with Special Emphasis on Fenitrothion

Miyamoto

Takimoto

Mihara

1979

ACS Symposium Series

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The organophosphorus compounds constitute one major group of insecticides, and a certain portion thereof may be transported to the aquatic environment resulting either from the actual use on paddy fields or from unavoidable transmittance to waterways. However, possibly because of its relatively shorter persistence, the translocation and transformation of organophosphorus compounds in the aquatic environment has not been extensively investigated as compared with more persistent organοchlorine compounds.Fenitrothion, O,O-dimethyl O-(3-methyl-4-nitrophenyl) phosphorothioate, is widely used for the control of paddy field insects and forest protection in several countries, and since it is rather highly toxic to some aquatic organisms (LC 50 after 48hr exposure, 1.28 ppm for rainbow trout, 2.72 ppm for bluegill, 4.4 ppm for carp, LC 50 after 3 hr exposure, 0.0092 ppm for daphnia and no-effect dosage after 4 week exposure, 0.02 ppm for carp) (1, 2), the knowledge on degradation and metabolism of the compound in the aquatic environment is important for assessing short-term and long-term impacts on the non-target aquatic organisms.In this article metabolism and bioaccumulation of fenitro thion in several aquatic species are dealt with under laboratory conditions. Metabolism in vitroTo acquire information on the intrinsic metabolic activity of aquatic organisms, liver of carp (Cyprinus carpio Linnaeus), rainbow trout (Salmo gairdneri) and freshwater snail (Cipangopaludina japonica Martens) was dissected out, homogenized in 0.1M phosphate buffer, pH 7.5, and centrifuged at 105,000 g for 60 min to obtain the microsome-equivalent (described as the microsomal fraction hereafter) fraction. The protein content of microsomal and sub microsomal (supernatant fractions by Lowry's method, micro somal P-450 content (3), activity of aniline hydroxylase (4) and aminopyrine N-demethylase (_5) were determined. Table I shows the results which reveal that the drug-0-8412-0489-6/79/47-099-003$05.00/0

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Section: Metabolism and Bioaccumulation In Vivomentioning

confidence: 76%

Metabolism of Organophosphorus Insecticides in Aquatic Organisms, with Special Emphasis on Fenitrothion

Miyamoto

Takimoto

Mihara

1979

ACS Symposium Series

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“…1.11.1.9) by the method of Lawrence and Burk (1976), and glutathione reductase (GR) (EC.1.6.4.2) by the method of David and Richard (1983) were determined. Glutathione content (GSH) was assayed according to Benke and Cheevar (1974). Protein was estimated with bovine serum albumin (BSA) as standard (Bradford 1976).…”

Section: Biochemical Analysesmentioning

confidence: 99%

In vivo protective effect of dietary curcumin in fish Anabas testudineus (Bloch)

Manju

Akbarsha²,

Oommen

2011

Fish Physiol Biochem

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The present study describes, for the first time, the protective effect of natural curcumin in vivo in a lower vertebrate, a teleost, Anabas testudineus (Bloch). Two doses of curcumin 0.5 and 1% were supplemented in the 40% protein feed and fed to fish for the periods, 2 and 8 weeks. The antioxidant status, protein content, and the tissue structure in experimental fish were examined after the short-term and long-term feeding. In all the curcumin fed groups, the lipid peroxidation product, thiobarbituric acid reactive substances content either decreased or unaffected. The glutathione content increased while the antioxidant enzyme activity pattern varied with time and dose. The histological analysis also confirmed the safety of curcumin retaining the normal arrangement of hepatocytes, hepatopancreas, macrophage-melanocyte centers in Anabas. The improved antioxidant status and protein content suggest a favorable effect for curcumin in cultured fish.

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“…Lipid peroxidation in the liver was determined by measuring the peroxidation products. Thiobarbituric acid reactive substances (TBARS) content was assayed using the method of Nichans and Samuelsson (1968) and glutathione content (GSH) according to Benke and Cheevar (1974). Protein was estimated using bovine serum albumin (BSA) as the standard (Bradford 1976).…”

Section: Biochemical Analysismentioning

confidence: 99%

Curcumin analogue inhibits lipid peroxidation in a freshwater teleost, Anabas testudineus (Bloch)—an in vitro and in vivo study

Manju

Sherin

Rajasekharan

et al. 2008

Fish Physiol Biochem

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The effect of a synthetic curcumin analogue (salicylcurcumin) on fish lipid peroxidation was investigated in both in vitro and in vivo conditions using a teleost model Anabas testudineus (Bloch). Curcumin analogue inhibited the formation of lipid peroxidation products and thiobarbituric acid reactive substances (TBARS) content at the three concentrations (10(-2) M, 10(-3) M and 10(-4) M) in vitro. TBARS content was reduced by 80% in the liver and 68% in brain by the higher concentration of salicylcurcumin. For in vivo study, salicylcurcumin (0.5%) was supplemented along with the basal feed for a period of 60 days. It produced a 60% reduction in liver TBARS content. The antioxidant enzyme superoxide dismutase (SOD) was stimulated, whereas catalase (CAT) and glutathione peroxidase (GPx) were inhibited. Glutathione (GSH) was reduced and glutathione reductase (GR) unchanged. Even though there was an increase in SOD activity, the CAT and GPx did not increase accordingly, maybe due to the direct scavenging of H(2)O(2) by salicylcurcumin. The protein content also increased in the curcumin-fed animals, indicating a positive growth-promoting effect. Therefore, it would be beneficial to supplement salicylcurcumin along with the aquaculture feed in order to help the fish to cope with adverse conditions in the environment. This would increase the survival rate, disease resistance and ultimately the growth rate.

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Comparative toxicity, anticholinesterase action and metabolism of methyl parathion and parathion in sunfish and mice

Cited by 158 publications

References 19 publications

Metabolism of Organophosphorus Insecticides in Aquatic Organisms, with Special Emphasis on Fenitrothion

Metabolism of Organophosphorus Insecticides in Aquatic Organisms, with Special Emphasis on Fenitrothion

In vivo protective effect of dietary curcumin in fish Anabas testudineus (Bloch)

Curcumin analogue inhibits lipid peroxidation in a freshwater teleost, Anabas testudineus (Bloch)—an in vitro and in vivo study

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