Comparative transcriptional analysis of Capsicum flower buds between a sterile flower pool and a restorer flower pool provides insight into the regulation of fertility restoration

Wei, Bingqiang; Wang, Lanlan; Bosland, Paul W.; Zhang, Gaoyuan; Zhang, Ru

doi:10.1186/s12864-019-6210-3

Cited by 17 publications

(7 citation statements)

References 84 publications

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“…Furthermore, some specific DEGs that have a significant impact on plant sex regulation and flower development have also been discovered. Many articles on the sequencing of plant male and female flower buds show that both sugar and lipid metabolism are pathways in which DEGs are more enriched [53][54][55]. A large number of genes related to glycolipid synthesis have also been found to be involved in the regulation of plant sex and the development of floral organs.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis of Morus alba L. Flower Reveals Important Genes of Floral Sex Differentiation

Hou

Deng

et al. 2022

Horticulturae

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Mulberry (Morus alba L.) is a perennial woody plant with significant economic benefits and ecological value. The floral character of mulberry has an important impact on the yield and quality to its fruits and leaves. However, little is known about the molecular mechanism of mulberry floral differentiation still now. The transcriptome data were obtained via Illumina HiSeq high-throughput sequencing from male and female inflorescences of the monoecious mulberry. A total of 26.21 Gb clean data were obtained, and as many as 100,177 unigenes with an average length of 821.66 bp were successfully assembled. In comparative-omics analysis, 1717 differentially expressed genes (DEGs) were identified between male and female flowers and only a quarter of the DEGs were highly expressed in female flowers. The KEGG pathway enrichment analysis revealed that DEGs were involved in glucose and lipid metabolism, hormone signal transduction, and the regulation of related transcription factors. In addition, many DEGs related to flower development and plant sex differentiation have also been detected, such as PMADS1/2, AGAMOUS, FLOWERING LOCUS T (FT), APETALA 2 (AP2), TASSELSEED2 (TS2), and ARABIDOPSIS RESPONSE REGULATOR 17 (ARR17). Finally, the expression patterns of selected 20 DEGs were validated by q-PCR and the results showed that the transcriptome data were highly reliable. This study shows that the differentiation of male and female flowers of mulberry is affected and regulated by multiple factors, with transcription factors and hormone signals playing a key role. Briefly, the current data provide comprehensive insights into the mulberry tree’s floral differentiation as well as a bioinformatics framework for the development of molecular breeding of mulberry.

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Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis of Morus alba L. Flower Reveals Important Genes of Floral Sex Differentiation

Hou

Deng

et al. 2022

Horticulturae

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“…It is well known that celluloses and hemicelluloses are the important components of pollen intine [66]. The toughness and strength of cell wall were affected by the cellulose which consisted of a β-1,4-glucan long chain.…”

Section: Discussionmentioning

confidence: 99%

Conjunctive Analyses of BSA-Seq and BSR-Seq Unveil the Msβ-GAL and MsJMT as Key Candidate Genes for Cytoplasmic Male Sterility in Alfalfa (Medicago sativa L.)

Zhou

Wang

et al. 2022

IJMS

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Knowing the molecular mechanism of male sterility in alfalfa is important to utilize the heterosis more effectively. However, the molecular mechanisms of male sterility in alfalfa are still unclear. In this study, the bulked segregant analysis (BSA) and bulked segregant RNA-seq (BSR) were performed with F2 separation progeny to study the molecular mechanism of male sterility in alfalfa. The BSA-seq analysis was located in a candidate region on chromosome 5 containing 626 candidate genes which were associated with male sterility in alfalfa, while the BSR-seq analysis filtered seven candidate DEGs related to male sterility, and these candidate genes including EF-Tu, β-GAL, CESA, PHGDH, and JMT. The conjunctive analyses of BSR and BSA methods revealed that the genes of Msβ-GAL and MsJMT are the common detected candidate genes involved in male sterility in alfalfa. Our research provides a theory basis for further study of the molecular mechanism of male sterility in alfalfa and significant information for the genetic breeding of Medicago sativa.

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“…The CMS line 8A ( C. annuum L.), the restorer line R1 ( C. annuum L.), and two extreme pools, a sterile pool (SP) and a restorer pool (RP), were described in our previously published articles 81 . The methods of transcriptome sequencing, quantification of gene expression levels and DEG analysis were the same as in the previous articles 81 .…”

Section: Methodsmentioning

confidence: 99%

A predicted NEDD8 conjugating enzyme gene identified as a Capsicum candidate Rf gene using bulk segregant RNA sequencing

et al. 2020

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Cytoplasmic male sterility (CMS) is an important tool for producing F1 hybrids, which can exhibit heterosis. The companion system, restorer-of-fertility (Rf), is poorly understood at the molecular level and would be valuable in producing restorer lines for hybrid seed production. The identity of the Rf gene in Capsicum (pepper) is currently unclear. In this study, using bulked segregant RNA sequencing (BSR-seq), a strong candidate Rf gene, Capana06g002866, which is annotated as a NEDD8 conjugating enzyme E2, was identified. Capana06g002866 has an ORF of 555 bp in length encoding 184 amino acids; it can be cloned from F1 plants from the hybridization of the CMS line 8A and restorer line R1 but is not found in CMS line 8A. With qRT-PCR validation, Capana06g002866 was found to be upregulated in restorer accessions compared to sterile accessions. The relative expression in flower buds increased with the developmental stage in F1 plants, while the expression was very low in all flower bud stages of the CMS lines. These results provide new insights into the Rf gene in pepper and will be useful for other crops utilizing the CMS system.

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Comparative transcriptional analysis of Capsicum flower buds between a sterile flower pool and a restorer flower pool provides insight into the regulation of fertility restoration

Cited by 17 publications

References 84 publications

Transcriptome Analysis of Morus alba L. Flower Reveals Important Genes of Floral Sex Differentiation

Transcriptome Analysis of Morus alba L. Flower Reveals Important Genes of Floral Sex Differentiation

Conjunctive Analyses of BSA-Seq and BSR-Seq Unveil the Msβ-GAL and MsJMT as Key Candidate Genes for Cytoplasmic Male Sterility in Alfalfa (Medicago sativa L.)

A predicted NEDD8 conjugating enzyme gene identified as a Capsicum candidate Rf gene using bulk segregant RNA sequencing

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